FastQCFastQC Report
Thu 9 Feb 2017
SRR2727086.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727086.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2496450
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT99150.3971639728414349No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT89200.35730737647459393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT68150.27298764245228224No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49220.19715996715335776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47350.015.9017961
ATACCGT1403.4782715E-815.8571436
TATTCCG1059.351796E-615.8571425
CTTATAC19900.014.31658337
TAGTACT2551.8189894E-1213.7843134
GTAATAC1907.1377144E-913.6315793
TACTTAC7200.013.61805531
TACGGAT6800.013.602941527
GTTACGG5450.013.23853225
CGTTACG3100.013.12903224
GCGCGAC850.00940876813.05882425
CGCCGAT850.00940876813.05882419
TCGTTAC4050.012.79012323
CATTACG3950.012.6455724
CTAGACA2805.456968E-1212.5535724
TCTTATA29950.012.47746437
CTCTTAT45650.012.40087737
GTACTAA1050.002645065512.3333331
GGACCGT1501.9455794E-512.3333336
GTATCAA62550.012.0671462
Produced by FastQC (version 0.11.5)