FastQCFastQC Report
Thu 9 Feb 2017
SRR2727091.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727091.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13653268
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT291730.21367045604026813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT278280.2038193346823632No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT227430.16657550412106464No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164340.12036678691138268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122550.019.337821
CTTATAC73050.016.05612837
TCTTATA120050.014.79383637
GTATCAA175500.013.4507132
CTCTTAT183400.012.04416737
TCGTTAC14700.011.4523823
TATACTG19500.011.3846155
TAGATCG3750.011.3466675
TCTATAC14600.011.1506853
CTACACT27200.010.6102944
TACTTAC22950.010.55991431
TACGGAT29900.010.51839527
TAATACT23450.010.4925374
AACGCAG226300.010.4885117
GTCTTAG22950.010.3180831
GGGTAAG49600.010.2570571
TTATACT26400.010.2310614
GTTACGG22250.010.22696625
TATCAAC237750.010.2246063
ACGGATG31100.010.17202528
Produced by FastQC (version 0.11.5)