FastQCFastQC Report
Thu 9 Feb 2017
SRR2727085.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727085.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2081882
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT93150.44743169881866507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT84240.4046338841490536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61310.2944931557119952No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41450.19909870011845052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCCG1352.2220775E-816.4444455
GGTATCA45150.015.8571431
CTTATAC13450.015.40520637
TCTATAC2009.840733E-1013.8749993
CTCTTAT31850.013.70800637
GCGCTAT1254.8480717E-513.321
TGCGACG850.0094080813.05882422
GTTATAT1703.7351128E-713.0588241
ACCCGTT2152.903107E-912.9069777
GATCTAT1307.003137E-512.8076921
GGGTATA2753.6379788E-1212.7818181
GTACTAA1501.9452138E-512.3333331
TACCCGT1705.3137737E-611.9705896
CTACACT4500.011.9222224
GTAATAC2351.0888471E-811.8085113
TCTTATA20200.011.72277337
GTATTAC1757.340055E-611.6285711
TCTCTTA47950.011.57455637
TATACAC6100.011.5245937
GTATCAA62150.011.5197112
Produced by FastQC (version 0.11.5)