FastQCFastQC Report
Thu 9 Feb 2017
SRR2727084.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727084.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3402692
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT159310.4681881286933992No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT140400.41261448288590324No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT104550.30725672496952416No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71880.21124450875953507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81600.015.7340691
CTTATAC26550.015.32956837
TCTTATA41550.013.31287637
CTCTTAT62550.012.83613137
ATTATAC4100.012.6341463
AACACCG2653.274181E-1112.5660375
TTCGTTA2102.8965587E-812.33333322
GTATCAA105550.012.1288492
TATACAC11100.011.66666737
TATACTA4600.011.6630435
TATACCG1757.343884E-611.62857155
TCGTTAC5950.011.50420123
TAAGACT5200.011.3846154
GTATAGA4100.011.2804871
TACGGAT9550.011.23560127
ATACGAC1655.15691E-511.2121213
ATACCGT2002.6611833E-611.16
TAGTACT2502.7186616E-811.14
TACACGC3553.6379788E-1210.9436625
AACGCAG115150.010.8606177
Produced by FastQC (version 0.11.5)