FastQCFastQC Report
Thu 9 Feb 2017
SRR2727096.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727096.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492750
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT167510.671988767425534No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG118860.4768227860796309No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC113710.45616287232975633No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113010.45335472871326843No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA85600.34339584795908135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT72530.290963795005516No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG67990.27275097783572355No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT64120.2572259552702838No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG59460.23853174205195066No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG58720.23556313308594926No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC54040.21678868719285932No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG47590.190913649583793No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC45720.1834118944940327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41210.16531942633637547No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA38830.1557717380403169No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT35350.14181125263263464No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC33560.13463042824190152No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC32920.13206298264968408No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25430.10201584595326448No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT25090.10065189048239895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC656.906269E-519.9230777
GCGTCGA1002.878278E-718.49999821
TAGAGTG4400.017.2386365
AAGACGG7200.017.2152795
AGGTTAT3800.017.0394741
TCGAACG1901.8189894E-1216.5526313
CAAGACG8000.016.4187494
GGTTATC3750.016.2799992
CGAACGT1951.8189894E-1216.1282064
GTATTAG5450.015.6146781
GTCGAAC957.0649665E-515.57894823
ACCGTCC1455.3532858E-815.3103458
CGTCGAA1101.4531435E-515.13636422
CAAATCG4100.014.890244513
ATTAGAG4600.014.8804353
AGAGTGT5500.014.86
TTAGAGT4250.014.7999994
GGTATAC908.278797E-414.3888891
AATTAGA4400.014.2954542
AGTACTC11100.014.1666675
Produced by FastQC (version 0.11.5)