FastQCFastQC Report
Thu 9 Feb 2017
SRR2727097.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727097.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3704972
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT276140.7453227716700693No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178090.480678396489906No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG152270.41098826117984155No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC143600.38758727461368125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT118150.31889579732316464No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA100310.2707442863265903No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG86930.2346306530791596No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT80560.21743754068856658No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG75640.20415808810430955No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG75440.20361827295860804No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC69760.18828752282068528No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64920.17522399629470883No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG59380.16027111675877712No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC55120.14877305415533504No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA47640.1285839677060987No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT46200.12469729865704787No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC42030.11344215286917148No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40450.10917761321812959No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC38810.10475112902337724No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37630.10156621966373836No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT37450.101080386032607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT1501.2732926E-1118.56
AGGTTAT5550.017.01
GGTTATC5350.016.5981312
CGATAAC4600.016.08695610
TTATCTA6100.015.7704934
CCGATAA4850.015.6391759
AAGACGG10200.015.5980415
TCTATAC3000.015.4166673
TAGAGTG5700.015.2543865
ACGAACG4550.015.04395715
TATCTAG9150.014.9617491
CGAACGA4650.014.72043116
CAAGACG11000.014.6318184
ACCGTCG6800.014.4191188
CGTCGTA6550.014.4045810
AATTCCG5700.014.2807025
GTTATCT6100.014.2540993
GTATTAG7050.014.1702131
TCCGATA5500.014.1272728
ATTCCGA5500.014.1272726
Produced by FastQC (version 0.11.5)