FastQCFastQC Report
Thu 9 Feb 2017
SRR2727088.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727088.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1681058
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT67340.40058106264031346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60940.36250980037571573No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45210.26893777609100933No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31740.188809666293489No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG555.144453E-420.181825
CTTATAC12400.016.56048437
TATACAC4950.014.94949537
GGTATCA36700.014.7697531
ATACCGA800.006300219313.8756
TCTTATA20250.013.70370437
TCGTTAA850.00940709513.05882430
ACTGCGT1703.7336758E-713.0588248
TAGACAG2850.012.9824565
GAGTCGA1000.001833093112.9500016
TACACAG6200.012.8306465
GTATAAT2052.0667358E-812.6341471
TATACTG3150.012.3333345
CCTATAC1807.4671516E-712.3333343
TATACAG3150.012.3333345
TCTATAC1807.4671516E-712.3333343
CTCGTTA1653.8059043E-612.33333322
GTCTTAC1359.965359E-512.3333331
GTATCAA46000.011.7032612
TATACTA1901.4345915E-611.6842115
Produced by FastQC (version 0.11.5)