##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727084.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3402692
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08433616677619	33.0	31.0	34.0	30.0	34.0
2	32.308251819441786	34.0	31.0	34.0	30.0	34.0
3	32.41227739683756	34.0	31.0	34.0	30.0	34.0
4	36.01108710397532	37.0	35.0	37.0	35.0	37.0
5	35.7352701919539	37.0	35.0	37.0	35.0	37.0
6	35.75337350544804	37.0	35.0	37.0	33.0	37.0
7	35.72324559495834	37.0	35.0	37.0	33.0	37.0
8	35.69603654988462	37.0	35.0	37.0	33.0	37.0
9	37.52737097568631	39.0	37.0	39.0	35.0	39.0
10	37.360292380268326	39.0	37.0	39.0	34.0	39.0
11	37.44405400194905	39.0	37.0	39.0	35.0	39.0
12	37.360280330985	39.0	37.0	39.0	34.0	39.0
13	37.427005441573904	39.0	37.0	39.0	35.0	39.0
14	38.62960238540544	40.0	38.0	41.0	35.0	41.0
15	38.582907885873894	40.0	38.0	41.0	34.0	41.0
16	38.53399514266939	40.0	38.0	41.0	34.0	41.0
17	38.50709761565255	40.0	38.0	41.0	34.0	41.0
18	38.50890412649749	40.0	38.0	41.0	34.0	41.0
19	38.56381741280139	40.0	38.0	41.0	34.0	41.0
20	38.48851615132959	40.0	38.0	41.0	34.0	41.0
21	38.42981468790005	40.0	38.0	41.0	34.0	41.0
22	38.375877981315966	40.0	38.0	41.0	34.0	41.0
23	38.32008627286866	40.0	38.0	41.0	34.0	41.0
24	38.247345631047416	40.0	38.0	41.0	34.0	41.0
25	38.2213979402191	40.0	38.0	41.0	34.0	41.0
26	38.073820962931705	40.0	37.0	41.0	33.0	41.0
27	37.94428029336772	40.0	37.0	41.0	33.0	41.0
28	37.86770592225215	40.0	37.0	41.0	33.0	41.0
29	37.7838787642255	40.0	37.0	41.0	33.0	41.0
30	37.725867636565404	40.0	37.0	41.0	33.0	41.0
31	37.666396782312354	40.0	37.0	41.0	33.0	41.0
32	37.55165439599	39.0	37.0	41.0	33.0	41.0
33	37.46709458275977	39.0	37.0	41.0	32.0	41.0
34	37.421895076016284	39.0	36.0	41.0	32.0	41.0
35	37.338926062070854	39.0	36.0	41.0	32.0	41.0
36	37.21575534900015	39.0	36.0	41.0	31.0	41.0
37	37.13087843389881	39.0	36.0	41.0	31.0	41.0
38	37.087523349160016	39.0	36.0	41.0	31.0	41.0
39	36.97827808100175	39.0	36.0	41.0	31.0	41.0
40	36.89470395792508	39.0	36.0	41.0	31.0	41.0
41	36.830132730203026	39.0	36.0	40.0	31.0	41.0
42	36.676890238669856	39.0	35.0	40.0	30.0	41.0
43	35.42973827781063	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	1.0
13	5.0
14	4.0
15	5.0
16	16.0
17	30.0
18	86.0
19	170.0
20	352.0
21	696.0
22	1439.0
23	2562.0
24	4429.0
25	7023.0
26	11277.0
27	17027.0
28	25281.0
29	35491.0
30	48892.0
31	66260.0
32	87340.0
33	117417.0
34	159729.0
35	221319.0
36	323112.0
37	505030.0
38	891507.0
39	876187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.796233100145415	18.588782058440785	12.918389322336548	25.69659551907725
2	18.521364848772677	21.019240060516793	35.85687449819143	24.6025205925191
3	20.067052792318552	22.63302114913721	30.401605552309757	26.898320506234473
4	15.092961690332244	15.800254621928755	34.01318720589463	35.09359648184437
5	15.456585550499428	35.53753910139384	33.822867306238706	15.183008041868026
6	34.85610804621753	34.04821829304562	15.177218508169412	15.918455152567438
7	28.657545261222584	30.61567135667877	21.440847423157898	19.285935958940744
8	26.17057318146926	34.882616469548225	19.801851005027785	19.144959343954728
9	26.542278878017754	14.990660335992798	19.99687306403283	38.47018772195662
10	17.421794273475236	26.714730572146994	32.86018834499273	23.003286809385042
11	35.07287759221228	22.17441367011766	21.41522065470516	21.3374880829649
12	21.272421952971353	25.19840173603723	30.15856269095175	23.370613620039663
13	30.75168131585227	20.08741901999946	24.45851696245208	24.702382701696187
14	21.989824527168487	21.34924348133772	26.038236784287268	30.62269520720653
15	25.300967586840066	27.570758681655583	23.028325807919142	24.09994792358521
16	23.797040696013628	26.457169793798556	25.424281715770924	24.321507794416892
17	23.625147383307098	26.526379701718522	25.841216307558838	24.00725660741554
18	23.615125906194272	25.55776426429427	26.768393965718907	24.05871586379255
19	24.75234314478066	25.411174446585232	27.18747391770986	22.649008490924246
20	24.070324319685707	25.32159831098436	26.995743370249205	23.61233399908073
21	23.66038418992962	25.824729361340964	27.440479479188827	23.074406969540586
22	24.254266915724372	25.421107758210265	26.437126839572905	23.887498486492458
23	23.63878364541957	25.567344913968114	26.80692228388582	23.986949156726496
24	23.452460581210406	25.61945071725563	26.912015545338807	24.01607315619515
25	23.898754280434435	25.355806520249264	26.658304659957473	24.087134539358836
26	23.43891248458573	25.686574041964423	26.969705162853412	23.904808310596433
27	23.933432705634246	26.042321785221816	26.634999582683356	23.38924592646058
28	23.55100020807055	26.105242555012325	26.42475428278551	23.91900295413161
29	23.492869763116968	26.239342261950245	26.969852105333068	23.29793586959972
30	23.870129885396622	25.97546295697642	26.592151155614435	23.562256002012525
31	23.707611502892416	25.427396896339722	27.021399527197875	23.843592073569987
32	23.160867924572663	26.103773130215725	26.779943644620204	23.955415300591415
33	23.30460705817629	25.47494748275777	26.863054311116024	24.357391147949915
34	23.639312638346343	25.564112179415595	27.268821274449763	23.527753907788306
35	23.41872258788042	25.610310895020767	26.994332722444465	23.97663379465435
36	23.186759189488793	25.213184149491052	27.459464447560933	24.140592213459225
37	22.9585869070724	25.028683172029677	27.782091355902917	24.23063856499501
38	22.989944432231894	24.73347573039229	28.08232423034468	24.19425560703114
39	22.463596470088977	24.771239947664967	28.685728828821418	24.079434753424643
40	22.874447643218957	24.503275641756588	28.721053800931735	23.901222914092724
41	22.302283015917983	24.685983920966105	28.844749980309707	24.1669830828062
42	21.927638469776284	25.02974115788323	28.72831275942695	24.31430761291354
43	21.723829250487555	24.728420909091977	28.601325068504586	24.946424771915883
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2583.0
1	2225.0
2	1867.0
3	3217.5
4	4568.0
5	4568.0
6	5452.5
7	6337.0
8	5937.5
9	5538.0
10	7850.0
11	10162.0
12	10162.0
13	16402.5
14	22643.0
15	28249.5
16	33856.0
17	31109.0
18	28362.0
19	28362.0
20	32897.5
21	37433.0
22	32183.5
23	26934.0
24	31429.5
25	35925.0
26	35925.0
27	42611.0
28	49297.0
29	56001.0
30	62705.0
31	71769.0
32	80833.0
33	80833.0
34	92093.5
35	103354.0
36	115908.5
37	128463.0
38	143089.0
39	157715.0
40	157715.0
41	173959.5
42	190204.0
43	207300.5
44	224397.0
45	240165.5
46	255934.0
47	255934.0
48	271481.0
49	287028.0
50	293745.0
51	300462.0
52	294957.5
53	289453.0
54	289453.0
55	273171.0
56	256889.0
57	238520.0
58	220151.0
59	200171.0
60	180191.0
61	180191.0
62	157672.5
63	135154.0
64	114015.5
65	92877.0
66	77497.0
67	62117.0
68	62117.0
69	50944.0
70	39771.0
71	32928.0
72	26085.0
73	21346.5
74	16608.0
75	16608.0
76	13705.5
77	10803.0
78	8769.0
79	6735.0
80	5472.5
81	4210.0
82	4210.0
83	3393.0
84	2576.0
85	1997.0
86	1418.0
87	1087.0
88	756.0
89	756.0
90	482.0
91	208.0
92	137.0
93	66.0
94	41.0
95	16.0
96	16.0
97	10.5
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3402692.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.799620821632374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.756192118797	49.487889093462215
2	9.087472125621927	10.868547746786895
3	2.983066283793717	5.351586979699807
4	1.4095867005206584	3.3717100082540497
5	0.8585012871296451	2.566902572261806
6	0.5531922466567063	1.984841195492278
7	0.3850779387451024	1.6119260306613026
8	0.3043595009927162	1.4560466202260538
9	0.23113716465262465	1.2439723323612746
>10	1.3310460265175195	14.317349484256086
>50	0.07252281764247177	2.9358872506101914
>100	0.026419000034751085	2.6932239247169614
>500	9.347927270826529E-4	0.3522598420123685
>1k	2.951977008192326E-4	0.3569966502235534
>5k	4.9199616803205436E-5	0.21147947270536527
>10k+	1.475988504096163E-4	1.1893807962697684
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15931	0.4681881286933992	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14040	0.41261448288590324	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10455	0.30725672496952416	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7188	0.21124450875953507	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9388495932044394E-5	0.0	0.0	2.9388495932044394E-5	2.9388495932044394E-5
2	2.9388495932044394E-5	0.0	0.0	2.9388495932044394E-5	2.9388495932044394E-5
3	2.9388495932044394E-5	2.9388495932044394E-5	0.0	2.9388495932044394E-5	2.9388495932044394E-5
4	2.9388495932044394E-5	2.9388495932044394E-5	0.0	2.9388495932044394E-5	5.877699186408879E-5
5	2.9388495932044394E-5	5.877699186408879E-5	0.0	2.9388495932044394E-5	5.877699186408879E-5
6	2.9388495932044394E-5	5.877699186408879E-5	0.0	2.9388495932044394E-5	5.877699186408879E-5
7	2.9388495932044394E-5	5.877699186408879E-5	0.0	2.9388495932044394E-5	5.877699186408879E-5
8	2.9388495932044394E-5	5.877699186408879E-5	0.0	2.9388495932044394E-5	5.877699186408879E-5
9	2.9388495932044394E-5	5.877699186408879E-5	0.0	2.9388495932044394E-5	5.877699186408879E-5
10	8.816548779613318E-5	5.877699186408879E-5	0.0	2.9388495932044394E-5	8.816548779613318E-5
11	8.816548779613318E-5	5.877699186408879E-5	0.0	2.9388495932044394E-5	8.816548779613318E-5
12	8.816548779613318E-5	5.877699186408879E-5	0.0	5.877699186408879E-5	8.816548779613318E-5
13	8.816548779613318E-5	5.877699186408879E-5	0.0	1.1755398372817758E-4	1.4694247966022197E-4
14	8.816548779613318E-5	5.877699186408879E-5	0.0	1.7633097559226636E-4	2.3510796745635515E-4
15	8.816548779613318E-5	5.877699186408879E-5	0.0	2.3510796745635515E-4	2.6449646338839956E-4
16	1.4694247966022197E-4	5.877699186408879E-5	0.0	3.820504471165771E-4	2.6449646338839956E-4
17	1.4694247966022197E-4	5.877699186408879E-5	0.0	4.408274389806659E-4	2.6449646338839956E-4
18	1.7633097559226636E-4	8.816548779613318E-5	0.0	4.996044308447547E-4	2.6449646338839956E-4
19	1.7633097559226636E-4	8.816548779613318E-5	0.0	6.171584145729322E-4	2.6449646338839956E-4
20	1.7633097559226636E-4	8.816548779613318E-5	0.0	7.641008942331542E-4	2.6449646338839956E-4
21	1.7633097559226636E-4	8.816548779613318E-5	0.0	0.0013224823169419976	2.6449646338839956E-4
22	1.7633097559226636E-4	8.816548779613318E-5	0.0	0.0022041371949033297	2.6449646338839956E-4
23	1.7633097559226636E-4	8.816548779613318E-5	0.0	0.002791907113544217	3.820504471165771E-4
24	1.7633097559226636E-4	8.816548779613318E-5	0.0	0.003967446950825993	3.820504471165771E-4
25	1.7633097559226636E-4	8.816548779613318E-5	0.0	0.0054956487392923015	3.820504471165771E-4
26	1.7633097559226636E-4	8.816548779613318E-5	0.0	0.008934102763341495	4.114389430486215E-4
27	1.7633097559226636E-4	8.816548779613318E-5	0.0	0.030946086216442747	4.114389430486215E-4
28	1.7633097559226636E-4	8.816548779613318E-5	0.0	0.09142761084459011	4.408274389806659E-4
29	2.0571947152431074E-4	8.816548779613318E-5	0.0	0.18996723770473495	4.408274389806659E-4
30	2.0571947152431074E-4	8.816548779613318E-5	0.0	0.30302478155530976	4.408274389806659E-4
31	2.0571947152431074E-4	8.816548779613318E-5	0.0	0.6226834518081567	4.408274389806659E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8160	0.0	15.734069	1
CTTATAC	2655	0.0	15.329568	37
TCTTATA	4155	0.0	13.312876	37
CTCTTAT	6255	0.0	12.836131	37
ATTATAC	410	0.0	12.634146	3
AACACCG	265	3.274181E-11	12.566037	5
TTCGTTA	210	2.8965587E-8	12.333333	22
GTATCAA	10555	0.0	12.128849	2
TATACAC	1110	0.0	11.666667	37
TATACTA	460	0.0	11.663043	5
TATACCG	175	7.343884E-6	11.6285715	5
TCGTTAC	595	0.0	11.504201	23
TAAGACT	520	0.0	11.384615	4
GTATAGA	410	0.0	11.280487	1
TACGGAT	955	0.0	11.235601	27
ATACGAC	165	5.15691E-5	11.212121	3
ATACCGT	200	2.6611833E-6	11.1	6
TAGTACT	250	2.7186616E-8	11.1	4
TACACGC	355	3.6379788E-12	10.943662	5
AACGCAG	11515	0.0	10.860617	7
>>END_MODULE