FastQCFastQC Report
Thu 9 Feb 2017
SRR2727089.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727089.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666562
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT129950.4873316277663898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT119220.447092548382524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT75840.2844111631381532No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62480.23430919663596797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59050.016.3852671
CTTATAC14100.016.13829837
TAGACGG800.00630149613.8750017
ATTACGA850.0094089913.05882334
GGGTAAG9550.012.9790571
GTATTAG2951.8189894E-1212.5423731
GTATCAA77900.012.3729152
TAAACGT1050.002645147312.3333334
CTCTTAT31950.012.10172137
TCTTATA22100.011.97058837
GATGTGT9500.011.2947376
TAGTACG1150.0052215311.260872
GTAAGAG9750.011.1948723
GTGTATA6800.011.1544119
CTTAGAC4050.010.9629633
CGACGCG1200.007166550510.79166617
TACGCGA1200.007166550510.79166618
TACACAG7900.010.7721525
TTAGACA5000.010.734
TCGTTAC2952.5120244E-910.66101623
Produced by FastQC (version 0.11.5)