FastQCFastQC Report
Thu 9 Feb 2017
SRR2727090.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727090.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8991544
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT305810.34010843966286547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT298570.3320564299079224No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT225400.25067997220499616No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167640.18644183913241152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC75650.015.94448137
GGTATCA155750.015.7858741
TCTTATA118200.013.36632837
CGTATAC2703.6379788E-1213.01851753
TATACTG13150.012.5209125
CTCTTAT179100.012.353991537
TATACAC32350.011.83771237
GTATCAA209300.011.7646912
TTATACT15600.011.1474364
GTACCGT4500.011.16
TCTATAC9800.010.7602033
TAGATCG2952.5174813E-910.6610165
CTATACA13600.010.6102944
TAATACT12450.010.55024
TATACCG4250.010.4470595
AACGCAG230650.010.4029917
TCTCTTA249700.010.38726537
TAGACAG16100.010.3416155
GGAGTGT28300.010.3286226
TATACTC8500.010.2294125
Produced by FastQC (version 0.11.5)