FastQCFastQC Report
Thu 9 Feb 2017
SRR2727093.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727093.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2713024
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT92730.3417957231487816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT83480.3077009270835791No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61680.22734778608666933No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46510.1714323205397372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36950.018.1244931
GTATACG1005.883723E-616.651
TCTTATA27000.015.07407437
CTTATAC16350.015.04892937
CTCTTAT42100.013.44655537
GTATCAA50700.013.0631162
TACCCGT1451.3734916E-512.758626
TTAGTAC1653.80834E-612.3333333
GTATAGG3250.011.9538471
GTCTATA1901.4356665E-611.6842111
TAGACTG3205.456968E-1211.5625015
TATTCCG1451.9400632E-411.48275855
GATATAA2301.0246367E-711.260871
TATAGGG2301.0246367E-711.260872
GTATTAC2002.6605321E-611.0999991
TAGTACT2553.637615E-810.8823534
GTAAGAG12100.010.8553713
AGGCCGT2901.895387E-910.8448286
CTTACAC4650.010.7419363
ACCCGAC10350.010.72463811
Produced by FastQC (version 0.11.5)