##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727094.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3560636
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63947732933105	31.0	31.0	34.0	30.0	34.0
2	31.889706221023435	31.0	31.0	34.0	30.0	34.0
3	32.0090677620515	33.0	31.0	34.0	30.0	34.0
4	35.73264018001279	37.0	35.0	37.0	35.0	37.0
5	35.33634075485391	37.0	35.0	37.0	33.0	37.0
6	35.340707671326136	37.0	35.0	37.0	33.0	37.0
7	35.29488130772143	37.0	35.0	37.0	32.0	37.0
8	35.23730732374778	37.0	35.0	37.0	32.0	37.0
9	37.07833881362768	39.0	37.0	39.0	33.0	39.0
10	36.84144040559046	39.0	37.0	39.0	33.0	39.0
11	36.959133143629394	39.0	37.0	39.0	33.0	39.0
12	36.834977515252895	39.0	37.0	39.0	33.0	39.0
13	36.971569966713815	39.0	37.0	39.0	33.0	39.0
14	38.02842722479917	40.0	37.0	41.0	33.0	41.0
15	38.029686550380326	40.0	37.0	41.0	33.0	41.0
16	37.98076888510929	40.0	37.0	41.0	33.0	41.0
17	37.889471431508305	39.0	37.0	41.0	33.0	41.0
18	37.903664120679565	39.0	37.0	41.0	33.0	41.0
19	37.94382099153073	40.0	37.0	41.0	33.0	41.0
20	37.81860150826987	39.0	37.0	41.0	33.0	41.0
21	37.81330020816506	39.0	37.0	41.0	33.0	41.0
22	37.7185418560055	39.0	37.0	41.0	32.0	41.0
23	37.659656589440765	39.0	37.0	41.0	32.0	41.0
24	37.56473085145463	39.0	37.0	41.0	32.0	41.0
25	37.53978165698488	39.0	37.0	41.0	32.0	41.0
26	37.40579772827102	39.0	36.0	40.0	32.0	41.0
27	37.221836492132304	39.0	36.0	40.0	31.0	41.0
28	37.12386186063389	39.0	36.0	40.0	31.0	41.0
29	37.04318217307245	39.0	36.0	40.0	31.0	41.0
30	36.9957822141887	39.0	36.0	40.0	31.0	41.0
31	36.95967040719692	39.0	36.0	40.0	31.0	41.0
32	36.86232178745595	39.0	36.0	40.0	30.0	41.0
33	36.84322435654754	39.0	36.0	40.0	30.0	41.0
34	36.83871589232935	39.0	36.0	40.0	30.0	41.0
35	36.74046265891824	39.0	35.0	40.0	30.0	41.0
36	36.673786087654	39.0	35.0	40.0	30.0	41.0
37	36.54692560542555	39.0	35.0	40.0	30.0	41.0
38	36.50481599354722	38.0	35.0	40.0	30.0	41.0
39	36.41203734389025	38.0	35.0	40.0	30.0	41.0
40	36.35344387912721	38.0	35.0	40.0	30.0	41.0
41	36.302757428729024	38.0	35.0	40.0	30.0	41.0
42	36.25825442420961	38.0	35.0	40.0	30.0	41.0
43	34.90325660921251	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	5.0
12	2.0
13	1.0
14	5.0
15	6.0
16	13.0
17	29.0
18	55.0
19	171.0
20	397.0
21	865.0
22	1814.0
23	3326.0
24	5796.0
25	9799.0
26	15617.0
27	23709.0
28	35511.0
29	50965.0
30	70257.0
31	93822.0
32	124752.0
33	164191.0
34	217440.0
35	292894.0
36	404204.0
37	582607.0
38	880788.0
39	581586.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.4646888926585	18.405812894100944	12.504535706542313	26.624962506698242
2	18.440216860133976	20.41157815626197	35.625714057825626	25.522490925778428
3	19.905994322362634	22.127226708936266	29.722751778053137	28.244027190647962
4	15.084327631355746	15.328216644442172	33.85836125905597	35.72909446514612
5	15.673997566726843	35.29203771461054	33.79517030103611	15.238794417626513
6	35.815034168053124	33.87223518495011	15.105672132731343	15.207058514265428
7	29.6158045922133	29.90114687376075	21.161135257858426	19.321913276167514
8	26.47678673135923	34.86747873132777	19.514378891860893	19.141355645452105
9	26.833548837904242	14.682068035036439	19.368337566659438	39.11604556039988
10	17.204117466654832	26.259859193694606	33.07889377066344	23.457129568987114
11	36.11453122419703	21.491272907424406	21.279653410233454	21.11454245814512
12	21.579599824301052	24.40774625656765	30.225555209799598	23.7870987093317
13	30.896137656306344	19.584029370033893	24.22541927902768	25.294413694632084
14	22.372126777351014	20.271153805106728	25.398917496761815	31.957801920780444
15	25.300030668678293	27.269117090317575	22.409872842941542	25.020979398062593
16	24.75970585030315	25.90295666279844	24.420412533041848	24.916924953856558
17	24.31545937298842	25.904276651699305	25.296800908601725	24.483463066710552
18	24.3915132015741	25.17755254960069	25.9834198160104	24.447514432814813
19	25.27590576514982	24.956215687309797	26.15411965727471	23.61375889026567
20	24.705810984329766	24.843370678721442	26.088766164247062	24.36205217270173
21	24.404460326750616	25.154635295492156	26.36997996987055	24.070924407886682
22	24.96770239923429	24.72920568123223	25.667717789743183	24.635374129790296
23	24.405583721559857	24.977981461738857	25.852965593787175	24.763469222914107
24	24.327367357966388	24.944644720774605	25.933091728556356	24.79489619270265
25	24.77419764334237	24.734373297354743	25.735598921091622	24.755830138211262
26	24.40269097992606	25.08914137811335	25.887481899301136	24.620685742659457
27	24.681770335411986	25.378303202012226	25.593966920516447	24.34595954205934
28	24.48071074942791	25.307894432342987	25.548441345871918	24.662953472357184
29	24.360226656136714	25.531197235550053	25.936405743243622	24.17217036506961
30	24.764367938761502	25.22877935290212	25.60775659179989	24.399096116536484
31	24.567352574090695	24.836405630904142	25.995412055599054	24.600829739406105
32	24.070952492756913	25.303709786678557	25.917925898631594	24.707411821932936
33	24.163660649389605	24.78537542169433	26.006786428042634	25.044177500873438
34	24.547019128043416	24.72760484362906	26.34206360886089	24.383312419466634
35	24.32590694471437	24.797367661282983	26.222730995249165	24.653994398753483
36	24.144647192243184	24.52081594411785	26.493862332459706	24.84067453117926
37	23.96080363171074	24.18427494413919	26.88123694755656	24.973684476593508
38	23.868825681704056	24.08375919358227	27.121250248551103	24.926164876162574
39	23.435981661703135	24.07673797602451	27.709740619372493	24.777539742899865
40	23.807656834340833	23.836780844770427	27.734146371603273	24.621415949285463
41	23.191643290693012	24.05134925333564	27.89088803236276	24.86611942360859
42	22.885574374915045	24.306640723735875	27.820956705487447	24.986828195861637
43	22.74121814192745	23.965858908352327	27.770825212125022	25.522097737595196
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1214.0
1	1121.5
2	1029.0
3	2060.0
4	3091.0
5	3091.0
6	3675.0
7	4259.0
8	3973.5
9	3688.0
10	4946.5
11	6205.0
12	6205.0
13	10006.5
14	13808.0
15	16957.5
16	20107.0
17	19062.5
18	18018.0
19	18018.0
20	20879.5
21	23741.0
22	22019.0
23	20297.0
24	24598.0
25	28899.0
26	28899.0
27	35160.0
28	41421.0
29	48006.5
30	54592.0
31	64164.5
32	73737.0
33	73737.0
34	85492.5
35	97248.0
36	112219.5
37	127191.0
38	143998.5
39	160806.0
40	160806.0
41	179279.0
42	197752.0
43	216878.5
44	236005.0
45	254548.5
46	273092.0
47	273092.0
48	290482.0
49	307872.0
50	316497.0
51	325122.0
52	322859.0
53	320596.0
54	320596.0
55	304988.0
56	289380.0
57	270676.5
58	251973.0
59	229824.0
60	207675.0
61	207675.0
62	181577.5
63	155480.0
64	131743.0
65	108006.0
66	89896.5
67	71787.0
68	71787.0
69	58649.0
70	45511.0
71	36905.0
72	28299.0
73	22992.0
74	17685.0
75	17685.0
76	14256.0
77	10827.0
78	8749.0
79	6671.0
80	5209.5
81	3748.0
82	3748.0
83	2906.5
84	2065.0
85	1596.0
86	1127.0
87	794.0
88	461.0
89	461.0
90	287.0
91	113.0
92	67.0
93	21.0
94	15.5
95	10.0
96	10.0
97	7.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3560636.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.84462706882534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.45740502686945	50.99547462949825
2	9.270591033467875	11.46672490344834
3	3.0892956602177337	5.731690140345188
4	1.4952522328126998	3.6989326684851935
5	0.8670952102304149	2.681258995493235
6	0.5631896317244492	2.089815164581659
7	0.396685599536467	1.717301107683434
8	0.29862639492206644	1.4774750421490364
9	0.22876727935656566	1.273322436962093
>10	1.257227575113503	13.816252717412873
>50	0.05879928436680052	2.4493844784622203
>100	0.01642870844708538	1.5551987917516983
>500	2.2727248363252955E-4	0.07966703850909648
>1k	2.72726967474973E-4	0.3117527938827899
>5k	1.3636348373748623E-4	0.6557490913348812
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8733	0.2452651717277475	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8248	0.23164400966568893	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6346	0.1782265864862345	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4399	0.12354534414638285	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	2.808487023104861E-5
9	0.0	0.0	0.0	0.0	1.6850922138629166E-4
10	0.0	0.0	0.0	0.0	1.6850922138629166E-4
11	0.0	0.0	0.0	0.0	1.6850922138629166E-4
12	0.0	0.0	0.0	0.0	1.6850922138629166E-4
13	0.0	2.808487023104861E-5	0.0	0.0	1.6850922138629166E-4
14	0.0	2.808487023104861E-5	0.0	0.0	3.089335725415347E-4
15	0.0	2.808487023104861E-5	0.0	0.0	3.6510331300363194E-4
16	2.808487023104861E-5	2.808487023104861E-5	0.0	5.616974046209722E-5	5.05527664158875E-4
17	2.808487023104861E-5	2.808487023104861E-5	0.0	1.6850922138629166E-4	5.336125343899236E-4
18	2.808487023104861E-5	2.808487023104861E-5	0.0	2.527638320794375E-4	5.897822748520208E-4
19	2.808487023104861E-5	2.808487023104861E-5	0.0	4.2127305346572913E-4	5.897822748520208E-4
20	2.808487023104861E-5	2.808487023104861E-5	0.0	5.897822748520208E-4	5.897822748520208E-4
21	5.616974046209722E-5	2.808487023104861E-5	0.0	8.425461069314583E-4	5.897822748520208E-4
22	5.616974046209722E-5	2.808487023104861E-5	0.0	0.001432328381783479	5.897822748520208E-4
23	5.616974046209722E-5	2.808487023104861E-5	0.0	0.001937856045942354	6.740368855451666E-4
24	5.616974046209722E-5	2.808487023104861E-5	0.0	0.0024995534505633265	6.740368855451666E-4
25	5.616974046209722E-5	2.808487023104861E-5	0.0	0.0031455054658774445	6.740368855451666E-4
26	5.616974046209722E-5	2.808487023104861E-5	0.0	0.005645058916440771	6.740368855451666E-4
27	5.616974046209722E-5	2.808487023104861E-5	0.0	0.020333446047279193	6.740368855451666E-4
28	5.616974046209722E-5	2.808487023104861E-5	0.0	0.06709475498197513	6.740368855451666E-4
29	5.616974046209722E-5	2.808487023104861E-5	0.0	0.13730693055959667	6.740368855451666E-4
30	5.616974046209722E-5	2.808487023104861E-5	0.0	0.22465087697815783	6.740368855451666E-4
31	8.425461069314583E-5	2.808487023104861E-5	0.0	0.5048817121435609	7.302066260072639E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3485	0.0	18.04878	1
CTTATAC	2210	0.0	16.57466	37
TCTTATA	3795	0.0	14.770751	37
GTGTACG	265	0.0	13.962264	1
TCGTTAC	425	0.0	13.929412	23
TAGTACT	270	3.6379788E-12	13.018518	4
CGTTACG	390	0.0	12.807693	24
GTATCAA	5015	0.0	12.616152	2
TATACTG	515	0.0	12.213593	5
GTACTAG	245	1.5643309E-9	12.081634	1
CTCTTAT	5530	0.0	11.943038	37
GTATATA	450	0.0	11.922222	1
GGGTAAG	1400	0.0	11.4964285	1
TCTATAG	290	1.5643309E-10	11.482759	3
CTCGTTA	535	0.0	11.411215	22
TAGACAG	770	0.0	11.292208	5
GTAGTAC	345	1.8189894E-12	11.26087	3
TCTCTTA	7970	0.0	11.141782	37
GTGTAAG	565	0.0	11.132744	1
GTAAGAG	1540	0.0	11.051948	3
>>END_MODULE