##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727093.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2713024
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.097212925503054	31.0	31.0	34.0	28.0	34.0
2	31.34827557736312	31.0	31.0	34.0	28.0	34.0
3	31.441802947559623	31.0	31.0	34.0	28.0	34.0
4	35.259301797551366	37.0	35.0	37.0	33.0	37.0
5	34.70688722252365	35.0	35.0	37.0	32.0	37.0
6	34.72955860324125	35.0	35.0	37.0	32.0	37.0
7	34.68413217133354	35.0	35.0	37.0	32.0	37.0
8	34.62001957962775	35.0	35.0	37.0	31.0	37.0
9	36.39159476657781	38.0	35.0	39.0	32.0	39.0
10	36.09038143414876	37.0	35.0	39.0	32.0	39.0
11	36.23540079262108	38.0	35.0	39.0	32.0	39.0
12	36.085361942983184	37.0	35.0	39.0	32.0	39.0
13	36.24787948798094	38.0	35.0	39.0	32.0	39.0
14	37.16114564412257	39.0	36.0	40.0	32.0	41.0
15	37.160436840956805	39.0	36.0	40.0	32.0	41.0
16	37.09426308060673	39.0	36.0	40.0	32.0	41.0
17	36.99116373463707	38.0	36.0	40.0	31.0	41.0
18	37.0015208122007	38.0	36.0	40.0	31.0	41.0
19	37.039923347526596	39.0	36.0	40.0	31.0	41.0
20	36.89664706246609	39.0	36.0	40.0	31.0	41.0
21	36.88863976138803	39.0	36.0	40.0	31.0	41.0
22	36.77419329869548	39.0	36.0	40.0	31.0	41.0
23	36.704188020452456	38.0	36.0	40.0	31.0	41.0
24	36.577299353046634	38.0	35.0	40.0	30.0	41.0
25	36.55176990693779	38.0	35.0	40.0	30.0	41.0
26	36.36081767061405	38.0	35.0	40.0	30.0	41.0
27	36.1361499197943	38.0	35.0	40.0	29.0	41.0
28	36.00508620638815	38.0	34.0	40.0	29.0	41.0
29	35.90684269656295	38.0	34.0	40.0	29.0	41.0
30	35.840469159137555	38.0	34.0	40.0	29.0	41.0
31	35.79817244521243	38.0	34.0	40.0	29.0	41.0
32	35.68299764395744	38.0	34.0	40.0	28.0	41.0
33	35.65858208405086	38.0	34.0	40.0	28.0	41.0
34	35.65227399389021	38.0	34.0	40.0	28.0	41.0
35	35.53762038227454	38.0	34.0	40.0	27.0	41.0
36	35.44422902266991	38.0	34.0	40.0	27.0	41.0
37	35.27756481328584	38.0	34.0	40.0	27.0	41.0
38	35.21972934997995	38.0	34.0	40.0	27.0	41.0
39	35.105045145195916	38.0	33.0	40.0	26.0	41.0
40	35.03145383159161	38.0	33.0	40.0	26.0	41.0
41	34.965714273076834	38.0	33.0	40.0	26.0	41.0
42	34.9079606372815	38.0	33.0	40.0	26.0	41.0
43	33.55702750878724	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	2.0
12	1.0
13	6.0
14	3.0
15	11.0
16	25.0
17	48.0
18	140.0
19	339.0
20	804.0
21	1586.0
22	3124.0
23	5331.0
24	8867.0
25	14558.0
26	21990.0
27	32070.0
28	45923.0
29	62633.0
30	82882.0
31	106308.0
32	135068.0
33	169376.0
34	214487.0
35	274795.0
36	356741.0
37	467121.0
38	534782.0
39	173998.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.24394034111014	19.58132327616711	13.420227760609563	25.754508622113185
2	18.66533432804133	21.275005307730417	35.403593923238425	24.656066440989832
3	20.191454259158785	22.885496036894622	30.091624696279872	26.83142500766672
4	15.573839376282702	16.204353518435514	33.80710970489019	34.41469740039159
5	15.456811292491333	35.67034423580477	33.82402809558633	15.048816376117571
6	35.193643697954755	34.34392766153193	15.126110200278362	15.336318440234956
7	28.861558172725342	30.376251739756082	21.46578872873959	19.296401358778986
8	25.94665583496497	35.30256274916845	19.724337123446016	19.02644429242056
9	26.313773855299473	15.036947701162983	19.872032094076573	38.77724634946097
10	17.185841334245477	26.778495140477933	33.200517208841504	22.835146316435093
11	35.421581231865254	21.927634993276875	21.522809971456205	21.127973803401666
12	21.086728314972518	24.889754016182682	30.5237255549527	23.4997921138921
13	30.613477801891907	20.018068398952607	24.546225908801397	24.822227890354085
14	22.056273737349912	20.806524380175038	25.888491955839683	31.24870992663537
15	24.951161508339034	27.675649017480126	22.866108077186194	24.507081396994646
16	24.19532595362223	26.30337954990446	25.00762986247081	24.493664634002503
17	23.76248791016961	26.45125881672997	25.784954353518437	24.001298919581988
18	23.891790120544453	25.553404614186974	26.55339576796962	24.001409497298955
19	24.701145290273878	25.435528767898845	26.819998643580007	23.043327298247267
20	24.224186737750937	25.214594489396337	26.806213288197966	23.755005484654763
21	23.865362046189052	25.551082482130642	27.066513233941166	23.51704223773914
22	24.38408211648699	25.187097497110233	26.3575257719799	24.07129461442287
23	23.84929141798967	25.439546424948695	26.456787702578378	24.254374454483262
24	23.835727218041566	25.393251220777994	26.486864841593732	24.284156719586704
25	24.3367179943856	25.170473980326015	26.370094772475287	24.122713252813096
26	23.93001315137647	25.404567007147744	26.643148014908824	24.02227182656696
27	24.18655345474275	25.818717416432733	26.165415418367104	23.82931371045741
28	23.957030973555707	25.732761671109433	26.18432420796867	24.125883147366185
29	23.86783161520134	25.92442971385436	26.589554681418225	23.618183989526077
30	24.2789595668892	25.66357687952631	26.271459448939634	23.786004104644853
31	24.04280242268406	25.263138107145384	26.705366410322945	23.98869305984761
32	23.640889280743554	25.695349543535183	26.509643851289187	24.15411732443207
33	23.706867318534595	25.254623622938833	26.500023589912953	24.538485468613622
34	24.071626347573776	25.2028732513977	26.86474575971315	23.860754641315374
35	23.87037490269161	25.244008162109882	26.78929489750183	24.09632203769668
36	23.748481399353636	24.962182420796868	26.97031062017881	24.319025559670685
37	23.526957373027294	24.675085808308367	27.44103996131254	24.356916857351795
38	23.411956547380342	24.52993412516808	27.70465723856479	24.353452088886794
39	23.050109398221323	24.53922265339341	28.148184461324337	24.262483487060933
40	23.34649453893515	24.38209171758156	28.14729983958859	24.124113903894695
41	22.84458228161638	24.526358778986104	28.21607917954283	24.412979759854686
42	22.52950950673492	24.829747175107926	28.16255956453021	24.47818375362695
43	22.478791193885495	24.503506050812675	28.038196492179946	24.97950626312189
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2041.0
1	1693.0
2	1345.0
3	2238.5
4	3132.0
5	3132.0
6	3747.0
7	4362.0
8	4084.0
9	3806.0
10	5306.5
11	6807.0
12	6807.0
13	10549.0
14	14291.0
15	17532.5
16	20774.0
17	19371.5
18	17969.0
19	17969.0
20	20865.5
21	23762.0
22	21705.0
23	19648.0
24	22762.5
25	25877.0
26	25877.0
27	31048.0
28	36219.0
29	41677.0
30	47135.0
31	54575.0
32	62015.0
33	62015.0
34	71189.5
35	80364.0
36	91414.5
37	102465.0
38	114741.5
39	127018.0
40	127018.0
41	140539.5
42	154061.0
43	168465.0
44	182869.0
45	196410.5
46	209952.0
47	209952.0
48	221893.0
49	233834.0
50	238514.0
51	243194.0
52	240344.0
53	237494.0
54	237494.0
55	225153.0
56	212812.0
57	197369.5
58	181927.0
59	165236.5
60	148546.0
61	148546.0
62	129111.5
63	109677.0
64	92407.0
65	75137.0
66	61872.5
67	48608.0
68	48608.0
69	39515.5
70	30423.0
71	24529.0
72	18635.0
73	14986.5
74	11338.0
75	11338.0
76	9137.5
77	6937.0
78	5554.0
79	4171.0
80	3238.5
81	2306.0
82	2306.0
83	1740.0
84	1174.0
85	865.0
86	556.0
87	405.5
88	255.0
89	255.0
90	158.5
91	62.0
92	39.5
93	17.0
94	12.0
95	7.0
96	7.0
97	4.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2713024.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.35531127405257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.71449949317321	53.87472673034678
2	8.570771481628112	11.031493331178998
3	2.8984841707981754	5.595985531038923
4	1.3832443459731598	3.560764818127039
5	0.8248217237593323	2.654082938906621
6	0.5449789807702908	2.1043375167172784
7	0.3755240165351524	1.6916875482501537
8	0.276617441504124	1.4241441241463941
9	0.21507593210079892	1.245715070211352
>10	1.1443871599042916	12.72921089033867
>50	0.0412868730523823	1.7678109995500366
>100	0.009621062109130494	0.9315420642607257
>500	1.1455311531494764E-4	0.04548586559786225
>1k	4.00935903592506E-4	0.4661379710999714
>5k	1.7182967296821685E-4	0.8768746002291017
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9273	0.3417957231487816	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8348	0.3077009270835791	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6168	0.22734778608666933	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4651	0.1714323205397372	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.685923898940813E-5	0.0	0.0	0.0	0.0
3	3.685923898940813E-5	0.0	0.0	0.0	0.0
4	3.685923898940813E-5	0.0	0.0	0.0	3.685923898940813E-5
5	3.685923898940813E-5	0.0	0.0	0.0	3.685923898940813E-5
6	3.685923898940813E-5	0.0	0.0	0.0	3.685923898940813E-5
7	3.685923898940813E-5	0.0	0.0	0.0	3.685923898940813E-5
8	3.685923898940813E-5	0.0	0.0	0.0	3.685923898940813E-5
9	7.371847797881626E-5	0.0	0.0	0.0	1.1057771696822438E-4
10	1.1057771696822438E-4	0.0	0.0	0.0	1.4743695595763253E-4
11	1.4743695595763253E-4	0.0	0.0	0.0	1.4743695595763253E-4
12	1.8429619494704063E-4	0.0	0.0	0.0	1.8429619494704063E-4
13	1.8429619494704063E-4	0.0	0.0	0.0	1.8429619494704063E-4
14	1.8429619494704063E-4	0.0	0.0	3.685923898940813E-5	1.8429619494704063E-4
15	1.8429619494704063E-4	0.0	0.0	3.685923898940813E-5	2.580146729258569E-4
16	1.8429619494704063E-4	0.0	0.0	3.685923898940813E-5	2.580146729258569E-4
17	1.8429619494704063E-4	0.0	0.0	1.4743695595763253E-4	2.580146729258569E-4
18	1.8429619494704063E-4	0.0	0.0	1.4743695595763253E-4	2.9487391191526506E-4
19	1.8429619494704063E-4	0.0	0.0	1.4743695595763253E-4	2.9487391191526506E-4
20	1.8429619494704063E-4	0.0	0.0	2.2115543393644876E-4	2.9487391191526506E-4
21	1.8429619494704063E-4	0.0	0.0	4.054516288834894E-4	2.9487391191526506E-4
22	2.2115543393644876E-4	0.0	0.0	7.003255407987544E-4	2.9487391191526506E-4
23	2.2115543393644876E-4	0.0	0.0	0.0010320586917034276	3.6859238989408127E-4
24	2.2115543393644876E-4	3.685923898940813E-5	0.0	0.0018061027104809982	3.6859238989408127E-4
25	2.2115543393644876E-4	3.685923898940813E-5	0.0	0.0026907244462267936	3.6859238989408127E-4
26	2.2115543393644876E-4	3.685923898940813E-5	0.0	0.0053077304144747706	3.6859238989408127E-4
27	2.2115543393644876E-4	3.685923898940813E-5	0.0	0.020825470029015594	3.6859238989408127E-4
28	2.2115543393644876E-4	3.685923898940813E-5	0.0	0.06465110518742186	3.6859238989408127E-4
29	2.2115543393644876E-4	3.685923898940813E-5	0.0	0.13449936307235028	4.054516288834894E-4
30	2.2115543393644876E-4	3.685923898940813E-5	0.0	0.218649005685169	4.054516288834894E-4
31	2.2115543393644876E-4	3.685923898940813E-5	0.0	0.4735675025359156	4.054516288834894E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3695	0.0	18.124493	1
GTATACG	100	5.883723E-6	16.65	1
TCTTATA	2700	0.0	15.074074	37
CTTATAC	1635	0.0	15.048929	37
CTCTTAT	4210	0.0	13.446555	37
GTATCAA	5070	0.0	13.063116	2
TACCCGT	145	1.3734916E-5	12.75862	6
TTAGTAC	165	3.80834E-6	12.333333	3
GTATAGG	325	0.0	11.953847	1
GTCTATA	190	1.4356665E-6	11.684211	1
TAGACTG	320	5.456968E-12	11.562501	5
TATTCCG	145	1.9400632E-4	11.4827585	5
GATATAA	230	1.0246367E-7	11.26087	1
TATAGGG	230	1.0246367E-7	11.26087	2
GTATTAC	200	2.6605321E-6	11.099999	1
TAGTACT	255	3.637615E-8	10.882353	4
GTAAGAG	1210	0.0	10.855371	3
AGGCCGT	290	1.895387E-9	10.844828	6
CTTACAC	465	0.0	10.741936	3
ACCCGAC	1035	0.0	10.724638	11
>>END_MODULE