FastQCFastQC Report
Thu 9 Feb 2017
SRR2727094.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727094.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3560636
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT87330.2452651717277475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT82480.23164400966568893No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63460.1782265864862345No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43990.12354534414638285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34850.018.048781
CTTATAC22100.016.5746637
TCTTATA37950.014.77075137
GTGTACG2650.013.9622641
TCGTTAC4250.013.92941223
TAGTACT2703.6379788E-1213.0185184
CGTTACG3900.012.80769324
GTATCAA50150.012.6161522
TATACTG5150.012.2135935
GTACTAG2451.5643309E-912.0816341
CTCTTAT55300.011.94303837
GTATATA4500.011.9222221
GGGTAAG14000.011.49642851
TCTATAG2901.5643309E-1011.4827593
CTCGTTA5350.011.41121522
TAGACAG7700.011.2922085
GTAGTAC3451.8189894E-1211.260873
TCTCTTA79700.011.14178237
GTGTAAG5650.011.1327441
GTAAGAG15400.011.0519483
Produced by FastQC (version 0.11.5)