##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727086.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2496450
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.634734523022694	31.0	31.0	34.0	30.0	34.0
2	31.889154599531334	31.0	31.0	34.0	30.0	34.0
3	32.00089006389072	33.0	31.0	34.0	30.0	34.0
4	35.72586152336318	37.0	35.0	37.0	35.0	37.0
5	35.34004486370646	37.0	35.0	37.0	33.0	37.0
6	35.356672875483184	37.0	35.0	37.0	33.0	37.0
7	35.30974143283463	37.0	35.0	37.0	32.0	37.0
8	35.26144965851509	37.0	35.0	37.0	32.0	37.0
9	37.09306415109455	39.0	37.0	39.0	33.0	39.0
10	36.87036271505538	39.0	37.0	39.0	33.0	39.0
11	36.97726251276813	39.0	37.0	39.0	33.0	39.0
12	36.86416110877446	39.0	37.0	39.0	33.0	39.0
13	36.99200705001102	39.0	37.0	39.0	33.0	39.0
14	38.043831841214526	40.0	37.0	41.0	33.0	41.0
15	38.045737347032784	40.0	37.0	41.0	33.0	41.0
16	37.98842235975085	40.0	37.0	41.0	33.0	41.0
17	37.90361313064552	40.0	37.0	41.0	33.0	41.0
18	37.92198882413027	40.0	37.0	41.0	33.0	41.0
19	37.96822367762223	40.0	37.0	41.0	33.0	41.0
20	37.856841915520036	39.0	37.0	41.0	33.0	41.0
21	37.846883774960446	39.0	37.0	41.0	33.0	41.0
22	37.75565943639969	39.0	37.0	41.0	32.0	41.0
23	37.69223537423141	39.0	37.0	41.0	32.0	41.0
24	37.60354503394821	39.0	37.0	41.0	32.0	41.0
25	37.573127841534976	39.0	37.0	41.0	32.0	41.0
26	37.44078431372549	39.0	36.0	40.0	32.0	41.0
27	37.25408680326063	39.0	36.0	40.0	31.0	41.0
28	37.14537643453704	39.0	36.0	40.0	31.0	41.0
29	37.06588395521641	39.0	36.0	40.0	31.0	41.0
30	37.01246129503895	39.0	36.0	40.0	31.0	41.0
31	36.96119810130385	39.0	36.0	40.0	31.0	41.0
32	36.847231468685536	39.0	36.0	40.0	31.0	41.0
33	36.811052093973444	39.0	35.0	40.0	30.0	41.0
34	36.79370105549881	39.0	35.0	40.0	30.0	41.0
35	36.692931562819204	39.0	35.0	40.0	30.0	41.0
36	36.60984037333013	39.0	35.0	40.0	30.0	41.0
37	36.471565222616114	38.0	35.0	40.0	30.0	41.0
38	36.420700194275874	38.0	35.0	40.0	30.0	41.0
39	36.32297262112199	38.0	35.0	40.0	30.0	41.0
40	36.25536541889483	38.0	35.0	40.0	30.0	41.0
41	36.197572553025296	38.0	35.0	40.0	30.0	41.0
42	36.12567165374832	38.0	35.0	40.0	29.0	41.0
43	34.78989845580725	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	6.0
11	4.0
12	8.0
13	2.0
14	5.0
15	3.0
16	11.0
17	17.0
18	49.0
19	132.0
20	277.0
21	657.0
22	1211.0
23	2358.0
24	4137.0
25	6839.0
26	11070.0
27	16725.0
28	25041.0
29	35848.0
30	49794.0
31	65963.0
32	87084.0
33	115118.0
34	152893.0
35	205805.0
36	286068.0
37	408828.0
38	611635.0
39	408860.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.49554367201426	18.574415670251756	12.859700775100643	26.070339882633338
2	18.446153538023992	20.790682769532737	35.696208616235054	25.066955076208213
3	20.152015862524784	22.39956738568768	30.007170181657955	27.441246570129586
4	15.168379098319615	15.60435818862785	33.75749564381422	35.469767069238316
5	15.632077550121174	35.38656892787759	33.734583108013375	15.246770413987862
6	35.43259428388311	33.782851649342064	15.095956257886197	15.688597808888622
7	29.034709287187805	30.239740431412603	21.30725630395161	19.418293977447977
8	26.289491077329807	34.86627010354704	19.685913997876987	19.15832482124617
9	26.586673075767592	14.847563540227124	19.735464359390335	38.83029902461496
10	17.40591640128983	26.49998998577981	32.92146848524906	23.172625127681307
11	35.43836247471409	21.846221634721307	21.36297542510365	21.352440465460955
12	21.35167938472631	24.704480362114204	30.33667808287769	23.6071621702818
13	30.84167517875383	19.762542810791324	24.38650884255643	25.009273167898417
14	22.142121812974423	20.79849386128302	25.84453924572894	31.21484508001362
15	25.293837248893432	27.363976847122917	22.815558092491337	24.526627811492318
16	24.203248613030503	26.152977227663282	25.10653127440966	24.537242884896553
17	23.91808367882393	26.231168258927678	25.673816819884237	24.17693124236416
18	23.88119129163412	25.423461315067396	26.488373490356302	24.206973902942178
19	24.91602074946424	25.218450199282984	26.826533677822507	23.03899537343027
20	24.310801337899818	24.948186424723108	26.7919645897174	23.949047647659675
21	23.893689038434577	25.411043682028478	27.13060545975285	23.564661819784092
22	24.54573494361994	24.989204670632297	26.290692783752927	24.174367601994835
23	23.895771996234654	25.28486450760079	26.551142622523983	24.26822087364057
24	23.870375933826033	25.203669210278594	26.550461655550883	24.37549320034449
25	24.24983476536682	25.054657613811614	26.32009453423862	24.37541308658295
26	23.86368643473733	25.327685313144666	26.719301407999357	24.08932684411865
27	24.314166115884557	25.653788379498888	26.291654148891425	23.74039135572513
28	23.897774840273186	25.73522401810571	26.168198842356144	24.19880229926496
29	23.786897394299906	25.91067315588135	26.64936209417373	23.653067355645017
30	24.342886899397143	25.473332131626908	26.300466662660977	23.88331430631497
31	24.066173967033187	25.143343547837933	26.669470648320615	24.121011836808268
32	23.60423801798554	25.649261951971802	26.503875503214562	24.242624526828095
33	23.667968515291715	25.060666145927218	26.593322517975526	24.678042820805544
34	24.019427587173787	25.080454245027937	26.963568266939053	23.93654990085922
35	23.782290853011276	25.1261591459873	26.82965811452262	24.2618918864788
36	23.545194175729538	24.81207314386429	27.209076889182636	24.433655791223536
37	23.329207474613952	24.46790442428248	27.677502052915138	24.525386048188427
38	23.40419395541669	24.308197640649723	27.84946624206373	24.438142161869855
39	22.868072663181717	24.398285565503013	28.402852049910877	24.330789721404393
40	23.294678443389614	24.145726932243786	28.408700354503395	24.150894269863205
41	22.603617136333593	24.397244086602974	28.508922670191673	24.490216106871756
42	22.327505057181195	24.653327725370026	28.461455266478396	24.55771195097038
43	22.094494181738067	24.402932163672414	28.310440825972883	25.192132828616636
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1925.0
1	1651.5
2	1378.0
3	2374.5
4	3371.0
5	3371.0
6	4009.5
7	4648.0
8	4251.5
9	3855.0
10	5444.5
11	7034.0
12	7034.0
13	11072.5
14	15111.0
15	18442.5
16	21774.0
17	19906.5
18	18039.0
19	18039.0
20	20735.5
21	23432.0
22	20108.0
23	16784.0
24	19512.5
25	22241.0
26	22241.0
27	26669.0
28	31097.0
29	35709.5
30	40322.0
31	46838.0
32	53354.0
33	53354.0
34	61223.0
35	69092.0
36	79226.0
37	89360.0
38	100992.5
39	112625.0
40	112625.0
41	125145.5
42	137666.0
43	150987.5
44	164309.0
45	177523.5
46	190738.0
47	190738.0
48	203268.5
49	215799.0
50	220693.0
51	225587.0
52	223378.5
53	221170.0
54	221170.0
55	208802.5
56	196435.0
57	183069.5
58	169704.0
59	154424.0
60	139144.0
61	139144.0
62	121564.5
63	103985.0
64	87975.5
65	71966.0
66	59533.5
67	47101.0
68	47101.0
69	38406.0
70	29711.0
71	24225.5
72	18740.0
73	15372.0
74	12004.0
75	12004.0
76	9686.5
77	7369.0
78	5985.5
79	4602.0
80	3554.0
81	2506.0
82	2506.0
83	1934.0
84	1362.0
85	1046.5
86	731.0
87	500.5
88	270.0
89	270.0
90	177.5
91	85.0
92	50.5
93	16.0
94	10.0
95	4.0
96	4.0
97	3.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2496450.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.87306403997243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.40420413571977	53.2728207196376
2	8.887654766313277	11.353634841077882
3	2.871113789286367	5.501605047874081
4	1.4089332062341158	3.5997152363934144
5	0.8223818009199468	2.626402271773381
6	0.5286759162933145	2.0260890394676387
7	0.3824304394140265	1.7098902763268833
8	0.2821604008865358	1.4417959484295997
9	0.2145037952572665	1.2330913186156063
>10	1.1431990396986673	12.629911066308214
>50	0.042637907384991354	1.8123706729157256
>100	0.01122473836461514	1.1273812487388113
>500	2.514469220103398E-4	0.09114438937381646
>1k	4.400321135132654E-4	0.5442564748673953
>5k	1.8858519150568518E-4	1.0298914481998294
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9915	0.3971639728414349	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8920	0.35730737647459393	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6815	0.27298764245228224	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4922	0.19715996715335776	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.0056880770694385E-5
2	0.0	0.0	0.0	0.0	4.0056880770694385E-5
3	0.0	0.0	0.0	0.0	4.0056880770694385E-5
4	0.0	0.0	0.0	0.0	4.0056880770694385E-5
5	0.0	0.0	0.0	0.0	8.011376154138877E-5
6	0.0	0.0	0.0	0.0	1.2017064231208316E-4
7	0.0	0.0	0.0	0.0	1.2017064231208316E-4
8	0.0	0.0	0.0	0.0	1.6022752308277754E-4
9	0.0	0.0	0.0	4.0056880770694385E-5	2.0028440385347194E-4
10	0.0	0.0	0.0	4.0056880770694385E-5	2.0028440385347194E-4
11	0.0	0.0	0.0	4.0056880770694385E-5	2.0028440385347194E-4
12	0.0	0.0	0.0	8.011376154138877E-5	2.0028440385347194E-4
13	0.0	0.0	0.0	8.011376154138877E-5	2.0028440385347194E-4
14	0.0	0.0	0.0	8.011376154138877E-5	2.403412846241663E-4
15	0.0	0.0	0.0	8.011376154138877E-5	2.803981653948607E-4
16	0.0	0.0	0.0	2.0028440385347194E-4	4.005688077069439E-4
17	0.0	0.0	0.0	2.403412846241663E-4	4.005688077069439E-4
18	0.0	0.0	0.0	2.803981653948607E-4	4.406256884776382E-4
19	4.0056880770694385E-5	0.0	0.0	4.005688077069439E-4	4.806825692483326E-4
20	4.0056880770694385E-5	0.0	0.0	7.21023853872499E-4	4.806825692483326E-4
21	4.0056880770694385E-5	0.0	0.0	0.001361933946203609	4.806825692483326E-4
22	4.0056880770694385E-5	4.0056880770694385E-5	0.0	0.0022431853231588855	4.806825692483326E-4
23	4.0056880770694385E-5	4.0056880770694385E-5	0.0	0.00292415229626069	6.409100923311102E-4
24	4.0056880770694385E-5	4.0056880770694385E-5	0.0	0.004646598169400549	6.409100923311102E-4
25	4.0056880770694385E-5	4.0056880770694385E-5	0.0	0.005928418354062769	6.409100923311102E-4
26	4.0056880770694385E-5	4.0056880770694385E-5	0.0	0.009092911934947625	6.809669731018045E-4
27	4.0056880770694385E-5	4.0056880770694385E-5	0.0	0.030523343147269122	6.809669731018045E-4
28	4.0056880770694385E-5	4.0056880770694385E-5	0.0	0.09028820925714515	6.809669731018045E-4
29	4.0056880770694385E-5	4.0056880770694385E-5	0.0	0.1819383524604939	6.809669731018045E-4
30	4.0056880770694385E-5	4.0056880770694385E-5	0.0	0.29445813054537445	6.809669731018045E-4
31	4.0056880770694385E-5	4.0056880770694385E-5	0.0	0.6044583308297783	7.21023853872499E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4735	0.0	15.901796	1
ATACCGT	140	3.4782715E-8	15.857143	6
TATTCCG	105	9.351796E-6	15.857142	5
CTTATAC	1990	0.0	14.316583	37
TAGTACT	255	1.8189894E-12	13.784313	4
GTAATAC	190	7.1377144E-9	13.631579	3
TACTTAC	720	0.0	13.618055	31
TACGGAT	680	0.0	13.6029415	27
GTTACGG	545	0.0	13.238532	25
CGTTACG	310	0.0	13.129032	24
GCGCGAC	85	0.009408768	13.058824	25
CGCCGAT	85	0.009408768	13.058824	19
TCGTTAC	405	0.0	12.790123	23
CATTACG	395	0.0	12.64557	24
CTAGACA	280	5.456968E-12	12.553572	4
TCTTATA	2995	0.0	12.477464	37
CTCTTAT	4565	0.0	12.400877	37
GTACTAA	105	0.0026450655	12.333333	1
GGACCGT	150	1.9455794E-5	12.333333	6
GTATCAA	6255	0.0	12.067146	2
>>END_MODULE