##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727098.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2965526
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.43778607909693	31.0	30.0	31.0	28.0	34.0
2	30.833750572411102	31.0	31.0	34.0	27.0	34.0
3	31.15130772753299	31.0	31.0	34.0	28.0	34.0
4	35.06494395935156	35.0	35.0	37.0	32.0	37.0
5	34.7749201996543	35.0	35.0	37.0	32.0	37.0
6	34.66325333178667	35.0	35.0	37.0	31.0	37.0
7	34.49889800325474	35.0	35.0	37.0	30.0	37.0
8	34.508303080128115	35.0	35.0	37.0	30.0	37.0
9	35.833446747727045	37.0	35.0	39.0	30.0	39.0
10	35.683914759135476	37.0	35.0	39.0	30.0	39.0
11	35.67499458780668	37.0	35.0	39.0	30.0	39.0
12	35.51792666798403	37.0	35.0	39.0	30.0	39.0
13	35.64528957088894	37.0	35.0	39.0	30.0	39.0
14	36.35586806522688	38.0	35.0	40.0	30.0	41.0
15	36.37460099827147	38.0	35.0	40.0	30.0	41.0
16	36.203019632942016	38.0	34.0	40.0	30.0	41.0
17	36.225159044297705	38.0	34.0	40.0	30.0	41.0
18	36.1202056566019	38.0	34.0	40.0	29.0	41.0
19	36.066976988230756	38.0	34.0	40.0	29.0	41.0
20	36.53590290558909	38.0	35.0	40.0	30.0	41.0
21	36.623850203977305	38.0	35.0	40.0	30.0	41.0
22	36.614331150696366	38.0	35.0	40.0	30.0	41.0
23	36.5266343980798	38.0	35.0	40.0	30.0	41.0
24	36.37882621835047	38.0	35.0	40.0	30.0	41.0
25	36.072557448493114	38.0	34.0	40.0	29.0	41.0
26	35.849711990385515	38.0	34.0	40.0	27.0	41.0
27	35.679187435888274	38.0	34.0	40.0	27.0	41.0
28	35.41039161349454	38.0	34.0	40.0	27.0	41.0
29	35.22762707189214	38.0	34.0	40.0	26.0	41.0
30	34.874417894161105	38.0	33.0	40.0	25.0	41.0
31	34.70280078475117	38.0	33.0	40.0	25.0	41.0
32	34.493635530425294	37.0	33.0	40.0	24.0	41.0
33	34.41202943423865	37.0	33.0	40.0	24.0	41.0
34	34.22659824934936	37.0	33.0	40.0	24.0	41.0
35	33.79634102010908	37.0	32.0	40.0	22.0	41.0
36	33.76803980137082	37.0	32.0	40.0	22.0	41.0
37	33.60512941043174	37.0	32.0	40.0	21.0	41.0
38	33.44693824974052	37.0	31.0	40.0	21.0	41.0
39	33.290428409664926	36.0	31.0	40.0	19.0	41.0
40	33.0239353827955	36.0	31.0	40.0	18.0	41.0
41	32.76503089165295	36.0	31.0	40.0	16.0	41.0
42	32.540270090365084	36.0	30.0	39.0	15.0	41.0
43	32.327173661603375	36.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	7.0
11	14.0
12	26.0
13	28.0
14	58.0
15	164.0
16	274.0
17	563.0
18	969.0
19	2066.0
20	3890.0
21	6782.0
22	11362.0
23	17357.0
24	25620.0
25	35745.0
26	47731.0
27	62707.0
28	79254.0
29	98899.0
30	120585.0
31	145540.0
32	173228.0
33	206067.0
34	243078.0
35	279255.0
36	328974.0
37	381717.0
38	415507.0
39	278054.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.202742447714165	23.018951781235437	14.964663941573942	24.813641829476456
2	18.330879580890542	26.46498462667331	30.915156366863755	24.288979425572393
3	20.01402112137948	27.057796829297736	30.65803503324537	22.27014701607742
4	16.191764968508117	17.96865041817202	31.347322532326476	34.49226208099339
5	13.388215109225143	41.02729161706895	31.613076398588312	13.971416875117601
6	33.44705121452316	36.792427380505174	14.780581927118494	14.979939477853172
7	27.165703487340863	33.67298077980095	20.43222686295787	18.729088869900316
8	28.925829684177444	35.05421972358361	19.42039961881973	16.59955097341922
9	28.764475509572335	14.368614539208222	19.123217938402835	37.74369201281662
10	18.071465230788736	29.87689199150505	30.722340657272944	21.329302120433272
11	34.74446017333856	23.794766931734877	19.71997547821196	21.740797416714607
12	21.17796303252779	30.037436866174836	28.22737011916267	20.557229982134704
13	30.229106067523944	20.659707586445037	25.732972835173257	23.37821351085777
14	22.761223472665556	22.757345577142132	25.773741319415173	28.70768963077714
15	26.329629212490463	28.651713051917266	22.020241940215666	22.9984157953766
16	22.765067647358343	27.061877049804995	26.194138914985064	23.978916387851598
17	23.110031744789964	29.14906158300416	25.393066862337406	22.347839809868468
18	23.38040536484927	25.415929585510295	27.689320545495132	23.514344504145303
19	24.414656961361995	27.339702973435404	26.84963814176642	21.39600192343618
20	23.4784992611766	25.997748797346574	27.70911467307992	22.814637268396908
21	24.040692949581288	27.625014921467557	26.868622969415878	21.465669159535274
22	23.935551399650517	27.25169160546898	25.362448348117674	23.450308646762835
23	22.275474907318298	27.390857473513975	26.663364273319473	23.670303345848257
24	22.28596208564686	29.080878063453163	26.165577371434274	22.4675824794657
25	23.071657439523378	27.902503636791586	25.412388898293255	23.613450025391785
26	23.157814161804684	27.91056291531418	26.420844059367543	22.51077886351359
27	23.525135169949614	28.152004062685677	25.380961084138193	22.941899683226517
28	22.32504452835686	28.6464863231683	26.81729986518412	22.211169283290722
29	22.73879912029097	28.027034664339478	26.98128426457903	22.252881950790517
30	22.44697905194559	29.32977151439576	26.919237936204233	21.304011497454415
31	23.293371900971362	28.422546286898175	25.647625412827267	22.636456399303192
32	21.036976239628316	29.43582352675377	26.21244932602176	23.314750907596157
33	21.632722154518287	28.483041457063603	27.044679426179368	22.83955696223874
34	21.62338148443143	28.79994982340401	26.82704518523864	22.74962350692592
35	22.038720955405548	29.305526237166696	26.96779593232364	21.687956875104113
36	22.297326005572028	29.10458380739201	26.13293560737623	22.46515457965973
37	21.699556840843748	29.25760893682942	26.625158572206075	22.417675650120756
38	22.144300876134622	28.47906239904826	27.03645154350358	22.340185181313537
39	22.116987003317455	27.697784473985394	26.912358886753985	23.27286963594317
40	22.09978937969183	28.47359962448483	27.256783450895387	22.169827544927948
41	20.897338279954383	28.89426698669983	27.440224769568704	22.768169963777083
42	21.967772327742193	29.192426571205242	27.383776099079892	21.456025001972666
43	21.46684938860762	27.96751739826257	27.46575143836203	23.09988177476778
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	768.0
1	1283.0
2	1798.0
3	1824.0
4	1850.0
5	1850.0
6	2409.0
7	2968.0
8	4476.0
9	5984.0
10	9814.0
11	13644.0
12	13644.0
13	29062.0
14	44480.0
15	56579.0
16	68678.0
17	54220.0
18	39762.0
19	39762.0
20	44948.5
21	50135.0
22	45080.5
23	40026.0
24	42543.0
25	45060.0
26	45060.0
27	48902.5
28	52745.0
29	56064.5
30	59384.0
31	63179.0
32	66974.0
33	66974.0
34	76478.0
35	85982.0
36	98617.5
37	111253.0
38	125116.0
39	138979.0
40	138979.0
41	148622.5
42	158266.0
43	169816.5
44	181367.0
45	202992.5
46	224618.0
47	224618.0
48	267074.0
49	309530.0
50	300691.0
51	291852.0
52	250260.5
53	208669.0
54	208669.0
55	192380.0
56	176091.0
57	166657.5
58	157224.0
59	144765.5
60	132307.0
61	132307.0
62	112578.5
63	92850.0
64	80059.5
65	67269.0
66	56795.5
67	46322.0
68	46322.0
69	38016.5
70	29711.0
71	26430.5
72	23150.0
73	18498.0
74	13846.0
75	13846.0
76	10710.0
77	7574.0
78	6527.5
79	5481.0
80	4224.5
81	2968.0
82	2968.0
83	2540.0
84	2112.0
85	1721.0
86	1330.0
87	1343.5
88	1357.0
89	1357.0
90	940.0
91	523.0
92	408.5
93	294.0
94	281.0
95	268.0
96	268.0
97	152.5
98	37.0
99	38.5
100	40.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2965526.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.41315891854346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.05537583348666	43.729655546034905
2	7.700490425807279	7.918130760255039
3	2.505470669170374	3.8644248503941756
4	1.2440926777982495	2.5585093821215072
5	0.7653444478430328	1.967438786218937
6	0.5194359016691658	1.6023504336308239
7	0.34085193168807043	1.22669921720999
8	0.2667444003633324	1.0971337797209273
9	0.21106334288561257	0.9766289870691326
>10	1.208869427162893	11.231963581400752
>50	0.09067701770866265	3.238283706286355
>100	0.07452723623127154	7.925657633962178
>500	0.010141814596821484	3.5356185242933593
>1k	0.006256314199317599	5.450932858116336
>5k	4.6099157258129667E-4	1.5433096918821596
>10k+	1.9756781682055575E-4	2.1332622614035555
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30426	1.0259899930062997	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20058	0.6763724209465707	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12521	0.4222185204243699	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	8089	0.2727677990346401	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	8022	0.2705085033818621	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7176	0.2419806806617106	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	5892	0.1986831341219062	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	5888	0.1985482507993523	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	5409	0.18239597292352183	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	5105	0.17214484040942482	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4421	0.14907979225270662	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4102	0.13832284727903243	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3960	0.13353448932836873	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	3789	0.12776822728918916	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	3597	0.1212938278066016	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	3452	0.11640430736402244	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	2967	0.10004970450436111	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.372083063847695E-5	0.0
3	0.0	0.0	0.0	3.372083063847695E-5	0.0
4	0.0	0.0	0.0	3.372083063847695E-5	0.0
5	0.0	0.0	0.0	3.372083063847695E-5	0.0
6	0.0	0.0	0.0	3.372083063847695E-5	0.0
7	0.0	0.0	0.0	3.372083063847695E-5	0.0
8	0.0	0.0	0.0	3.372083063847695E-5	0.0
9	0.0	0.0	0.0	3.372083063847695E-5	3.372083063847695E-5
10	0.0	0.0	0.0	1.0116249191543085E-4	3.372083063847695E-5
11	3.372083063847695E-5	0.0	0.0	2.3604581446933867E-4	3.372083063847695E-5
12	3.372083063847695E-5	0.0	0.0	2.697666451078156E-4	3.372083063847695E-5
13	3.372083063847695E-5	0.0	0.0	3.034874757462926E-4	3.372083063847695E-5
14	3.372083063847695E-5	0.0	0.0	5.058124595771542E-4	1.348833225539078E-4
15	3.372083063847695E-5	0.0	0.0	9.104624272388776E-4	1.348833225539078E-4
16	6.74416612769539E-5	0.0	0.0	0.002056970668947094	1.6860415319238476E-4
17	6.74416612769539E-5	0.0	0.0	0.003304641402570741	1.6860415319238476E-4
18	6.74416612769539E-5	0.0	0.0	0.004012778845978757	1.6860415319238476E-4
19	6.74416612769539E-5	0.0	0.0	0.006373236990672144	1.6860415319238476E-4
20	6.74416612769539E-5	0.0	0.0	0.008025557691957515	1.6860415319238476E-4
21	6.74416612769539E-5	0.0	0.0	0.013859261392414028	2.023249838308617E-4
22	6.74416612769539E-5	0.0	0.0	0.022829002342248897	2.023249838308617E-4
23	6.74416612769539E-5	0.0	0.0	0.04147662168532665	2.3604581446933867E-4
24	6.74416612769539E-5	0.0	0.0	0.07846837289573587	2.697666451078156E-4
25	6.74416612769539E-5	0.0	0.0	0.10160086271373106	2.697666451078156E-4
26	6.74416612769539E-5	0.0	0.0	0.13262402690112984	2.697666451078156E-4
27	6.74416612769539E-5	0.0	0.0	0.17892272736775872	2.697666451078156E-4
28	6.74416612769539E-5	0.0	0.0	0.2526701839741078	2.697666451078156E-4
29	6.74416612769539E-5	0.0	0.0	0.32044905355744646	2.697666451078156E-4
30	6.74416612769539E-5	0.0	0.0	0.4206336413843615	2.697666451078156E-4
31	6.74416612769539E-5	0.0	0.0	0.6113923803062256	2.697666451078156E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTTAT	500	0.0	17.76	1
GGTTATC	500	0.0	17.76	2
TATAACG	160	3.45608E-11	17.34375	2
GTACTAT	155	4.0199666E-10	16.709677	6
TAGAGTG	335	0.0	16.567163	5
CCGATAA	365	0.0	15.71233	9
GTTATCT	595	0.0	15.546218	3
TAACGAA	360	0.0	15.416666	13
CGAACGT	265	0.0	15.358491	4
CGATAAC	365	0.0	15.205481	10
CGAACGA	370	0.0	15.0	16
TCGTTAC	285	0.0	14.929824	23
TATCACG	150	8.114512E-8	14.8	2
CGTTTAC	125	2.9623789E-6	14.799999	1
TTATCTA	615	0.0	14.739838	4
CGTTACG	265	0.0	14.6603775	24
ACGAACG	395	0.0	14.518989	15
AGTACTC	1390	0.0	14.507195	5
AACGAAC	385	0.0	14.415584	14
TATCTAG	835	0.0	14.401197	1
>>END_MODULE