##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727087.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	814712
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63616345407948	31.0	31.0	34.0	30.0	34.0
2	31.87191424699771	31.0	31.0	34.0	30.0	34.0
3	31.959255540608215	33.0	31.0	34.0	30.0	34.0
4	35.70059235656281	37.0	35.0	37.0	33.0	37.0
5	35.320504669134614	37.0	35.0	37.0	33.0	37.0
6	35.35416441638272	37.0	35.0	37.0	33.0	37.0
7	35.29492016810848	37.0	35.0	37.0	32.0	37.0
8	35.26368704523807	37.0	35.0	37.0	32.0	37.0
9	37.09558224255933	39.0	37.0	39.0	33.0	39.0
10	36.87108941564627	39.0	37.0	39.0	33.0	39.0
11	36.98327507143629	39.0	37.0	39.0	33.0	39.0
12	36.867477096200865	39.0	37.0	39.0	33.0	39.0
13	37.017951128742425	39.0	37.0	39.0	33.0	39.0
14	38.00655446341775	40.0	37.0	41.0	33.0	41.0
15	38.015864501811684	40.0	37.0	41.0	33.0	41.0
16	37.93367226700969	39.0	37.0	41.0	33.0	41.0
17	37.90645037755673	39.0	37.0	41.0	33.0	41.0
18	37.94527145788941	39.0	37.0	41.0	33.0	41.0
19	37.982496882333876	40.0	37.0	41.0	33.0	41.0
20	37.865783246104144	39.0	37.0	41.0	33.0	41.0
21	37.857257533950644	39.0	37.0	41.0	33.0	41.0
22	37.76627200777698	39.0	37.0	41.0	33.0	41.0
23	37.696734551596144	39.0	37.0	41.0	32.0	41.0
24	37.57101282416363	39.0	37.0	41.0	32.0	41.0
25	37.54513496793959	39.0	36.0	41.0	32.0	41.0
26	37.38469299580711	39.0	36.0	40.0	32.0	41.0
27	37.160207533459676	39.0	36.0	40.0	31.0	41.0
28	37.02216488771492	39.0	36.0	40.0	31.0	41.0
29	36.874220583469985	39.0	35.0	40.0	31.0	41.0
30	36.81273750724182	39.0	35.0	40.0	31.0	41.0
31	36.68176361708186	39.0	35.0	40.0	30.0	41.0
32	36.50873805712939	38.0	35.0	40.0	30.0	41.0
33	36.41991648582567	38.0	35.0	40.0	30.0	41.0
34	36.37423654002887	38.0	35.0	40.0	30.0	41.0
35	36.21859871954752	38.0	35.0	40.0	30.0	41.0
36	36.094314309842005	38.0	35.0	40.0	30.0	41.0
37	35.902925205471384	38.0	35.0	40.0	29.0	41.0
38	35.7895833128762	38.0	35.0	40.0	28.0	41.0
39	35.621528834729325	38.0	35.0	40.0	27.0	41.0
40	35.482165476880176	38.0	34.0	40.0	27.0	41.0
41	35.34876987205295	38.0	34.0	40.0	26.0	41.0
42	35.198618652971845	38.0	34.0	40.0	25.0	41.0
43	33.936517444201144	37.0	33.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	6.0
12	5.0
13	3.0
14	2.0
15	5.0
16	2.0
17	10.0
18	20.0
19	55.0
20	182.0
21	295.0
22	580.0
23	1022.0
24	1759.0
25	2979.0
26	4581.0
27	6559.0
28	9613.0
29	13557.0
30	17948.0
31	23374.0
32	30301.0
33	39384.0
34	52103.0
35	69853.0
36	96620.0
37	135069.0
38	186012.0
39	122810.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.9449768752639	18.725881047535818	13.410874026649907	23.918268050550378
2	18.52004143795599	22.11628158171231	35.18998124490618	24.173695735425525
3	20.83803847249089	23.553353823191507	30.686303871797644	24.922303832519958
4	15.123626508508528	16.343321320908494	33.40346036390774	35.12959180667524
5	15.42422352929624	35.90557153939061	33.42800891603413	15.242196015279019
6	34.313720676754485	33.799796737988395	14.930552146034426	16.955930439222694
7	27.685979830909574	31.095896464026552	21.901359007845716	19.316764697218158
8	26.703424032050588	34.433763096652555	19.73138974263298	19.131423128663872
9	25.995812017007236	15.036601891220455	20.66963540490382	38.29795068686849
10	18.280570311963	27.057291410952583	32.060409077072634	22.601729200011782
11	33.49834051787626	22.87016761751392	21.308511473993264	22.32298039061656
12	21.17926334704779	26.619713469299583	30.144149098086192	22.056874085566434
13	31.15996818507644	19.97221103899292	25.239348383232358	23.62847239269828
14	21.69993813764864	22.160837203821718	27.548630681762386	28.59059397676725
15	25.162879643358632	27.690521313052958	24.399542414988364	22.747056628600046
16	22.55250935299836	26.343787743398895	27.16248195681419	23.941220946788558
17	22.688140103496696	27.140388259900433	27.20642386512043	22.96504777148244
18	22.736623493946325	25.0963530670961	28.455576940072074	23.711446498885497
19	23.7110782706036	25.292373255825375	29.60776323412445	21.388785239446577
20	23.238641384931118	25.618132542542643	28.965573110497942	22.1776529620283
21	22.570920767093156	26.171456907471597	29.362768683903024	21.894853641532222
22	22.865748878131168	26.115608951384043	28.72462907137737	22.294013099107417
23	22.26946454698102	26.26535511935506	28.89217293964002	22.5730073940239
24	22.37870560394348	26.247802904584688	29.010374218128614	22.36311727334322
25	22.53630730859494	25.933704180127453	28.804411865788154	22.725576645489447
26	22.18882255324581	26.47180844273805	29.24248077848369	22.096888225532457
27	22.51458183996308	26.617013128565674	29.03185420123921	21.83655083023203
28	22.29695892536258	26.85758893940435	28.80220249609678	22.043249639136285
29	22.092346743389076	26.941054016634098	29.161225070945317	21.80537416903151
30	22.116404324472942	27.06023723720775	28.972078476811436	21.85127996150787
31	21.9389182925991	26.494270367933698	29.359577372126594	22.207233967340606
32	21.61475466177005	27.11620793605593	28.8333791572973	22.435658244876716
33	21.893257985644006	26.800513555710488	28.697011950235172	22.609216508410334
34	22.01587800351535	26.952591836133504	28.820000196388417	22.211529963962725
35	21.636111902120014	27.07472088296232	28.87670735180039	22.412459863117274
36	21.592783707616924	26.740246860240184	29.003009652490697	22.663959779652195
37	21.389644438771	26.30868331385815	29.34337532772317	22.95829691964768
38	21.296973654493858	26.41424208800165	29.51631987745363	22.772464380050863
39	20.762674417462858	26.245102563850786	30.255599524740028	22.736623493946325
40	21.169934897239763	26.319116448511863	30.003240408880682	22.507708245367688
41	20.885908149137364	26.268423688370863	30.0746766955685	22.77099146692328
42	20.404020070896216	26.604002395938686	29.890930782902426	23.101046750262668
43	20.418012745608262	26.18103084280089	29.724614342246092	23.67634206934475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1101.0
1	828.0
2	555.0
3	1113.5
4	1672.0
5	1672.0
6	2092.5
7	2513.0
8	2585.0
9	2657.0
10	3871.5
11	5086.0
12	5086.0
13	8351.0
14	11616.0
15	15274.5
16	18933.0
17	16835.0
18	14737.0
19	14737.0
20	15977.0
21	17217.0
22	12754.5
23	8292.0
24	9033.0
25	9774.0
26	9774.0
27	11238.5
28	12703.0
29	13999.0
30	15295.0
31	16852.0
32	18409.0
33	18409.0
34	20613.0
35	22817.0
36	25963.0
37	29109.0
38	32543.0
39	35977.0
40	35977.0
41	39797.0
42	43617.0
43	47901.5
44	52186.0
45	56153.0
46	60120.0
47	60120.0
48	63341.5
49	66563.0
50	67303.0
51	68043.0
52	67000.0
53	65957.0
54	65957.0
55	61724.5
56	57492.0
57	52777.5
58	48063.0
59	43610.5
60	39158.0
61	39158.0
62	34263.5
63	29369.0
64	24846.5
65	20324.0
66	16759.0
67	13194.0
68	13194.0
69	10916.5
70	8639.0
71	7029.0
72	5419.0
73	4440.0
74	3461.0
75	3461.0
76	2784.5
77	2108.0
78	1657.0
79	1206.0
80	944.5
81	683.0
82	683.0
83	532.5
84	382.0
85	277.5
86	173.0
87	122.0
88	71.0
89	71.0
90	45.0
91	19.0
92	10.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	814712.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.3184418311495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.52224815844008	59.11065177912671
2	7.5838818426285135	10.36237981039854
3	2.341082902188146	4.798174082251185
4	1.1280118813424602	3.082560564013616
5	0.6385357003866615	2.181188205198922
6	0.4153614226114775	1.702610711375144
7	0.28366315195941433	1.356559718274584
8	0.2003338129236877	1.0949195156031477
9	0.15058990906504074	0.925926114853627
>10	0.6892934730477417	7.951794372674029
>50	0.028631549018563577	1.3283847466930538
>100	0.01620546741867122	2.034294055219269
>500	9.003037373262149E-4	0.40926989697813754
>1k	7.202429898609719E-4	0.9136301547510152
>5k	5.40182242395729E-4	2.7476562725890235
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8680	1.0654071622855685	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7839	0.962180500594075	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5817	0.7139946385962156	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3845	0.4719459146299551	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1462	0.17944991604395172	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1074	0.1318257249187443	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1046	0.1283889276210489	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	845	0.10371763273402135	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	840	0.10310391893086146	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.2274276063197793E-4	0.0	0.0	0.0	0.0
8	1.2274276063197793E-4	0.0	0.0	0.0	0.0
9	2.4548552126395587E-4	0.0	0.0	0.0	0.0
10	3.682282818959338E-4	0.0	0.0	0.0	0.0
11	3.682282818959338E-4	0.0	0.0	0.0	0.0
12	3.682282818959338E-4	1.2274276063197793E-4	0.0	1.2274276063197793E-4	0.0
13	3.682282818959338E-4	1.2274276063197793E-4	0.0	1.2274276063197793E-4	0.0
14	3.682282818959338E-4	1.2274276063197793E-4	0.0	1.2274276063197793E-4	0.0
15	4.909710425279117E-4	1.2274276063197793E-4	0.0	4.909710425279117E-4	0.0
16	4.909710425279117E-4	1.2274276063197793E-4	0.0	8.591993244238455E-4	0.0
17	4.909710425279117E-4	1.2274276063197793E-4	0.0	0.0011046848456878013	0.0
18	6.137138031598896E-4	1.2274276063197793E-4	0.0	0.0011046848456878013	0.0
19	6.137138031598896E-4	1.2274276063197793E-4	0.0	0.002086626930743625	0.0
20	7.364565637918676E-4	1.2274276063197793E-4	0.0	0.0025775979732715363	1.2274276063197793E-4
21	7.364565637918676E-4	1.2274276063197793E-4	0.0	0.0038050255795913156	1.2274276063197793E-4
22	7.364565637918676E-4	1.2274276063197793E-4	0.0	0.006014395270966919	1.2274276063197793E-4
23	7.364565637918676E-4	1.2274276063197793E-4	0.0	0.007364565637918675	3.682282818959338E-4
24	7.364565637918676E-4	1.2274276063197793E-4	0.0	0.009942163611190212	3.682282818959338E-4
25	7.364565637918676E-4	1.2274276063197793E-4	0.0	0.01190604778130186	3.682282818959338E-4
26	7.364565637918676E-4	1.2274276063197793E-4	0.0	0.01706124372784493	3.682282818959338E-4
27	7.364565637918676E-4	1.2274276063197793E-4	0.0	0.038172998556545136	3.682282818959338E-4
28	7.364565637918676E-4	1.2274276063197793E-4	0.0	0.10384037549465333	3.682282818959338E-4
29	7.364565637918676E-4	1.2274276063197793E-4	0.0	0.23321124520075806	3.682282818959338E-4
30	7.364565637918676E-4	1.2274276063197793E-4	0.0	0.3882353518789462	3.682282818959338E-4
31	7.364565637918676E-4	1.2274276063197793E-4	0.0	0.7365793065524995	3.682282818959338E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGA	45	0.003824866	20.555555	6
TATAGGG	60	9.233668E-4	18.5	2
CCCTATA	75	2.0663353E-4	17.266666	2
TCTAACG	65	0.0015794003	17.076923	2
CCCCTAT	70	0.0025916242	15.857143	1
GTCCTAT	70	0.0025916242	15.857143	1
GTATAGG	95	7.0556416E-5	15.578948	1
CTTATAC	660	0.0	15.416667	37
CCTATGC	85	5.3617713E-4	15.235293	3
GTAGTAA	85	5.3617713E-4	15.235293	1
ACCCGTT	185	3.0377123E-10	15.0	7
CTTAATA	75	0.0041030417	14.8	2
CGCTCAC	75	0.0041030417	14.8	10
GGTCTAG	100	1.09274326E-4	14.799999	1
TAGAGCA	90	8.270368E-4	14.388888	4
TAGGGTG	90	8.270368E-4	14.388888	5
CTAACAC	105	1.6546977E-4	14.095238	3
TATACTA	105	1.6546977E-4	14.095238	5
TATACAC	290	0.0	14.034483	37
ATACCCG	185	4.8876245E-9	14.0	5
>>END_MODULE