##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727085.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2081882
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68688379072397	31.0	31.0	34.0	30.0	34.0
2	31.930877446464304	31.0	31.0	34.0	30.0	34.0
3	32.04043168632997	33.0	31.0	34.0	30.0	34.0
4	35.759243319265934	37.0	35.0	37.0	35.0	37.0
5	35.38074444180794	37.0	35.0	37.0	33.0	37.0
6	35.39560407362185	37.0	35.0	37.0	33.0	37.0
7	35.34994442528443	37.0	35.0	37.0	33.0	37.0
8	35.3088465148361	37.0	35.0	37.0	32.0	37.0
9	37.15175740027533	39.0	37.0	39.0	33.0	39.0
10	36.92766016517747	39.0	37.0	39.0	33.0	39.0
11	37.039610314129234	39.0	37.0	39.0	33.0	39.0
12	36.92398608566672	39.0	37.0	39.0	33.0	39.0
13	37.052735937963824	39.0	37.0	39.0	33.0	39.0
14	38.10941926583735	40.0	37.0	41.0	33.0	41.0
15	38.10991208915779	40.0	37.0	41.0	33.0	41.0
16	38.05875116841396	40.0	37.0	41.0	33.0	41.0
17	37.96586357920382	40.0	37.0	41.0	33.0	41.0
18	37.99209321181508	40.0	37.0	41.0	33.0	41.0
19	38.034580249985346	40.0	37.0	41.0	33.0	41.0
20	37.92247255127812	40.0	37.0	41.0	33.0	41.0
21	37.91636509658088	40.0	37.0	41.0	33.0	41.0
22	37.820893307113465	39.0	37.0	41.0	33.0	41.0
23	37.769290958853574	39.0	37.0	41.0	33.0	41.0
24	37.670971745756965	39.0	37.0	41.0	32.0	41.0
25	37.65051477461259	39.0	37.0	41.0	32.0	41.0
26	37.517611949188286	39.0	37.0	40.0	32.0	41.0
27	37.33891738340598	39.0	36.0	40.0	32.0	41.0
28	37.23846548459519	39.0	36.0	40.0	31.0	41.0
29	37.154441029799	39.0	36.0	40.0	31.0	41.0
30	37.09001422751145	39.0	36.0	40.0	31.0	41.0
31	37.041176204991444	39.0	36.0	40.0	31.0	41.0
32	36.93677499493247	39.0	36.0	40.0	31.0	41.0
33	36.89023441290141	39.0	35.0	40.0	31.0	41.0
34	36.86315458801219	39.0	35.0	40.0	31.0	41.0
35	36.76278194441375	39.0	35.0	40.0	30.0	41.0
36	36.67639856629722	39.0	35.0	40.0	30.0	41.0
37	36.533970225017555	39.0	35.0	40.0	30.0	41.0
38	36.47683105958935	39.0	35.0	40.0	30.0	41.0
39	36.376919537226414	38.0	35.0	40.0	30.0	41.0
40	36.2985231631764	38.0	35.0	40.0	30.0	41.0
41	36.23017971239484	38.0	35.0	40.0	30.0	41.0
42	36.15489062300361	38.0	35.0	40.0	30.0	41.0
43	34.81976692242884	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	9.0
12	4.0
13	2.0
14	2.0
15	6.0
16	3.0
17	13.0
18	46.0
19	102.0
20	234.0
21	479.0
22	1013.0
23	1866.0
24	3202.0
25	5573.0
26	8644.0
27	13248.0
28	19700.0
29	28539.0
30	39431.0
31	53266.0
32	70369.0
33	93745.0
34	124551.0
35	170628.0
36	237893.0
37	341504.0
38	514855.0
39	352951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.711594605265816	18.611381432761316	12.807882483253133	25.869141478719737
2	18.595194156056877	20.819863950022143	35.43020209598815	25.154739797932834
3	20.23399981363017	22.362026281989085	29.975810348521193	27.428163555859552
4	15.15777551273319	15.67773773921865	33.715407501481835	35.44907924656633
5	15.657371551317508	35.248059207966634	33.73529335476266	15.359275885953192
6	35.36266704837258	33.766082803924526	15.075302058425983	15.795948089276916
7	29.060340595672567	30.07125283757677	21.27954418165871	19.58886238509195
8	26.35730555334068	34.548019532327004	19.62315827698208	19.471516637350245
9	26.298224395042563	14.887923523043092	19.904634364483673	38.90921771743067
10	17.546863847230533	26.415762276632393	32.89120132649209	23.146172549644987
11	35.320253501399215	21.78634523954768	21.45875702849633	21.434644230556778
12	21.421483061960284	24.97989799614003	30.120775336930723	23.477843604968964
13	30.84627274744678	19.74175289473659	24.394802395140548	25.017171962676077
14	22.19097912369673	20.934615890814175	25.84229077344441	31.032114212044682
15	25.20767267309098	27.41408975148447	22.84245696922304	24.535780606201506
16	24.271596565031064	25.96703367433889	25.04238953024235	24.718980230387697
17	23.841552979467618	26.279971679470787	25.790414634450944	24.088060706610655
18	23.901354639696198	25.15651703602798	26.6540082483061	24.288120075969726
19	24.822684474912606	25.07404358172077	27.048699205814742	23.054572737551887
20	24.325922410588113	25.076301154436226	26.878516649838947	23.719259785136718
21	23.932768523864464	25.39010376188468	27.037411342237455	23.6397163720134
22	24.316363751643948	25.126448088796575	26.537286935570798	24.01990122398868
23	23.914852042526906	25.231449236796323	26.539784675596405	24.313914045080367
24	23.882429455655988	25.320022940781467	26.65021360480565	24.147333998756896
25	24.221689797980865	25.081440734873546	26.46086569747949	24.2360037696661
26	23.887136734935023	25.492126835238498	26.6656323461176	23.955104083708875
27	24.164914245860235	25.648043452991093	26.411679432359758	23.775362868788914
28	23.928829779977924	25.617109903443136	26.47527573608879	23.978784580490153
29	23.800628469817216	25.829561906006198	26.7550706524193	23.614738971757284
30	23.95534425101903	25.70659624320687	26.523213131195718	23.814846374578387
31	23.922489362989833	25.158486407971246	26.81472821226179	24.104296016777127
32	23.45992712363141	25.690553066888516	26.671396361561317	24.178123447918757
33	23.643222814741662	25.28760035391055	26.63830130622197	24.430875525125824
34	23.91302677096973	25.20603953538193	26.922179066825112	23.958754626823232
35	23.684387491702218	25.373484184021954	26.895376395011823	24.046751929264
36	23.52616526777214	24.94199959459758	27.177813151754037	24.35402198587624
37	23.375820531615144	24.61959899744558	27.50861960476146	24.495960866177814
38	23.161303090184745	24.602547118424578	27.888900523660805	24.34724926772987
39	22.79735354837594	24.534483702726668	28.313276160704593	24.354886588192798
40	22.989487396499896	24.503886387412926	28.390081666492144	24.11654454959503
41	22.60992697953102	24.461857108135813	28.526977033280467	24.401238879052702
42	22.250924884311406	24.84060095625016	28.36361522891307	24.54485893052536
43	22.115374454459953	24.560469805685432	28.35285573341813	24.971300006436483
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1463.0
1	1178.0
2	893.0
3	1685.5
4	2478.0
5	2478.0
6	3022.0
7	3566.0
8	3310.5
9	3055.0
10	4397.0
11	5739.0
12	5739.0
13	9311.0
14	12883.0
15	16505.0
16	20127.0
17	18315.0
18	16503.0
19	16503.0
20	18785.0
21	21067.0
22	17905.0
23	14743.0
24	17086.5
25	19430.0
26	19430.0
27	23313.0
28	27196.0
29	30988.5
30	34781.0
31	40219.5
32	45658.0
33	45658.0
34	52417.5
35	59177.0
36	67360.0
37	75543.0
38	85347.5
39	95152.0
40	95152.0
41	105276.5
42	115401.0
43	125603.5
44	135806.0
45	146321.0
46	156836.0
47	156836.0
48	165972.5
49	175109.0
50	179342.5
51	183576.0
52	180899.0
53	178222.0
54	178222.0
55	170052.0
56	161882.0
57	151543.5
58	141205.0
59	129045.5
60	116886.0
61	116886.0
62	102264.0
63	87642.0
64	74411.0
65	61180.0
66	51002.0
67	40824.0
68	40824.0
69	33449.0
70	26074.0
71	21245.5
72	16417.0
73	13440.5
74	10464.0
75	10464.0
76	8481.0
77	6498.0
78	5205.5
79	3913.0
80	3065.5
81	2218.0
82	2218.0
83	1768.5
84	1319.0
85	969.5
86	620.0
87	434.5
88	249.0
89	249.0
90	158.5
91	68.0
92	40.5
93	13.0
94	9.0
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2081882.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.36494086009151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.3424713924104	55.13040654558865
2	8.437357231983245	11.030147129681003
3	2.6903224456169035	5.275583026569768
4	1.318212867033309	3.446596243785756
5	0.7430115955654528	2.4283454501249024
6	0.5127326033141412	2.010884177560176
7	0.3528464994400096	1.6144653398010573
8	0.2837342906419137	1.483702010223096
9	0.20299871285551196	1.1942098974427713
>10	1.0684994949147668	12.072705616036924
>50	0.03722443791327598	1.633240880592067
>100	0.00970702341857042	0.9457550677919945
>500	2.2035122312953346E-4	0.08843592226342267
>1k	4.4070244625891395E-4	0.49950452509684456
>5k	2.2035122312945697E-4	1.1460181674415302
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9315	0.44743169881866507	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8424	0.4046338841490536	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6131	0.2944931557119952	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4145	0.19909870011845052	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.8033462030989266E-5
2	4.8033462030989266E-5	0.0	0.0	0.0	4.8033462030989266E-5
3	4.8033462030989266E-5	0.0	0.0	0.0	4.8033462030989266E-5
4	4.8033462030989266E-5	0.0	0.0	4.8033462030989266E-5	4.8033462030989266E-5
5	4.8033462030989266E-5	0.0	0.0	4.8033462030989266E-5	4.8033462030989266E-5
6	4.8033462030989266E-5	0.0	0.0	4.8033462030989266E-5	9.606692406197853E-5
7	9.606692406197853E-5	0.0	0.0	4.8033462030989266E-5	9.606692406197853E-5
8	9.606692406197853E-5	0.0	0.0	4.8033462030989266E-5	1.9213384812395706E-4
9	1.441003860929678E-4	0.0	0.0	4.8033462030989266E-5	1.9213384812395706E-4
10	1.441003860929678E-4	0.0	0.0	4.8033462030989266E-5	1.9213384812395706E-4
11	1.441003860929678E-4	0.0	0.0	9.606692406197853E-5	1.9213384812395706E-4
12	1.441003860929678E-4	0.0	0.0	9.606692406197853E-5	1.9213384812395706E-4
13	1.441003860929678E-4	0.0	0.0	9.606692406197853E-5	1.9213384812395706E-4
14	1.441003860929678E-4	0.0	0.0	1.441003860929678E-4	2.882007721859356E-4
15	1.441003860929678E-4	0.0	0.0	1.441003860929678E-4	2.882007721859356E-4
16	1.9213384812395706E-4	0.0	0.0	2.882007721859356E-4	2.882007721859356E-4
17	1.9213384812395706E-4	0.0	0.0	3.842676962479141E-4	2.882007721859356E-4
18	1.9213384812395706E-4	0.0	0.0	4.323011582789034E-4	2.882007721859356E-4
19	1.9213384812395706E-4	0.0	0.0	6.244350064028605E-4	2.882007721859356E-4
20	1.9213384812395706E-4	0.0	0.0	8.646023165578068E-4	2.882007721859356E-4
21	1.9213384812395706E-4	0.0	0.0	0.0012969034748367102	2.882007721859356E-4
22	1.9213384812395706E-4	0.0	0.0	0.0019213384812395706	2.882007721859356E-4
23	1.9213384812395706E-4	0.0	0.0	0.0022575727154564955	2.882007721859356E-4
24	1.9213384812395706E-4	0.0	0.0	0.0031702084940452915	3.3623423421692486E-4
25	1.9213384812395706E-4	4.8033462030989266E-5	0.0	0.004274978120758045	3.3623423421692486E-4
26	1.9213384812395706E-4	4.8033462030989266E-5	0.0	0.008213722007299164	3.842676962479141E-4
27	1.9213384812395706E-4	4.8033462030989266E-5	0.0	0.02627430373095113	3.842676962479141E-4
28	1.9213384812395706E-4	4.8033462030989266E-5	0.0	0.07776617502817162	3.842676962479141E-4
29	1.9213384812395706E-4	4.8033462030989266E-5	0.0	0.1597592947150703	4.323011582789034E-4
30	1.9213384812395706E-4	4.8033462030989266E-5	0.0	0.2619745019170155	4.323011582789034E-4
31	1.9213384812395706E-4	4.8033462030989266E-5	0.0	0.5401362805384743	4.323011582789034E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCCG	135	2.2220775E-8	16.444445	5
GGTATCA	4515	0.0	15.857143	1
CTTATAC	1345	0.0	15.405206	37
TCTATAC	200	9.840733E-10	13.874999	3
CTCTTAT	3185	0.0	13.708006	37
GCGCTAT	125	4.8480717E-5	13.32	1
TGCGACG	85	0.00940808	13.058824	22
GTTATAT	170	3.7351128E-7	13.058824	1
ACCCGTT	215	2.903107E-9	12.906977	7
GATCTAT	130	7.003137E-5	12.807692	1
GGGTATA	275	3.6379788E-12	12.781818	1
GTACTAA	150	1.9452138E-5	12.333333	1
TACCCGT	170	5.3137737E-6	11.970589	6
CTACACT	450	0.0	11.922222	4
GTAATAC	235	1.0888471E-8	11.808511	3
TCTTATA	2020	0.0	11.722773	37
GTATTAC	175	7.340055E-6	11.628571	1
TCTCTTA	4795	0.0	11.574556	37
TATACAC	610	0.0	11.52459	37
GTATCAA	6215	0.0	11.519711	2
>>END_MODULE