##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727099.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3199668
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.463004911759594	31.0	30.0	31.0	28.0	34.0
2	30.849358745969894	31.0	31.0	34.0	28.0	34.0
3	31.17887918371531	31.0	31.0	34.0	28.0	34.0
4	35.073130712311404	35.0	35.0	37.0	32.0	37.0
5	34.785947479551005	35.0	35.0	37.0	32.0	37.0
6	34.68310805996122	35.0	35.0	37.0	32.0	37.0
7	34.52511666835434	35.0	35.0	37.0	30.0	37.0
8	34.51503374725128	35.0	35.0	37.0	30.0	37.0
9	35.882291850279465	37.0	35.0	39.0	30.0	39.0
10	35.71731817176032	37.0	35.0	39.0	30.0	39.0
11	35.718447663945135	37.0	35.0	39.0	30.0	39.0
12	35.55615488856969	37.0	35.0	39.0	30.0	39.0
13	35.66843997564747	37.0	35.0	39.0	30.0	39.0
14	36.41198618106628	38.0	35.0	40.0	30.0	41.0
15	36.409636249760915	38.0	35.0	40.0	30.0	41.0
16	36.22482738834154	38.0	34.0	40.0	30.0	41.0
17	36.21910898255694	38.0	34.0	40.0	30.0	41.0
18	36.092820880166315	38.0	34.0	40.0	29.0	41.0
19	36.024774445348704	38.0	34.0	40.0	28.0	41.0
20	36.49158756470984	38.0	35.0	40.0	30.0	41.0
21	36.59249053339284	38.0	35.0	40.0	30.0	41.0
22	36.59445104929637	38.0	35.0	40.0	30.0	41.0
23	36.48701709052314	38.0	35.0	40.0	30.0	41.0
24	36.35401954202749	38.0	35.0	40.0	30.0	41.0
25	36.0536574419596	38.0	34.0	40.0	28.0	41.0
26	35.836705245669236	38.0	34.0	40.0	27.0	41.0
27	35.68070781093538	38.0	34.0	40.0	27.0	41.0
28	35.40468854893695	38.0	34.0	40.0	27.0	41.0
29	35.24560891942539	38.0	34.0	40.0	26.0	41.0
30	34.89986148562913	38.0	33.0	40.0	25.0	41.0
31	34.73630482912602	38.0	33.0	40.0	25.0	41.0
32	34.507539844758895	37.0	33.0	40.0	24.0	41.0
33	34.47006845710242	37.0	33.0	40.0	24.0	41.0
34	34.27272266997701	37.0	33.0	40.0	24.0	41.0
35	33.88254468901148	37.0	32.0	40.0	22.0	41.0
36	33.87713662792515	37.0	32.0	40.0	23.0	41.0
37	33.69049132597507	37.0	32.0	40.0	21.0	41.0
38	33.55137439259323	37.0	31.0	40.0	21.0	41.0
39	33.404494153768454	37.0	31.0	40.0	20.0	41.0
40	33.17287574835889	37.0	31.0	40.0	18.0	41.0
41	32.94312816204681	36.0	31.0	40.0	17.0	41.0
42	32.741916036288764	36.0	30.0	40.0	15.0	41.0
43	32.56261430873453	36.0	30.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	9.0
11	17.0
12	31.0
13	33.0
14	87.0
15	138.0
16	277.0
17	513.0
18	1165.0
19	2176.0
20	4199.0
21	7213.0
22	11923.0
23	18973.0
24	26867.0
25	38194.0
26	50688.0
27	66414.0
28	85801.0
29	105507.0
30	129286.0
31	156303.0
32	185794.0
33	219132.0
34	258866.0
35	299040.0
36	351885.0
37	408919.0
38	464260.0
39	305958.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.15113568032684	22.762611620955674	14.603515114693149	25.482737584024346
2	17.85078951941264	26.197436734061156	31.054784433885015	24.89698931264119
3	19.97222836869325	27.10846875363319	30.274828513458274	22.644474364215288
4	15.998534847990479	18.23598573351985	31.51255067713275	34.25292874135691
5	13.333320832036325	40.55539512224393	32.134146417690836	13.977137628028908
6	33.46991000316283	36.29939106182267	15.054561910798245	15.176137024216263
7	27.00477049493885	33.04855378745545	21.04621479478496	18.90046092282074
8	28.316187804484716	35.28416073167591	19.910909506861337	16.488741956978036
9	28.4566711296297	14.665677814073211	19.06832208841667	37.80932896788042
10	17.945299324804946	29.39111182785214	31.21739505473693	21.446193792605982
11	34.73382238407235	23.691364229038765	19.832151335701077	21.74266205118781
12	21.184229113770552	29.478464640706477	28.46698469966259	20.87032154586038
13	30.329177902207356	20.586510850500737	25.31843928807614	23.76587195921577
14	22.945693115660752	22.479394737203982	25.333128312062374	29.241783835072887
15	26.591571375530208	27.9575255932803	21.88480179818656	23.56610123300292
16	23.123524065621808	26.926918667811783	26.32007445772499	23.629482808841416
17	23.83444157331323	28.906592809003932	25.254901446025023	22.00406417165781
18	24.344838276971238	24.80226073455121	27.348368643246733	23.504532345230817
19	24.96308992057926	27.83707559659315	25.934784483890205	21.265049998937393
20	23.7621215701129	25.68253956347971	27.527856015061563	23.027482851345827
21	23.872039224069496	27.809228957504338	26.39764500566934	21.921086812756823
22	24.124659183390275	27.234888119642413	24.383186005548076	24.257266691419236
23	22.187770731213362	27.0392115682002	25.85052574204574	24.9224919585407
24	22.435140145790125	29.346732223468187	25.423325169986388	22.794802460755303
25	23.690020339610236	27.386310079670768	24.320835786712873	24.60283379400613
26	23.52987872491771	27.309458356304468	25.844993918119002	23.31566900065882
27	24.42669051914136	27.423688957729365	24.557360326133836	23.592260196995436
28	23.027076559193016	27.969808117592198	26.134492703618	22.868622619596785
29	23.229910103173204	27.287643593022775	26.590477512041875	22.891968791762146
30	22.87306058003518	28.726667891793774	26.710177430908455	21.69009409726259
31	24.224513293254173	27.454942200253274	24.705469442454657	23.615075064037892
32	21.23110897755642	28.623750964162532	25.768329714207848	24.376810344073196
33	22.296532015196576	27.69887375815241	26.54244127828262	23.46215294836839
34	22.05406935969607	27.914927423720208	26.447712700192643	23.583290516391074
35	22.651068798387833	28.63268939152437	26.666704170557697	22.0495376395301
36	23.33260825810678	27.971433286203446	25.537649531138857	23.158308924550923
37	22.488989482658823	28.228991257843	26.262599744723513	23.019419514774658
38	23.037515142196003	27.474162944405485	26.682705830729937	22.805616082668575
39	23.18287397317472	26.169683854699926	26.519813930695307	24.12762824143005
40	22.938067324484916	27.394092137059218	26.80253076256662	22.865309775889248
41	21.52510822997886	27.72062601494905	27.03455483506414	23.719710920007948
42	23.037202609770763	28.071943714160348	26.978017719338382	21.91283595673051
43	22.558621707002104	26.28269557966639	27.14744154706051	24.011241166271
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	764.0
1	1165.5
2	1567.0
3	1670.0
4	1773.0
5	1773.0
6	2293.0
7	2813.0
8	4225.5
9	5638.0
10	9067.0
11	12496.0
12	12496.0
13	25110.0
14	37724.0
15	46234.5
16	54745.0
17	44021.5
18	33298.0
19	33298.0
20	37774.0
21	42250.0
22	39117.5
23	35985.0
24	38872.5
25	41760.0
26	41760.0
27	46444.5
28	51129.0
29	54569.5
30	58010.0
31	62628.5
32	67247.0
33	67247.0
34	78102.5
35	88958.0
36	102865.0
37	116772.0
38	131848.0
39	146924.0
40	146924.0
41	156600.0
42	166276.0
43	179525.5
44	192775.0
45	221521.5
46	250268.0
47	250268.0
48	321302.0
49	392336.0
50	378020.0
51	363704.0
52	295734.5
53	227765.0
54	227765.0
55	206005.5
56	184246.0
57	174880.5
58	165515.0
59	152996.5
60	140478.0
61	140478.0
62	119317.0
63	98156.0
64	84677.0
65	71198.0
66	60520.0
67	49842.0
68	49842.0
69	40991.5
70	32141.0
71	28811.0
72	25481.0
73	20448.0
74	15415.0
75	15415.0
76	11849.5
77	8284.0
78	7077.5
79	5871.0
80	4642.5
81	3414.0
82	3414.0
83	2887.0
84	2360.0
85	1924.5
86	1489.0
87	1498.0
88	1507.0
89	1507.0
90	1054.0
91	601.0
92	470.0
93	339.0
94	298.0
95	257.0
96	257.0
97	152.0
98	47.0
99	48.5
100	50.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3199668.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.77740724251537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.64410450642687	42.133640606944404
2	7.9221632772740564	7.886894953891418
3	2.643223763620085	3.947184771444334
4	1.2918975919840219	2.5722925020725445
5	0.7924853167797307	1.9723932173529226
6	0.5136552089134004	1.5341054709792987
7	0.3565716590949616	1.2424448880136483
8	0.26737225062525316	1.0647277923776888
9	0.2002753012641151	0.8972266708477181
>10	1.1802204023658474	10.671891085557576
>50	0.09204149265769745	3.1920079339755674
>100	0.07712387506147328	7.967036352266472
>500	0.010978500281799291	3.7268138839430356
>1k	0.006940431212538811	6.343831072981944
>5k	6.309482920489829E-4	2.2789839700813355
>10k+	3.1547414602449143E-4	2.5685248272699828
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	22920	0.7163243186480597	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16042	0.5013645165685939	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	15935	0.4980204196185355	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	15472	0.4835501683299642	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	11413	0.35669325692540604	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9502	0.29696831046221045	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	9043	0.2826230721437349	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	8281	0.2588081013405141	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	7793	0.24355651898884512	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	7119	0.2224918335277285	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	7070	0.2209604246440568	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	6435	0.2011146156413728	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	6373	0.19917691460489026	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5580	0.17439309328342814	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	5367	0.1677361526258349	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	4574	0.14295233130437285	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	4542	0.14195222754360765	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	4479	0.1399832732646012	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3403	0.10635478430887206	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3321	0.10379201842191127	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	3229	0.10091672010971138	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	3224	0.10076045389709183	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	3208	0.10026040201670924	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.1253242523911856E-5	0.0
3	0.0	0.0	0.0	3.1253242523911856E-5	0.0
4	0.0	0.0	0.0	3.1253242523911856E-5	0.0
5	0.0	0.0	0.0	3.1253242523911856E-5	0.0
6	0.0	0.0	0.0	3.1253242523911856E-5	3.1253242523911856E-5
7	0.0	0.0	0.0	3.1253242523911856E-5	3.1253242523911856E-5
8	3.1253242523911856E-5	0.0	0.0	3.1253242523911856E-5	3.1253242523911856E-5
9	3.1253242523911856E-5	0.0	0.0	3.1253242523911856E-5	3.1253242523911856E-5
10	3.1253242523911856E-5	0.0	0.0	3.1253242523911856E-5	3.1253242523911856E-5
11	3.1253242523911856E-5	0.0	0.0	1.2501297009564742E-4	3.1253242523911856E-5
12	3.1253242523911856E-5	0.0	0.0	2.5002594019129485E-4	3.1253242523911856E-5
13	3.1253242523911856E-5	0.0	0.0	3.1253242523911857E-4	3.1253242523911856E-5
14	3.1253242523911856E-5	0.0	0.0	4.3754539533476597E-4	3.1253242523911856E-5
15	3.1253242523911856E-5	0.0	0.0	8.438375481456201E-4	3.1253242523911856E-5
16	3.1253242523911856E-5	0.0	0.0	0.0019064477939586232	3.1253242523911856E-5
17	3.1253242523911856E-5	0.0	0.0	0.0036566293752976872	3.1253242523911856E-5
18	3.1253242523911856E-5	0.0	0.0	0.004625479893538955	3.1253242523911856E-5
19	3.1253242523911856E-5	0.0	0.0	0.006813206870212784	3.1253242523911856E-5
20	3.1253242523911856E-5	0.0	0.0	0.00878216114921923	3.1253242523911856E-5
21	3.1253242523911856E-5	0.0	0.0	0.01381393319556904	3.1253242523911856E-5
22	3.1253242523911856E-5	0.0	0.0	0.022971133255075216	3.1253242523911856E-5
23	3.1253242523911856E-5	0.0	0.0	0.04194185146708971	9.375972757173557E-5
24	3.1253242523911856E-5	0.0	0.0	0.07682047012377534	9.375972757173557E-5
25	3.1253242523911856E-5	0.0	0.0	0.10179181090038092	9.375972757173557E-5
26	6.250648504782371E-5	0.0	0.0	0.13091983293266676	9.375972757173557E-5
27	6.250648504782371E-5	0.0	0.0	0.1734242427651869	9.375972757173557E-5
28	6.250648504782371E-5	0.0	0.0	0.24296270738089076	9.375972757173557E-5
29	6.250648504782371E-5	0.0	0.0	0.30693809482733836	9.375972757173557E-5
30	6.250648504782371E-5	0.0	0.0	0.3999477445785	9.375972757173557E-5
31	6.250648504782371E-5	0.0	0.0	0.5780912269647976	9.375972757173557E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGT	30	3.600902E-4	30.833332	31
TATACCG	100	2.8789145E-7	18.499998	5
GGTTATC	470	0.0	18.106384	2
TATTCCG	85	2.7255755E-5	17.411764	5
AGGTTAT	535	0.0	16.598131	1
ATACCGT	695	0.0	15.705036	6
TATCTAG	955	0.0	15.6911	1
TACCGTC	670	0.0	15.462687	7
CGAACGT	290	0.0	15.310345	4
CTAGAGT	1150	0.0	15.121739	4
GTGCGAT	175	2.242814E-9	14.8	33
TAGAGTC	1065	0.0	14.765258	5
AGTACTC	1630	0.0	14.41411	5
ACCGTCG	695	0.0	14.374101	8
CAAGACG	840	0.0	14.315475	4
CCGTCGT	690	0.0	14.210145	9
CGTCGTA	710	0.0	14.070422	10
TTATCTA	605	0.0	14.066116	4
TCGAACG	330	0.0	14.015151	3
TCGACTA	80	0.0063018613	13.875	27
>>END_MODULE