FastQCFastQC Report
Thu 9 Feb 2017
SRR2727095.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727095.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2505939
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT101960.4068734314761852No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT97140.38763912449584764No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT73310.29254503002666865No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46450.18535965959267164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48850.015.5649941
CTTATAC17750.015.52957737
TCTTATA29300.014.83788537
CTAGTAC1501.307144E-613.5666673
GTATAGT2951.8189894E-1212.5423721
GTACTAG1951.4693978E-712.3333341
GTGCGAT1401.3995683E-411.89285811
ATACCGT1257.2239223E-411.846
TAAGCGT1100.00374716211.7727274
TTTTACG1100.00374716211.7727272
CTCTTAT42700.011.69789237
CTATCCT3650.011.6575354
GTATCAA65400.011.6261472
TCTACAC5450.011.5412853
TATACAC7400.011.537
TCTCTTA61450.011.47030137
TAGTACT2103.846144E-711.452384
TATACTG3800.011.1973695
GTACTAC2207.0158603E-710.9318181
TACTAGG2207.0158603E-710.9318182
Produced by FastQC (version 0.11.5)