##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727089.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2666562
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.78270634622409	31.0	30.0	31.0	26.0	34.0
2	30.01328752153522	31.0	30.0	31.0	26.0	34.0
3	30.06418976944845	31.0	30.0	31.0	26.0	34.0
4	33.72636188470398	35.0	33.0	37.0	28.0	37.0
5	31.995475447411312	35.0	30.0	35.0	22.0	37.0
6	32.208620313347296	35.0	32.0	35.0	25.0	37.0
7	32.22485507556171	35.0	32.0	35.0	25.0	37.0
8	32.11892616785209	35.0	32.0	35.0	25.0	37.0
9	33.450412178678015	35.0	32.0	38.0	26.0	39.0
10	32.88681118233891	35.0	31.0	37.0	25.0	39.0
11	33.29502220462153	35.0	32.0	37.0	25.0	39.0
12	32.98385561633294	35.0	31.0	37.0	25.0	39.0
13	33.266720593783305	35.0	32.0	37.0	25.0	39.0
14	33.77361523939815	36.0	32.0	38.0	25.0	40.0
15	33.737860586028	36.0	32.0	38.0	25.0	40.0
16	33.61488575926605	36.0	31.0	38.0	25.0	40.0
17	33.53484674273465	36.0	31.0	38.0	25.0	40.0
18	33.499187343103216	36.0	31.0	38.0	24.0	40.0
19	33.540205703073845	36.0	31.0	39.0	24.0	40.0
20	33.38581139309718	36.0	31.0	39.0	24.0	40.0
21	33.256411439148984	36.0	31.0	39.0	24.0	40.0
22	33.160940566917255	36.0	31.0	39.0	23.0	40.0
23	33.01406942722502	36.0	30.0	38.0	23.0	40.0
24	32.83128650299524	36.0	30.0	38.0	21.0	40.0
25	32.77715200321612	35.0	30.0	38.0	21.0	40.0
26	32.39682595041855	35.0	30.0	38.0	19.0	40.0
27	32.14923260737984	35.0	30.0	38.0	18.0	40.0
28	31.966041667135435	35.0	29.0	38.0	18.0	40.0
29	31.765994565286686	35.0	29.0	38.0	17.0	40.0
30	31.6331448509354	34.0	29.0	38.0	17.0	40.0
31	31.523486796856776	34.0	28.0	38.0	17.0	40.0
32	31.328124003867153	34.0	27.0	38.0	16.0	40.0
33	31.169779288837088	34.0	27.0	38.0	16.0	40.0
34	31.060662756013173	34.0	27.0	38.0	16.0	40.0
35	30.897500226883906	34.0	27.0	38.0	15.0	40.0
36	30.64683288819086	34.0	26.0	38.0	15.0	40.0
37	30.52821685751166	34.0	26.0	38.0	15.0	40.0
38	30.414010999931747	33.0	26.0	38.0	15.0	40.0
39	30.230094031190724	33.0	25.0	38.0	14.0	40.0
40	30.08479645326079	33.0	25.0	38.0	13.0	40.0
41	29.94343052964829	33.0	25.0	38.0	13.0	40.0
42	29.67697844640402	33.0	25.0	37.0	12.0	39.0
43	28.415589811900116	31.0	23.0	37.0	9.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	22.0
11	38.0
12	78.0
13	183.0
14	399.0
15	808.0
16	1580.0
17	3082.0
18	5490.0
19	9252.0
20	15045.0
21	22627.0
22	32754.0
23	46105.0
24	61949.0
25	80506.0
26	101219.0
27	124040.0
28	147367.0
29	170755.0
30	192275.0
31	211018.0
32	226189.0
33	234056.0
34	232689.0
35	219435.0
36	192748.0
37	160049.0
38	125829.0
39	48970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.96175599892296	21.707127004734936	14.175181375869004	24.155935620473105
2	20.647297906442827	23.44569524353831	32.895616152934004	23.01139069708486
3	22.247635719701996	24.035180880849573	29.32776361472188	24.389419784726552
4	18.151387441957098	17.262340046846838	32.505300833057696	32.08097167813836
5	15.57424878926498	36.76786813882445	32.64101865998241	15.016864411928168
6	34.80811621856158	35.18958119106175	14.466605314258585	15.535697276118087
7	28.789654993958514	30.90597555954071	21.10939104359846	19.19497840290231
8	26.569230342290933	35.56410839125436	19.082136473856597	18.78452479259811
9	26.761312881530603	14.946474149110353	19.50571559933727	38.78649737002177
10	17.504037033453564	27.678298873230773	32.35927010135148	22.458393991964183
11	35.86948287720293	22.107942736752417	20.981548525779637	21.041025860265012
12	21.325887041066363	25.737072680102695	29.991802178235496	22.945238100595446
13	31.27660260665231	20.085300848058285	24.433559017191424	24.204537528097976
14	22.119268181276112	21.211132536952075	26.11463749952186	30.55496178224995
15	25.319006270996137	27.954347208127917	22.76511853090234	23.961527989973607
16	24.130134607783354	26.47423911388522	25.10986056202706	24.285765716304365
17	23.956352786846885	26.650083515777993	25.79354989683345	23.60001380054167
18	24.09904588755109	25.464324474735633	26.58434343547984	23.85228620223344
19	25.14728703101597	25.28446741534605	27.03203600741329	22.53620954622469
20	24.542650799043862	25.298567968792774	26.840328482892957	23.31845274927041
21	24.15357302774134	25.520989198826054	27.137715155319846	23.187722618112762
22	24.4797608306126	25.41167240814202	26.593268785799847	23.515297975445538
23	24.057756766953105	25.502425970219335	26.77335085402102	23.666466408806546
24	23.988829061540663	25.614330362466724	26.63680799471379	23.760032581278814
25	24.447659570638148	25.28202981967042	26.66137145882976	23.608939150861673
26	24.06341948921495	25.592504505801855	26.803989556590096	23.5400864483931
27	24.40213278371176	25.871927973172948	26.436362627233116	23.289576615882172
28	24.24515162220117	25.905416787608914	26.432949993287234	23.416481596902678
29	24.055956696300328	26.13121315011614	26.66587163546169	23.146958518121835
30	24.303578915472432	25.904629256698325	26.42136203846001	23.370429789369233
31	24.207350138492938	25.441486078328573	26.86980463983211	23.481359143346374
32	23.780620889369906	25.863602646403873	26.681284740426065	23.67449172380016
33	23.993854258779656	25.539664931848577	26.64314574347043	23.823335065901336
34	24.252539412171927	25.516976541329246	26.853491499541356	23.376992546957467
35	24.086857909172934	25.61935555970572	26.71949874032556	23.57428779079579
36	23.928339187313103	25.382383758562522	26.8194401630264	23.869836891097975
37	23.755569906118815	25.162812640396137	27.20698037397968	23.87463707950537
38	23.80747194327377	25.064183769212946	27.284608420880517	23.843735866632766
39	23.397730860936292	25.167425321443865	27.633072098079847	23.801771719539992
40	23.773458108230745	25.027319822303024	27.630934514179685	23.568287555286545
41	23.356029224147047	25.0382702521074	27.605583519153125	24.00011700459243
42	23.210185999800494	25.261103998331937	27.472265786432114	24.056444215435455
43	23.370729801144698	24.89336456455916	27.204317769472453	24.531587864823695
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1938.0
1	1629.0
2	1320.0
3	1929.5
4	2539.0
5	2539.0
6	3461.0
7	4383.0
8	4497.5
9	4612.0
10	6277.5
11	7943.0
12	7943.0
13	13621.5
14	19300.0
15	25476.0
16	31652.0
17	28343.0
18	25034.0
19	25034.0
20	27848.0
21	30662.0
22	26246.5
23	21831.0
24	24342.0
25	26853.0
26	26853.0
27	31273.0
28	35693.0
29	40541.5
30	45390.0
31	52113.5
32	58837.0
33	58837.0
34	67605.5
35	76374.0
36	86822.5
37	97271.0
38	109493.0
39	121715.0
40	121715.0
41	134690.0
42	147665.0
43	161287.0
44	174909.0
45	188077.5
46	201246.0
47	201246.0
48	211762.5
49	222279.0
50	226384.0
51	230489.0
52	227283.0
53	224077.0
54	224077.0
55	213549.0
56	203021.0
57	189564.0
58	176107.0
59	161321.5
60	146536.0
61	146536.0
62	128211.0
63	109886.0
64	93631.5
65	77377.0
66	64653.5
67	51930.0
68	51930.0
69	42700.0
70	33470.0
71	27540.0
72	21610.0
73	17404.0
74	13198.0
75	13198.0
76	10822.0
77	8446.0
78	6750.5
79	5055.0
80	3954.5
81	2854.0
82	2854.0
83	2194.5
84	1535.0
85	1184.5
86	834.0
87	651.5
88	469.0
89	469.0
90	314.5
91	160.0
92	99.5
93	39.0
94	29.0
95	19.0
96	19.0
97	11.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2666562.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.75745377448615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.84102862010204	62.15407522090185
2	6.542359149743018	9.258413502280563
3	2.148042423416784	4.55970037441645
4	1.0269371968471468	2.9065384494084947
5	0.5988182697259802	2.118542801972691
6	0.3842590251231251	1.631351412454718
7	0.27951076057565044	1.3844228804632133
8	0.19885850499878238	1.1256577180091816
9	0.15427641397116026	0.9824585607052075
>10	0.7880071148533655	9.6151286038624
>50	0.027789723724330154	1.3197147256145159
>100	0.009367210091851355	1.0426381186570053
>500	2.1302480766784954E-4	0.09616633321978524
>1k	3.1953721147553034E-4	0.3450230983153747
>5k	1.0651240382517677E-4	0.5212275552531961
>10k+	1.0651240382517677E-4	0.9389406444652897
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12995	0.4873316277663898	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11922	0.447092548382524	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7584	0.2844111631381532	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6248	0.23430919663596797	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.750147193277336E-5	0.0	0.0	0.0	0.0
2	3.750147193277336E-5	0.0	0.0	0.0	0.0
3	3.750147193277336E-5	0.0	0.0	0.0	0.0
4	3.750147193277336E-5	0.0	0.0	0.0	0.0
5	3.750147193277336E-5	0.0	0.0	0.0	3.750147193277336E-5
6	3.750147193277336E-5	0.0	0.0	0.0	3.750147193277336E-5
7	3.750147193277336E-5	0.0	0.0	3.750147193277336E-5	7.500294386554673E-5
8	3.750147193277336E-5	0.0	0.0	3.750147193277336E-5	7.500294386554673E-5
9	3.750147193277336E-5	0.0	0.0	1.1250441579832008E-4	7.500294386554673E-5
10	3.750147193277336E-5	0.0	0.0	1.5000588773109345E-4	7.500294386554673E-5
11	3.750147193277336E-5	0.0	0.0	1.5000588773109345E-4	7.500294386554673E-5
12	1.1250441579832008E-4	0.0	0.0	1.5000588773109345E-4	7.500294386554673E-5
13	1.1250441579832008E-4	0.0	0.0	1.875073596638668E-4	7.500294386554673E-5
14	1.1250441579832008E-4	0.0	0.0	2.6251030352941355E-4	1.1250441579832008E-4
15	1.1250441579832008E-4	3.750147193277336E-5	0.0	2.6251030352941355E-4	1.5000588773109345E-4
16	1.5000588773109345E-4	3.750147193277336E-5	0.0	3.000117754621869E-4	1.5000588773109345E-4
17	1.5000588773109345E-4	3.750147193277336E-5	0.0	3.750147193277336E-4	1.5000588773109345E-4
18	1.5000588773109345E-4	3.750147193277336E-5	0.0	4.5001766319328033E-4	1.5000588773109345E-4
19	1.5000588773109345E-4	3.750147193277336E-5	0.0	6.750264947899205E-4	1.5000588773109345E-4
20	1.875073596638668E-4	3.750147193277336E-5	0.0	9.37536798319334E-4	1.5000588773109345E-4
21	1.875073596638668E-4	3.750147193277336E-5	0.0	0.0017250677089075746	1.5000588773109345E-4
22	1.875073596638668E-4	3.750147193277336E-5	0.0	0.0026251030352941354	1.5000588773109345E-4
23	1.875073596638668E-4	3.750147193277336E-5	0.0	0.003112622170420189	2.2500883159664017E-4
24	1.875073596638668E-4	3.750147193277336E-5	0.0	0.0043126692722689364	2.2500883159664017E-4
25	1.875073596638668E-4	3.750147193277336E-5	0.0	0.005025197238991631	2.6251030352941355E-4
26	1.875073596638668E-4	3.750147193277336E-5	0.0	0.006825267891764752	2.6251030352941355E-4
27	1.875073596638668E-4	3.750147193277336E-5	0.0	0.01627563881882364	2.6251030352941355E-4
28	1.875073596638668E-4	3.750147193277336E-5	0.0	0.04728935610722721	2.6251030352941355E-4
29	1.875073596638668E-4	3.750147193277336E-5	0.0	0.10219151101680742	2.6251030352941355E-4
30	1.875073596638668E-4	3.750147193277336E-5	0.0	0.17175674145210199	2.6251030352941355E-4
31	1.875073596638668E-4	3.750147193277336E-5	0.0	0.3798149077351286	2.6251030352941355E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5905	0.0	16.385267	1
CTTATAC	1410	0.0	16.138298	37
TAGACGG	80	0.006301496	13.875001	7
ATTACGA	85	0.00940899	13.058823	34
GGGTAAG	955	0.0	12.979057	1
GTATTAG	295	1.8189894E-12	12.542373	1
GTATCAA	7790	0.0	12.372915	2
TAAACGT	105	0.0026451473	12.333333	4
CTCTTAT	3195	0.0	12.101721	37
TCTTATA	2210	0.0	11.970588	37
GATGTGT	950	0.0	11.294737	6
TAGTACG	115	0.00522153	11.26087	2
GTAAGAG	975	0.0	11.194872	3
GTGTATA	680	0.0	11.154411	9
CTTAGAC	405	0.0	10.962963	3
CGACGCG	120	0.0071665505	10.791666	17
TACGCGA	120	0.0071665505	10.791666	18
TACACAG	790	0.0	10.772152	5
TTAGACA	500	0.0	10.73	4
TCGTTAC	295	2.5120244E-9	10.661016	23
>>END_MODULE