FastQCFastQC Report
Thu 9 Feb 2017
SRR2727082.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727082.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3883525
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT215890.5559124764228376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT212970.5483935342247057No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT151350.38972325400248486No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107420.2766043736038779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG3500.015.3285715
TATACAC13750.015.06909137
CTTATAC32550.014.77726637
GGTATCA114550.013.9375821
TCTTATA49250.013.10964537
GTACTTA11600.013.07758630
TACTTAC11400.012.98245631
TAAGGCG2305.7661964E-1012.8695645
CTAGTAC3650.012.6712333
CTCGTTA6500.012.52307722
GGGTAAG18200.012.5027471
TCGTTAC6350.012.2362223
TAGTACT4550.012.1978024
CTCTTAT75400.011.99801137
GTAATAC2757.876224E-1011.4363633
ATACCCG3301.0913936E-1111.2121215
GGAGTGT14700.011.200686
TATACTG5000.011.15
ACCCGAC17400.010.95114911
CTATAGT3053.765308E-1010.9180334
Produced by FastQC (version 0.11.5)