##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2727095.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2505939 Sequences flagged as poor quality 0 Sequence length 43 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.483550078433673 31.0 31.0 34.0 30.0 34.0 2 31.734614050860774 31.0 31.0 34.0 30.0 34.0 3 31.84845720506365 31.0 31.0 34.0 30.0 34.0 4 35.60160841903973 37.0 35.0 37.0 33.0 37.0 5 35.1677415132611 37.0 35.0 37.0 32.0 37.0 6 35.179553053765474 37.0 35.0 37.0 32.0 37.0 7 35.131936970532806 37.0 35.0 37.0 32.0 37.0 8 35.08056022113866 37.0 35.0 37.0 32.0 37.0 9 36.9049418202119 39.0 37.0 39.0 33.0 39.0 10 36.651812753622494 38.0 35.0 39.0 32.0 39.0 11 36.78042402468695 39.0 37.0 39.0 32.0 39.0 12 36.6461154880466 38.0 35.0 39.0 32.0 39.0 13 36.7949590951735 39.0 37.0 39.0 33.0 39.0 14 37.79628354880147 39.0 37.0 41.0 33.0 41.0 15 37.793364882385404 39.0 37.0 41.0 33.0 41.0 16 37.73993979901346 39.0 37.0 41.0 33.0 41.0 17 37.645432710053996 39.0 37.0 40.0 32.0 41.0 18 37.670723030369054 39.0 37.0 40.0 32.0 41.0 19 37.71336931984378 39.0 37.0 40.0 32.0 41.0 20 37.589466463469385 39.0 37.0 40.0 32.0 41.0 21 37.58317780281164 39.0 37.0 40.0 32.0 41.0 22 37.48322924061599 39.0 36.0 40.0 32.0 41.0 23 37.4197568256849 39.0 36.0 40.0 32.0 41.0 24 37.30891294640452 39.0 36.0 40.0 32.0 41.0 25 37.280900692315335 39.0 36.0 40.0 32.0 41.0 26 37.131965303225655 39.0 36.0 40.0 31.0 41.0 27 36.93241295977276 39.0 36.0 40.0 31.0 41.0 28 36.81788942188936 39.0 36.0 40.0 30.0 41.0 29 36.73036294977651 39.0 36.0 40.0 30.0 41.0 30 36.669979596470625 39.0 35.0 40.0 30.0 41.0 31 36.61961803539511 39.0 35.0 40.0 30.0 41.0 32 36.514526889920305 38.0 35.0 40.0 30.0 41.0 33 36.486826694504536 38.0 35.0 40.0 30.0 41.0 34 36.47249035191998 38.0 35.0 40.0 30.0 41.0 35 36.36229652836721 38.0 35.0 40.0 30.0 41.0 36 36.27494284577558 38.0 35.0 40.0 30.0 41.0 37 36.124485472311974 38.0 35.0 40.0 30.0 41.0 38 36.07494954984938 38.0 35.0 40.0 29.0 41.0 39 35.95542030352694 38.0 35.0 40.0 29.0 41.0 40 35.88546050003612 38.0 35.0 40.0 29.0 41.0 41 35.817569382175705 38.0 35.0 40.0 28.0 41.0 42 35.75002783387784 38.0 34.0 40.0 28.0 41.0 43 34.40408764938013 37.0 33.0 40.0 24.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 3.0 10 3.0 11 16.0 12 5.0 13 5.0 14 9.0 15 7.0 16 18.0 17 28.0 18 79.0 19 175.0 20 423.0 21 841.0 22 1693.0 23 3092.0 24 5350.0 25 8810.0 26 13782.0 27 20560.0 28 29906.0 29 42549.0 30 57486.0 31 76283.0 32 98254.0 33 127057.0 34 165624.0 35 218097.0 36 296599.0 37 414961.0 38 590762.0 39 333461.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.5167172864144 18.626351239994268 12.912365384791888 25.944566088799448 2 18.516891273091645 20.822773419464717 35.42604189487454 25.2342934125691 3 20.051964553007874 22.508608549529736 29.988279842406378 27.45114705505601 4 15.23001956552015 15.609677649775195 33.72073302662196 35.4395697580827 5 15.644355269621487 35.46323354239668 33.62751447660936 15.264896711372463 6 35.55888630968272 33.89643562752326 14.98747575260212 15.557202310191908 7 29.21763059675435 30.177669927320654 21.223461544754283 19.381237931170713 8 26.552242492734262 34.696854153273485 19.47166311709902 19.279240236893237 9 26.622994414468987 14.759257906916329 19.60570468794332 39.01204299067136 10 17.431669326348327 26.471873417509368 32.84609082663225 23.250366429510056 11 35.61746714505022 21.75348242714607 21.280605792878436 21.348444634925272 12 21.401957509739862 24.81377240228114 30.225715789570295 23.5585542984087 13 30.95969215531583 19.677933102122598 24.312403454353838 25.049971288207733 14 22.303655436145892 20.564786293680733 25.790372391347116 31.34118587882626 15 25.280902687575395 27.365510493272183 22.705820053880004 24.64776676527242 16 24.333034443376313 26.029484356961603 24.86517030143192 24.772310898230167 17 23.969099008395656 26.130484421208976 25.660919918641277 24.23949665175409 18 24.061718980390186 25.320807888779417 26.39322026593624 24.224252864894158 19 24.96613046047809 25.076867393819242 26.84171482226822 23.11528732343445 20 24.471505491554264 25.00048883871475 26.62123858561601 23.90676708411498 21 24.007088759941883 25.353091196553468 26.970608622157204 23.66921142134745 22 24.641501648683388 24.96517273564919 26.21392619692658 24.17939941874084 23 24.005532457094926 25.238882510707562 26.417801869877916 24.337783162319592 24 23.99994572892636 25.199615792722806 26.463852472067355 24.336586006283472 25 24.40653982399412 24.970599843012938 26.289187406397364 24.33367292659558 26 23.989330945406092 25.33178181911052 26.554197847593258 24.12468938789013 27 24.280519198591826 25.62991357730575 26.202433498979822 23.8871337251226 28 24.03414448635821 25.73115307276035 26.14504981964844 24.089652621233 29 23.938611434675785 25.88159568129951 26.49394099377519 23.685851890249523 30 24.23550613163369 25.66650664680984 26.247406660736754 23.85058056081972 31 24.095638401413602 25.17511399918354 26.62455071731594 24.104696882086916 32 23.61334413966182 25.59008818650414 26.481450665798327 24.31511700803571 33 23.692835300460228 25.20979161902983 26.477420240476725 24.619952840033214 34 24.10625318493387 25.10683619992346 26.823996912933634 23.962913702209033 35 23.828433174151485 25.1350092719735 26.76266261868306 24.273894935191958 36 23.62763020169286 24.92881909735233 27.032820830834268 24.410729870120544 37 23.446221157019384 24.558019967764576 27.494484103563575 24.50127477165246 38 23.391590936571085 24.431720005953856 27.66244509543129 24.514243962043768 39 22.895609190806322 24.474897433656608 28.304839024413603 24.32465435112347 40 23.260821592225508 24.32166146103317 28.228380658906698 24.18913628783462 41 22.684071719223812 24.454146729030516 28.403165440180306 24.458616111565366 42 22.374447263081823 24.79601458774535 28.244342739388312 24.585195409784514 43 22.271052886762206 24.461688812058075 28.16333518094415 25.103923120235567 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1165.0 1 1025.5 2 886.0 3 1745.5 4 2605.0 5 2605.0 6 3289.0 7 3973.0 8 3820.5 9 3668.0 10 5131.5 11 6595.0 12 6595.0 13 10654.5 14 14714.0 15 19077.5 16 23441.0 17 21348.0 18 19255.0 19 19255.0 20 21207.5 21 23160.0 22 19839.0 23 16518.0 24 19456.5 25 22395.0 26 22395.0 27 26620.0 28 30845.0 29 35519.5 30 40194.0 31 46633.5 32 53073.0 33 53073.0 34 60954.0 35 68835.0 36 78682.0 37 88529.0 38 100047.0 39 111565.0 40 111565.0 41 124217.0 42 136869.0 43 150801.5 44 164734.0 45 178037.0 46 191340.0 47 191340.0 48 203622.5 49 215905.0 50 220039.0 51 224173.0 52 222748.5 53 221324.0 54 221324.0 55 210935.0 56 200546.0 57 186301.5 58 172057.0 59 157036.5 60 142016.0 61 142016.0 62 124240.0 63 106464.0 64 90333.5 65 74203.0 66 61380.0 67 48557.0 68 48557.0 69 39660.5 70 30764.0 71 24708.5 72 18653.0 73 15192.0 74 11731.0 75 11731.0 76 9421.5 77 7112.0 78 5623.0 79 4134.0 80 3121.0 81 2108.0 82 2108.0 83 1586.0 84 1064.0 85 782.5 86 501.0 87 346.5 88 192.0 89 192.0 90 120.5 91 49.0 92 34.5 93 20.0 94 12.5 95 5.0 96 5.0 97 3.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 2505939.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.47380461316963 #Duplication Level Percentage of deduplicated Percentage of total 1 82.93129014966921 52.639645072781825 2 9.03977418162422 11.47577720303182 3 2.9752281761326564 5.6654715579432375 4 1.507475166792006 3.8274073678464435 5 0.8487928449417594 2.6938055598444812 6 0.5680094084070975 2.1632230924604507 7 0.4041195701069729 1.7955704643329677 8 0.2968998291354133 1.5076289395379738 9 0.22490545369361478 1.2848044341766296 >10 1.1501004530625039 12.549966550455677 >50 0.04210897784587247 1.7723482380333964 >100 0.010603947868162293 1.047567986833819 >500 1.2578922190792274E-4 0.04858459020493172 >1k 3.773676657233015E-4 0.4406945696561879 >5k 1.2578922190776718E-4 0.6804637140854899 >10k+ 6.289461095388359E-5 0.4070406587747524 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 10196 0.4068734314761852 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 9714 0.38763912449584764 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 7331 0.29254503002666865 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4645 0.18535965959267164 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.990520120401973E-5 0.0 4 0.0 0.0 0.0 3.990520120401973E-5 0.0 5 0.0 0.0 0.0 3.990520120401973E-5 0.0 6 0.0 0.0 0.0 3.990520120401973E-5 0.0 7 0.0 0.0 0.0 3.990520120401973E-5 0.0 8 0.0 0.0 0.0 3.990520120401973E-5 7.981040240803946E-5 9 0.0 0.0 0.0 3.990520120401973E-5 1.5962080481607893E-4 10 3.990520120401973E-5 0.0 0.0 7.981040240803946E-5 1.5962080481607893E-4 11 3.990520120401973E-5 0.0 0.0 7.981040240803946E-5 1.5962080481607893E-4 12 3.990520120401973E-5 0.0 0.0 7.981040240803946E-5 1.5962080481607893E-4 13 3.990520120401973E-5 3.990520120401973E-5 0.0 7.981040240803946E-5 1.5962080481607893E-4 14 3.990520120401973E-5 3.990520120401973E-5 0.0 7.981040240803946E-5 1.9952600602009865E-4 15 3.990520120401973E-5 3.990520120401973E-5 0.0 1.5962080481607893E-4 2.394312072241184E-4 16 3.990520120401973E-5 3.990520120401973E-5 0.0 1.9952600602009865E-4 2.394312072241184E-4 17 3.990520120401973E-5 3.990520120401973E-5 0.0 1.9952600602009865E-4 2.394312072241184E-4 18 3.990520120401973E-5 3.990520120401973E-5 0.0 3.5914681083617757E-4 2.394312072241184E-4 19 7.981040240803946E-5 3.990520120401973E-5 0.0 6.783884204683354E-4 2.394312072241184E-4 20 7.981040240803946E-5 3.990520120401973E-5 0.0 8.779144264884341E-4 2.394312072241184E-4 21 7.981040240803946E-5 3.990520120401973E-5 0.0 0.0014764924445487301 2.394312072241184E-4 22 7.981040240803946E-5 3.990520120401973E-5 0.0 0.0021548808650170654 2.394312072241184E-4 23 7.981040240803946E-5 3.990520120401973E-5 0.0 0.002633743279465302 3.1924160963215785E-4 24 7.981040240803946E-5 3.990520120401973E-5 0.0 0.0035914681083617757 3.1924160963215785E-4 25 7.981040240803946E-5 3.990520120401973E-5 0.0 0.004549192937258249 3.1924160963215785E-4 26 1.197156036120592E-4 3.990520120401973E-5 0.0 0.006903599808295413 3.1924160963215785E-4 27 1.197156036120592E-4 3.990520120401973E-5 0.0 0.024022931124819877 3.5914681083617757E-4 28 1.197156036120592E-4 3.990520120401973E-5 0.0 0.07478234705633298 3.5914681083617757E-4 29 1.197156036120592E-4 3.990520120401973E-5 0.0 0.16029919323654726 3.5914681083617757E-4 30 1.197156036120592E-4 3.990520120401973E-5 0.0 0.2620973615080016 3.5914681083617757E-4 31 1.197156036120592E-4 3.990520120401973E-5 0.0 0.5595906364839687 3.5914681083617757E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4885 0.0 15.564994 1 CTTATAC 1775 0.0 15.529577 37 TCTTATA 2930 0.0 14.837885 37 CTAGTAC 150 1.307144E-6 13.566667 3 GTATAGT 295 1.8189894E-12 12.542372 1 GTACTAG 195 1.4693978E-7 12.333334 1 GTGCGAT 140 1.3995683E-4 11.892858 11 ATACCGT 125 7.2239223E-4 11.84 6 TAAGCGT 110 0.003747162 11.772727 4 TTTTACG 110 0.003747162 11.772727 2 CTCTTAT 4270 0.0 11.697892 37 CTATCCT 365 0.0 11.657535 4 GTATCAA 6540 0.0 11.626147 2 TCTACAC 545 0.0 11.541285 3 TATACAC 740 0.0 11.5 37 TCTCTTA 6145 0.0 11.470301 37 TAGTACT 210 3.846144E-7 11.45238 4 TATACTG 380 0.0 11.197369 5 GTACTAC 220 7.0158603E-7 10.931818 1 TACTAGG 220 7.0158603E-7 10.931818 2 >>END_MODULE