##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727083.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4203714
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.954437671068963	33.0	31.0	34.0	30.0	34.0
2	32.184247072945496	33.0	31.0	34.0	30.0	34.0
3	32.289764717580695	34.0	31.0	34.0	30.0	34.0
4	35.912298505559605	37.0	35.0	37.0	35.0	37.0
5	35.62139764979254	37.0	35.0	37.0	33.0	37.0
6	35.64074435130458	37.0	35.0	37.0	33.0	37.0
7	35.60436199988867	37.0	35.0	37.0	33.0	37.0
8	35.57579844870512	37.0	35.0	37.0	33.0	37.0
9	37.39772758089632	39.0	37.0	39.0	34.0	39.0
10	37.224645634788665	39.0	37.0	39.0	34.0	39.0
11	37.305023129546875	39.0	37.0	39.0	34.0	39.0
12	37.2111558969045	39.0	37.0	39.0	34.0	39.0
13	37.2912755244529	39.0	37.0	39.0	34.0	39.0
14	38.46273985337727	40.0	38.0	41.0	34.0	41.0
15	38.413786713368225	40.0	38.0	41.0	34.0	41.0
16	38.36312770088546	40.0	38.0	41.0	34.0	41.0
17	38.333981331746166	40.0	38.0	41.0	34.0	41.0
18	38.33959446337215	40.0	38.0	41.0	34.0	41.0
19	38.38700943974781	40.0	38.0	41.0	34.0	41.0
20	38.31035008566234	40.0	38.0	41.0	34.0	41.0
21	38.2636437683439	40.0	38.0	41.0	34.0	41.0
22	38.19730005419017	40.0	38.0	41.0	34.0	41.0
23	38.13903324536351	40.0	38.0	41.0	33.0	41.0
24	38.0545205977381	40.0	37.0	41.0	33.0	41.0
25	38.02994851695429	40.0	37.0	41.0	33.0	41.0
26	37.87799693318813	40.0	37.0	41.0	33.0	41.0
27	37.741369417615	40.0	37.0	41.0	33.0	41.0
28	37.6563679165614	40.0	37.0	41.0	32.0	41.0
29	37.5570773844272	39.0	37.0	41.0	32.0	41.0
30	37.50213715776097	39.0	37.0	41.0	32.0	41.0
31	37.43917069524711	39.0	36.0	41.0	32.0	41.0
32	37.32100946924553	39.0	36.0	41.0	32.0	41.0
33	37.234880631746115	39.0	36.0	41.0	31.0	41.0
34	37.19205826086171	39.0	36.0	41.0	31.0	41.0
35	37.10405655570288	39.0	36.0	41.0	31.0	41.0
36	36.98436073434111	39.0	36.0	40.0	31.0	41.0
37	36.89292206843758	39.0	36.0	40.0	31.0	41.0
38	36.841931444432234	39.0	35.0	40.0	31.0	41.0
39	36.730534712875325	39.0	35.0	40.0	30.0	41.0
40	36.63974951673686	39.0	35.0	40.0	30.0	41.0
41	36.56893380472601	39.0	35.0	40.0	30.0	41.0
42	36.41864931819815	39.0	35.0	40.0	30.0	41.0
43	35.14806954041117	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	6.0
11	13.0
12	11.0
13	6.0
14	5.0
15	9.0
16	22.0
17	45.0
18	92.0
19	240.0
20	483.0
21	1066.0
22	2097.0
23	3766.0
24	6481.0
25	10549.0
26	16704.0
27	25129.0
28	35990.0
29	51187.0
30	69612.0
31	91843.0
32	120563.0
33	159907.0
34	212093.0
35	290133.0
36	412246.0
37	631785.0
38	1076141.0
39	985486.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.092394011581185	18.3689708671903	12.801346618728104	25.737288502500412
2	18.800898443614386	20.759785275592012	35.3108227629187	25.128493517874904
3	20.404409053517913	22.17986285460904	30.015457759495533	27.400270332377513
4	15.34117211589561	15.577368013142664	33.40369968080607	35.67776019015565
5	15.806950710728657	35.18543364272641	33.48764925492077	15.519966391624168
6	35.56968908921967	33.48536556007378	14.901180242043107	16.043765108663436
7	29.2314129838519	30.04892816209666	21.088756275997845	19.630902578053597
8	26.60775685500964	34.410880473790556	19.427748890623864	19.553613780575937
9	26.396063100391704	14.826983948004074	19.771730426951027	39.0052225246532
10	17.81988498741827	26.3778411185918	32.453635047484205	23.348638846505732
11	35.37752568324105	21.79208671189334	21.161715568661428	21.668672036204175
12	21.523276797612777	24.966636645594825	29.89237612263822	23.61771043415418
13	31.17892891857058	19.621529913785764	24.160420999145042	25.03912016849862
14	22.341624572937167	20.903015761776373	25.711144954200023	31.044214711086433
15	25.527497826921625	27.21712276334689	22.704993726975715	24.550385682755774
16	24.34554301267879	25.85259130378518	24.985453339594464	24.816412343941572
17	23.953508730612977	26.207444179123506	25.696800495942394	24.14224659432112
18	24.046045948891862	25.019970435667126	26.480060251482378	24.453923363958634
19	24.950817301081855	24.991162576711925	26.896001963977568	23.162018158228655
20	24.493412254021088	24.94924726087455	26.708334582228954	23.84900590287541
21	24.08279916283553	25.327650739322415	26.951952487728708	23.637597610113342
22	24.60890536320977	25.041118401489733	26.257304849949353	24.092671385351146
23	24.027871544067935	25.20899376123114	26.39242346172932	24.370711232971605
24	24.002156188551364	25.240917912112952	26.493310439292493	24.26361546004319
25	24.416694380255173	24.947034931491533	26.306095038815673	24.330175649437617
26	23.932860323038152	25.413217930620398	26.574286452408515	24.079635293932935
27	24.27981542036399	25.558399072819892	26.33311876117167	23.828666745644448
28	24.05396751539234	25.604881778351242	26.228592144946113	24.11255856131031
29	23.94368408507334	25.797330646185728	26.623576199522613	23.635409069218316
30	24.151428950685037	25.60195579432854	26.406244573251175	23.840370681735244
31	24.07157099650452	25.11512438762485	26.67003511656597	24.14326949930466
32	23.58126647055437	25.663115996949365	26.521666316975896	24.23395121552037
33	23.691787785753267	25.236754926714806	26.52763722746124	24.54382006007069
34	24.133516219228994	25.164390346250958	26.70502798239842	23.997065452121625
35	23.757087185284252	25.295583857512664	26.727341584132507	24.219987373070577
36	23.617543914738253	24.925768023228983	27.047035074222464	24.409652987810304
37	23.394098647053536	24.607763515786278	27.405242126367302	24.592895710792885
38	23.285527987869774	24.566205027268744	27.751364626613512	24.396902358247967
39	22.924371163214243	24.4701946897434	28.22047360976508	24.384960537277276
40	23.22598540243223	24.372875985378645	28.213907987079995	24.18723062510913
41	22.690554114766133	24.471431691118852	28.350572850579276	24.48744134353574
42	22.331205215197798	24.769406291674457	28.22323307437185	24.67615541875589
43	22.253345494008396	24.473786751429806	28.16992307278754	25.102944681774257
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1968.0
1	1711.0
2	1454.0
3	2877.5
4	4301.0
5	4301.0
6	5568.0
7	6835.0
8	6609.5
9	6384.0
10	9351.5
11	12319.0
12	12319.0
13	20094.5
14	27870.0
15	35421.5
16	42973.0
17	38743.0
18	34513.0
19	34513.0
20	38931.0
21	43349.0
22	35639.5
23	27930.0
24	32463.0
25	36996.0
26	36996.0
27	44358.5
28	51721.0
29	58852.5
30	65984.0
31	75958.0
32	85932.0
33	85932.0
34	99020.0
35	112108.0
36	129139.5
37	146171.0
38	165571.0
39	184971.0
40	184971.0
41	205917.0
42	226863.0
43	249775.5
44	272688.0
45	294337.0
46	315986.0
47	315986.0
48	336681.0
49	357376.0
50	365662.5
51	373949.0
52	369896.5
53	365844.0
54	365844.0
55	349011.0
56	332178.0
57	310631.5
58	289085.0
59	263902.5
60	238720.0
61	238720.0
62	209757.5
63	180795.0
64	153862.0
65	126929.0
66	105703.5
67	84478.0
68	84478.0
69	69555.0
70	54632.0
71	44886.5
72	35141.0
73	28959.0
74	22777.0
75	22777.0
76	18574.5
77	14372.0
78	11520.0
79	8668.0
80	6745.5
81	4823.0
82	4823.0
83	3687.0
84	2551.0
85	1909.5
86	1268.0
87	915.0
88	562.0
89	562.0
90	370.5
91	179.0
92	113.5
93	48.0
94	33.5
95	19.0
96	19.0
97	11.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4203714.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.50467071552633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.32498609047431	44.58411903181009
2	10.07247246511579	11.181385349349153
3	3.3442971423674877	5.568723349859492
4	1.7086692594110653	3.7935649842141372
5	1.018774077921752	2.8273359864280403
6	0.681058905379703	2.2681170168586333
7	0.47444804775564203	1.8433857863610854
8	0.34953468314467245	1.55206459932807
9	0.2764476693901143	1.3809723173615682
>10	1.621212892288359	16.514617185069934
>50	0.09606191315690772	3.611691196748286
>100	0.030879425988316812	2.800675564801585
>500	6.430154065712082E-4	0.2400295441277015
>1k	3.429414661095039E-4	0.37498660707679643
>5k	4.286768326368121E-5	0.21671180312532856
>10k+	1.2860304979104363E-4	1.2416196774801298
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20266	0.48209749759379444	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18563	0.44158570254779467	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13354	0.3176714686108522	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9108	0.21666554860773116	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.3788487989430298E-5	4.7576975978860595E-5
2	0.0	0.0	0.0	2.3788487989430298E-5	7.13654639682909E-5
3	0.0	0.0	0.0	2.3788487989430298E-5	7.13654639682909E-5
4	0.0	0.0	0.0	2.3788487989430298E-5	7.13654639682909E-5
5	0.0	0.0	0.0	2.3788487989430298E-5	7.13654639682909E-5
6	2.3788487989430298E-5	0.0	0.0	2.3788487989430298E-5	7.13654639682909E-5
7	2.3788487989430298E-5	0.0	0.0	2.3788487989430298E-5	7.13654639682909E-5
8	4.7576975978860595E-5	0.0	0.0	2.3788487989430298E-5	7.13654639682909E-5
9	1.1894243994715149E-4	0.0	0.0	4.7576975978860595E-5	1.9030790391544238E-4
10	1.427309279365818E-4	0.0	0.0	4.7576975978860595E-5	1.9030790391544238E-4
11	1.427309279365818E-4	0.0	0.0	4.7576975978860595E-5	1.9030790391544238E-4
12	1.665194159260121E-4	0.0	0.0	4.7576975978860595E-5	1.9030790391544238E-4
13	1.665194159260121E-4	0.0	0.0	7.13654639682909E-5	2.140963919048727E-4
14	2.140963919048727E-4	0.0	0.0	9.515395195772119E-5	3.092503438625939E-4
15	2.140963919048727E-4	0.0	0.0	1.427309279365818E-4	3.8061580783088476E-4
16	2.140963919048727E-4	0.0	0.0	1.427309279365818E-4	4.519812717991757E-4
17	2.140963919048727E-4	0.0	0.0	2.6167336788373327E-4	4.519812717991757E-4
18	2.3788487989430298E-4	0.0	0.0	3.330388318520242E-4	4.995582477780363E-4
19	2.6167336788373327E-4	0.0	0.0	4.519812717991757E-4	4.995582477780363E-4
20	2.854618558731636E-4	0.0	0.0	5.471352237568968E-4	4.995582477780363E-4
21	2.854618558731636E-4	0.0	0.0	9.515395195772119E-4	5.709237117463272E-4
22	2.854618558731636E-4	0.0	0.0	0.0016176171832812604	5.709237117463272E-4
23	2.854618558731636E-4	0.0	0.0	0.002140963919048727	7.850201036511999E-4
24	2.854618558731636E-4	0.0	0.0	0.0033065998305308116	8.088085916406302E-4
25	2.854618558731636E-4	0.0	0.0	0.004448447254023466	8.088085916406302E-4
26	2.854618558731636E-4	0.0	0.0	0.007850201036512	8.088085916406302E-4
27	2.854618558731636E-4	0.0	0.0	0.02709508781996111	8.088085916406302E-4
28	2.854618558731636E-4	0.0	0.0	0.07992931964448581	8.088085916406302E-4
29	2.854618558731636E-4	0.0	0.0	0.1659722807022552	8.088085916406302E-4
30	2.854618558731636E-4	0.0	0.0	0.27211651411109317	8.088085916406302E-4
31	2.854618558731636E-4	0.0	0.0	0.5506559199793326	8.325970796300605E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2965	0.0	14.8499155	37
GGTATCA	11360	0.0	14.754401	1
TATACAC	1385	0.0	13.891697	37
ATACCCG	335	0.0	13.80597	5
TCTTATA	4655	0.0	13.790547	37
CGCGTGA	230	7.927156E-9	12.065218	10
TAACCGT	155	2.7237174E-5	11.935484	6
CTCTTAT	6840	0.0	11.819445	37
TAAGACT	505	0.0	11.722773	4
GTATCAA	14450	0.0	11.535295	2
TACCCGT	385	0.0	11.532469	6
TATATCG	145	1.9405462E-4	11.482759	5
TCTCTTA	10005	0.0	11.371814	37
TAATCCG	115	0.005222383	11.26087	5
ACCCGTT	480	0.0	11.177084	7
TCTACAC	705	0.0	11.021276	3
ATTACGG	460	0.0	10.858696	25
TATACCG	205	3.5787143E-6	10.829268	5
CCGTTTA	120	0.0071677077	10.791667	9
AACGCAG	15215	0.0	10.724286	7
>>END_MODULE