FastQCFastQC Report
Thu 9 Feb 2017
SRR2727098.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727098.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2965526
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT304261.0259899930062997No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200580.6763724209465707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT125210.4222185204243699No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC80890.2727677990346401No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG80220.2705085033818621No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT71760.2419806806617106No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA58920.1986831341219062No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG58880.1985482507993523No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG54090.18239597292352183No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT51050.17214484040942482No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44210.14907979225270662No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41020.13832284727903243No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39600.13353448932836873No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG37890.12776822728918916No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA35970.1212938278066016No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC34520.11640430736402244No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA29670.10004970450436111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTTAT5000.017.761
GGTTATC5000.017.762
TATAACG1603.45608E-1117.343752
GTACTAT1554.0199666E-1016.7096776
TAGAGTG3350.016.5671635
CCGATAA3650.015.712339
GTTATCT5950.015.5462183
TAACGAA3600.015.41666613
CGAACGT2650.015.3584914
CGATAAC3650.015.20548110
CGAACGA3700.015.016
TCGTTAC2850.014.92982423
TATCACG1508.114512E-814.82
CGTTTAC1252.9623789E-614.7999991
TTATCTA6150.014.7398384
CGTTACG2650.014.660377524
ACGAACG3950.014.51898915
AGTACTC13900.014.5071955
AACGAAC3850.014.41558414
TATCTAG8350.014.4011971
Produced by FastQC (version 0.11.5)