##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727092.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8208025
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00976190983824	33.0	31.0	34.0	30.0	34.0
2	32.228202033985035	33.0	31.0	34.0	30.0	34.0
3	32.33344074853573	34.0	31.0	34.0	30.0	34.0
4	35.94886723663731	37.0	35.0	37.0	35.0	37.0
5	35.65738773943793	37.0	35.0	37.0	33.0	37.0
6	35.67551463354461	37.0	35.0	37.0	33.0	37.0
7	35.63570907739682	37.0	35.0	37.0	33.0	37.0
8	35.6047956725278	37.0	35.0	37.0	33.0	37.0
9	37.42754694338772	39.0	37.0	39.0	35.0	39.0
10	37.249073315444335	39.0	37.0	39.0	34.0	39.0
11	37.33887603899842	39.0	37.0	39.0	34.0	39.0
12	37.24616372391653	39.0	37.0	39.0	34.0	39.0
13	37.31960502069621	39.0	37.0	39.0	34.0	39.0
14	38.49803040317251	40.0	38.0	41.0	34.0	41.0
15	38.451540535025174	40.0	38.0	41.0	34.0	41.0
16	38.405828929614614	40.0	38.0	41.0	34.0	41.0
17	38.371045897155525	40.0	38.0	41.0	34.0	41.0
18	38.37513092369967	40.0	38.0	41.0	34.0	41.0
19	38.42268475059469	40.0	38.0	41.0	34.0	41.0
20	38.34181450470729	40.0	38.0	41.0	34.0	41.0
21	38.28057431598953	40.0	38.0	41.0	34.0	41.0
22	38.21570292488144	40.0	38.0	41.0	34.0	41.0
23	38.15281325288361	40.0	38.0	41.0	33.0	41.0
24	38.07325867063027	40.0	37.0	41.0	33.0	41.0
25	38.039176781259805	40.0	37.0	41.0	33.0	41.0
26	37.87202305061205	40.0	37.0	41.0	33.0	41.0
27	37.735319519616475	40.0	37.0	41.0	33.0	41.0
28	37.648852312218835	40.0	37.0	41.0	32.0	41.0
29	37.550657923191025	39.0	37.0	41.0	32.0	41.0
30	37.48516189948252	39.0	37.0	41.0	32.0	41.0
31	37.424948632588226	39.0	36.0	41.0	32.0	41.0
32	37.31091340486901	39.0	36.0	41.0	32.0	41.0
33	37.22526892888362	39.0	36.0	41.0	31.0	41.0
34	37.17377907596529	39.0	36.0	41.0	31.0	41.0
35	37.09003993043393	39.0	36.0	41.0	31.0	41.0
36	36.95933711215548	39.0	36.0	40.0	31.0	41.0
37	36.86707326061994	39.0	35.0	40.0	31.0	41.0
38	36.811815753485156	39.0	35.0	40.0	30.0	41.0
39	36.69419598502685	39.0	35.0	40.0	30.0	41.0
40	36.591404509611024	39.0	35.0	40.0	30.0	41.0
41	36.520774973273106	39.0	35.0	40.0	30.0	41.0
42	36.36537120683721	39.0	35.0	40.0	30.0	41.0
43	35.06363906055354	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	14.0
10	20.0
11	18.0
12	13.0
13	9.0
14	6.0
15	27.0
16	45.0
17	82.0
18	212.0
19	477.0
20	1068.0
21	2264.0
22	4222.0
23	7615.0
24	13082.0
25	21034.0
26	32532.0
27	49116.0
28	70815.0
29	99014.0
30	133923.0
31	177599.0
32	233570.0
33	307102.0
34	411159.0
35	562888.0
36	805575.0
37	1235876.0
38	2113077.0
39	1925570.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.20390106024287	18.17018101187557	12.765177981304882	25.860739946576672
2	18.563783614206827	20.669235291071846	35.63128767273491	25.13569342198641
3	20.282601478431168	22.27069240164351	29.793561788615406	27.65314433130991
4	15.20059941337898	15.426280987204596	33.518160580651255	35.85495901876517
5	15.788901715089807	35.24282638028027	33.45525872545466	15.513013179175259
6	35.61973800031067	33.5558919472102	14.97256648219273	15.851803570286396
7	29.36779554155841	29.96295698417098	21.16259392484794	19.50665354942267
8	26.591792788155495	34.573457074022066	19.45400994758179	19.380740190240648
9	26.61150520374877	14.881996582612746	19.64415313062521	38.862345083013274
10	17.73642258643218	26.34693485948203	32.41488665056454	23.50175590352125
11	35.44414155658639	21.786422434142196	21.132282126333678	21.637153882937735
12	21.607975121908133	24.920282284715263	29.96929224752605	23.502450345850555
13	31.17166431632457	19.55941898325115	24.226534397738796	25.042382302685485
14	22.35096749827151	20.65746632106993	25.79363488780797	31.197931292850594
15	25.384888569418347	27.18457850700991	22.770142634799477	24.66039028877227
16	24.344309380149305	25.86775746906229	24.905979209371317	24.88195394141709
17	24.036988191434602	26.085081368538717	25.648155311417792	24.229775128608892
18	24.137450848407504	25.064044030080318	26.420618845581	24.377886275931175
19	25.116188120772048	24.951654021521623	26.74419242144121	23.187965436265117
20	24.548536340959	24.967077951151463	26.582058412346456	23.90232729554308
21	24.12789922057986	25.330344388570943	26.818217537105454	23.723538853743744
22	24.59825597509754	25.04583014793449	26.143719104169392	24.212194772798572
23	24.03960757916795	25.1888487181752	26.346776477898153	24.424767224758696
24	24.003703692422963	25.25158000858915	26.420009685643013	24.32470661334487
25	24.40657770901039	24.944648682234764	26.227527328437716	24.42124628031713
26	23.94049725725738	25.39285638140722	26.543437672278046	24.123208689057353
27	24.302801221974835	25.600397172279564	26.250577940491166	23.84622366525443
28	24.11316973327932	25.60980260172209	26.152539740071457	24.12448792492713
29	23.950938258594483	25.82004806271911	26.496836937022	23.732176741664407
30	24.146368949899642	25.64532880930553	26.326028490410298	23.882273750384535
31	24.064193274265126	25.120549705928042	26.582935602657155	24.23232141714968
32	23.531763122066515	25.627005278370863	26.53205856463644	24.309173034926186
33	23.743726414088652	25.15752571416388	26.467792191178756	24.63095568056871
34	23.997478077856734	25.13334206462578	26.79693348887217	24.07224636864532
35	23.745395512318737	25.168514959445176	26.793704941200836	24.292384587035247
36	23.5597357464189	24.840238668863705	27.1088477435193	24.491177841198095
37	23.305192174731438	24.475632079580652	27.44561572363632	24.77356002205159
38	23.235309346645508	24.397537775530655	27.84365301031612	24.523499867507713
39	22.85832462742255	24.30790598225517	28.346636371112417	24.487133019209857
40	23.090170899820603	24.253166870227613	28.39055680264132	24.266105427310467
41	22.564880102095206	24.361353675214197	28.547512952263183	24.526253270427418
42	22.18021996765361	24.689995461758464	28.444686754730885	24.685097815857045
43	22.065710082510716	24.366738649066004	28.34310324347209	25.22444802495119
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5478.0
1	4583.5
2	3689.0
3	7168.5
4	10648.0
5	10648.0
6	13028.5
7	15409.0
8	14473.5
9	13538.0
10	19618.0
11	25698.0
12	25698.0
13	41006.5
14	56315.0
15	71623.0
16	86931.0
17	78314.5
18	69698.0
19	69698.0
20	75480.0
21	81262.0
22	67983.0
23	54704.0
24	63027.5
25	71351.0
26	71351.0
27	84712.0
28	98073.0
29	112252.0
30	126431.0
31	146142.0
32	165853.0
33	165853.0
34	190364.5
35	214876.0
36	246799.0
37	278722.0
38	317319.5
39	355917.0
40	355917.0
41	396026.5
42	436136.0
43	481265.0
44	526394.0
45	569747.0
46	613100.0
47	613100.0
48	652479.5
49	691859.0
50	711473.0
51	731087.0
52	726739.0
53	722391.0
54	722391.0
55	687980.0
56	653569.0
57	609982.5
58	566396.0
59	518350.5
60	470305.0
61	470305.0
62	412885.5
63	355466.0
64	302586.5
65	249707.0
66	208433.0
67	167159.0
68	167159.0
69	137965.5
70	108772.0
71	89842.0
72	70912.0
73	57864.5
74	44817.0
75	44817.0
76	36494.0
77	28171.0
78	22712.0
79	17253.0
80	13496.0
81	9739.0
82	9739.0
83	7610.0
84	5481.0
85	4145.5
86	2810.0
87	2054.0
88	1298.0
89	1298.0
90	861.0
91	424.0
92	272.5
93	121.0
94	86.5
95	52.0
96	52.0
97	30.0
98	8.0
99	6.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8208025.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.27572060924105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.18199291563185	40.601137460248424
2	10.469413383829647	10.736534312237959
3	3.702612493568841	5.695623712335635
4	1.7606513898086884	3.6111467501640897
5	1.038176111771697	2.661661412519687
6	0.6908530186697686	2.1254391820417102
7	0.5007537470686096	1.7973556460104407
8	0.3740145280680681	1.5342291556012324
9	0.2791556244300926	1.288251522429809
>10	1.8040950041451576	17.415973067966878
>50	0.1398808528262047	4.8857464928688366
>100	0.05554485752028083	4.793375239832498
>500	0.002261222709533705	0.7533489016168186
>1k	4.044979770992357E-4	0.38025435076318675
>5k	9.517598724794802E-5	0.28243037747871796
>10k+	9.517598724794802E-5	1.4374924158839477
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37213	0.45337337544659034	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34707	0.422842279354656	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26998	0.3289220001157404	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18904	0.2303111893543209	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.65495962792511E-5
2	0.0	0.0	0.0	1.2183198759750366E-5	3.65495962792511E-5
3	0.0	0.0	0.0	1.2183198759750366E-5	3.65495962792511E-5
4	0.0	0.0	0.0	1.2183198759750366E-5	3.65495962792511E-5
5	2.4366397519500732E-5	1.2183198759750366E-5	0.0	1.2183198759750366E-5	3.65495962792511E-5
6	2.4366397519500732E-5	1.2183198759750366E-5	0.0	1.2183198759750366E-5	1.096487888377533E-4
7	2.4366397519500732E-5	1.2183198759750366E-5	0.0	1.2183198759750366E-5	1.096487888377533E-4
8	6.091599379875183E-5	1.2183198759750366E-5	0.0	1.2183198759750366E-5	1.3401518635725403E-4
9	6.091599379875183E-5	1.2183198759750366E-5	0.0	3.65495962792511E-5	1.5838158387675475E-4
10	8.528239131825257E-5	1.2183198759750366E-5	0.0	8.528239131825257E-5	1.7056478263650514E-4
11	1.096487888377533E-4	1.2183198759750366E-5	0.0	1.096487888377533E-4	1.9493118015600586E-4
12	1.096487888377533E-4	2.4366397519500732E-5	0.0	1.461983851170044E-4	1.9493118015600586E-4
13	1.3401518635725403E-4	2.4366397519500732E-5	0.0	1.7056478263650514E-4	2.0711437891575622E-4
14	1.827479813962555E-4	2.4366397519500732E-5	0.0	2.4366397519500732E-4	3.0457996899375917E-4
15	2.0711437891575622E-4	3.65495962792511E-5	0.0	3.289463665132599E-4	3.65495962792511E-4
16	2.4366397519500732E-4	3.65495962792511E-5	0.0	5.482439441887665E-4	4.1422875783151243E-4
17	2.4366397519500732E-4	3.65495962792511E-5	0.0	8.284575156630249E-4	4.1422875783151243E-4
18	2.802135714742584E-4	3.65495962792511E-5	0.0	9.62472702020279E-4	4.385951553510132E-4
19	2.802135714742584E-4	3.65495962792511E-5	0.0	0.0012183198759750367	4.385951553510132E-4
20	2.923967702340088E-4	4.8732795039001464E-5	0.0	0.0017543806214040528	4.385951553510132E-4
21	3.167631677535095E-4	4.8732795039001464E-5	0.0	0.0030457996899375915	4.385951553510132E-4
22	3.289463665132599E-4	4.8732795039001464E-5	0.0	0.004617432329945389	4.385951553510132E-4
23	3.289463665132599E-4	4.8732795039001464E-5	0.0	0.005787019410881424	6.091599379875183E-4
24	3.289463665132599E-4	4.8732795039001464E-5	0.0	0.008053094380194992	6.213431367472687E-4
25	3.289463665132599E-4	4.8732795039001464E-5	0.0	0.009880574194157547	6.213431367472687E-4
26	3.289463665132599E-4	4.8732795039001464E-5	0.0	0.014120327362550674	6.33526335507019E-4
27	3.289463665132599E-4	4.8732795039001464E-5	0.0	0.03908370162127917	6.457095342667695E-4
28	3.289463665132599E-4	4.8732795039001464E-5	0.0	0.1100873839931043	6.457095342667695E-4
29	3.289463665132599E-4	4.8732795039001464E-5	0.0	0.2246703683285565	6.457095342667695E-4
30	3.289463665132599E-4	6.091599379875183E-5	0.0	0.36034246971713657	6.457095342667695E-4
31	3.289463665132599E-4	6.091599379875183E-5	0.0	0.7216230457387739	6.457095342667695E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	6865	0.0	15.576111	37
GGTATCA	21895	0.0	13.476821	1
TCTTATA	10615	0.0	13.454545	37
TATACCG	340	0.0	13.058824	5
ATACCCG	720	0.0	12.847222	5
TATACAC	2995	0.0	12.106844	37
CTCTTAT	16130	0.0	12.100123	37
TACTTAC	1720	0.0	11.831395	31
TACCCGT	790	0.0	11.474684	6
TATACTG	1070	0.0	11.411215	5
ACCCGTT	910	0.0	10.978023	7
GGAGTGT	2720	0.0	10.950368	6
AGTGTAC	2205	0.0	10.739229	27
ATTATAC	1120	0.0	10.736607	3
TCGTTAC	935	0.0	10.684491	23
TCTCTTA	22595	0.0	10.578446	37
GTATCAA	28395	0.0	10.424371	2
GTACTTA	2035	0.0	10.363635	30
TACACCG	590	0.0	10.347458	5
GAGTGTC	2350	0.0	10.155319	7
>>END_MODULE