FastQCFastQC Report
Thu 9 Feb 2017
SRR2727083.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727083.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4203714
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT202660.48209749759379444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT185630.44158570254779467No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT133540.3176714686108522No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91080.21666554860773116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC29650.014.849915537
GGTATCA113600.014.7544011
TATACAC13850.013.89169737
ATACCCG3350.013.805975
TCTTATA46550.013.79054737
CGCGTGA2307.927156E-912.06521810
TAACCGT1552.7237174E-511.9354846
CTCTTAT68400.011.81944537
TAAGACT5050.011.7227734
GTATCAA144500.011.5352952
TACCCGT3850.011.5324696
TATATCG1451.9405462E-411.4827595
TCTCTTA100050.011.37181437
TAATCCG1150.00522238311.260875
ACCCGTT4800.011.1770847
TCTACAC7050.011.0212763
ATTACGG4600.010.85869625
TATACCG2053.5787143E-610.8292685
CCGTTTA1200.007167707710.7916679
AACGCAG152150.010.7242867
Produced by FastQC (version 0.11.5)