##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727082.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3883525
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.865839411359524	31.0	31.0	34.0	30.0	34.0
2	32.0963318119492	33.0	31.0	34.0	30.0	34.0
3	32.1980126302779	34.0	31.0	34.0	30.0	34.0
4	35.84371955890589	37.0	35.0	37.0	35.0	37.0
5	35.537502655448336	37.0	35.0	37.0	33.0	37.0
6	35.55800851030958	37.0	35.0	37.0	33.0	37.0
7	35.52124139795675	37.0	35.0	37.0	33.0	37.0
8	35.48911774740732	37.0	35.0	37.0	33.0	37.0
9	37.29760385217039	39.0	37.0	39.0	34.0	39.0
10	37.11337354594087	39.0	37.0	39.0	33.0	39.0
11	37.20541106340245	39.0	37.0	39.0	34.0	39.0
12	37.11156333485686	39.0	37.0	39.0	33.0	39.0
13	37.196029123026115	39.0	37.0	39.0	34.0	39.0
14	38.33120373887126	40.0	38.0	41.0	34.0	41.0
15	38.28936855047927	40.0	38.0	41.0	33.0	41.0
16	38.23458018166485	40.0	38.0	41.0	33.0	41.0
17	38.204099368486105	40.0	38.0	41.0	33.0	41.0
18	38.213862148434735	40.0	38.0	41.0	33.0	41.0
19	38.27089435499965	40.0	38.0	41.0	34.0	41.0
20	38.18919692804862	40.0	38.0	41.0	34.0	41.0
21	38.13718258540887	40.0	37.0	41.0	33.0	41.0
22	38.06676820671941	40.0	37.0	41.0	33.0	41.0
23	38.00466972660148	40.0	37.0	41.0	33.0	41.0
24	37.919460541647084	40.0	37.0	41.0	33.0	41.0
25	37.88973291017825	40.0	37.0	41.0	33.0	41.0
26	37.728756220186554	39.0	37.0	41.0	33.0	41.0
27	37.579899447023	39.0	37.0	41.0	32.0	41.0
28	37.48631822892862	39.0	37.0	41.0	32.0	41.0
29	37.38876407387618	39.0	36.0	41.0	32.0	41.0
30	37.31920767859097	39.0	36.0	41.0	32.0	41.0
31	37.246901976941054	39.0	36.0	40.0	31.0	41.0
32	37.122752138842934	39.0	36.0	40.0	31.0	41.0
33	37.034017290992075	39.0	36.0	40.0	31.0	41.0
34	36.98324048383878	39.0	36.0	40.0	31.0	41.0
35	36.888506447106685	39.0	35.0	40.0	31.0	41.0
36	36.75875448207492	39.0	35.0	40.0	30.0	41.0
37	36.654634642496184	39.0	35.0	40.0	30.0	41.0
38	36.59673466760224	39.0	35.0	40.0	30.0	41.0
39	36.474535119511266	39.0	35.0	40.0	30.0	41.0
40	36.38034028363407	39.0	35.0	40.0	30.0	41.0
41	36.30587906605468	39.0	35.0	40.0	30.0	41.0
42	36.145047604946534	39.0	35.0	40.0	29.0	41.0
43	34.8774129173882	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	15.0
11	22.0
12	22.0
13	11.0
14	10.0
15	8.0
16	24.0
17	30.0
18	90.0
19	222.0
20	503.0
21	1156.0
22	2202.0
23	3938.0
24	6795.0
25	11038.0
26	17426.0
27	25811.0
28	37346.0
29	52080.0
30	69950.0
31	92161.0
32	120526.0
33	157002.0
34	208810.0
35	280530.0
36	396697.0
37	595905.0
38	979694.0
39	823497.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.06239820781378	18.47839269735614	13.067252045499899	25.391957049330184
2	18.57904867356332	21.113884937009548	35.63010409357478	24.676962295852352
3	20.39701044798218	22.60734770601451	30.19614911710366	26.799492728899647
4	15.205489857796717	15.738485010396483	33.63488068185475	35.42114444995204
5	15.560759876658448	35.5583393952659	33.53553794555204	15.345362782523608
6	35.14654856090794	33.774496108561166	14.9905305102967	16.088424820234195
7	28.66689412325143	30.481843170830626	21.316638878338622	19.534623827579324
8	26.50777322149336	34.69857925467198	19.595805357246316	19.197842166588345
9	26.469251517628955	14.945262358295622	19.888992603369363	38.69649352070606
10	17.752969274048706	26.61007718503164	32.56070193960384	23.076251601315814
11	35.022228516618284	22.08666095879388	21.206146477748952	21.68496404683889
12	21.329719777779207	25.29820202007197	30.09479789624117	23.27728030590765
13	31.099915669398293	19.8176656516953	24.402005909579568	24.680412769326836
14	22.056816938219786	21.11123270739856	26.179900992011124	30.652049362370526
15	25.32456981736953	27.483870967741936	23.1054003772346	24.086158837653933
16	23.82173412041895	26.10638530716295	25.528611248801024	24.543269323617075
17	23.627838110994524	26.39403634584559	26.012244030874008	23.965881512285875
18	23.664222581288907	25.34305817524028	26.916628578417807	24.076090665053012
19	24.741414050379486	25.2053739836875	27.419934209255764	22.633277756677245
20	24.168249206584225	25.10615999639503	27.20317237561236	23.522418421408386
21	23.653639412647014	25.678629595534986	27.579608731757876	23.088122260060125
22	24.227988747336504	25.239621220411866	26.74140117547846	23.79098885677316
23	23.593281876645573	25.572334412679204	26.985638047907507	23.84874566276772
24	23.50032509125086	25.43907918707875	27.1447718245666	23.91582389710379
25	23.90282024706935	25.21309892430202	26.84712986268918	24.03695096593945
26	23.44043105168629	25.642554122865178	27.268988869648066	23.648025955800463
27	23.91574664769765	25.882284779935755	26.91557283653382	23.28639573583278
28	23.579711730966068	26.053134716526866	26.672958201633822	23.69419535087324
29	23.453202953502295	26.201221828107197	27.18478701694981	23.160788201440702
30	23.845758685730104	25.91076406100128	26.870433433542978	23.373043819725638
31	23.600157073792495	25.45625430504503	27.317372747697	23.626215873465473
32	23.15697723073754	25.991180692798427	27.02011703285031	23.831725043613726
33	23.273907081839308	25.49042429236325	27.0042551547885	24.231413471008942
34	23.570776549655275	25.538730921006042	27.40018411108465	23.490308418254035
35	23.31500376590855	25.495831750793418	27.242286324923874	23.946878158374158
36	23.058097990871694	25.244307684384676	27.632936571800105	24.064657752943525
37	22.80227370752087	24.904951043188856	28.1019949659137	24.190780283376572
38	22.86286299174075	24.76276063627761	28.321872525604956	24.05250384637668
39	22.27249728017716	24.774811543636257	28.971900528514688	23.980790647671895
40	22.680837641060634	24.651212493803953	28.87976773678552	23.788182128349888
41	22.145447756870368	24.77236531244166	28.983539439040563	24.098647491647405
42	21.743519096696946	25.176276707372814	28.861948873768036	24.21825532216221
43	21.600942442754974	24.83102336150791	28.687004718651227	24.881029477085896
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3592.0
1	2985.5
2	2379.0
3	4203.5
4	6028.0
5	6028.0
6	7429.0
7	8830.0
8	8395.0
9	7960.0
10	11445.0
11	14930.0
12	14930.0
13	23577.5
14	32225.0
15	40723.5
16	49222.0
17	43854.5
18	38487.0
19	38487.0
20	42408.0
21	46329.0
22	37661.5
23	28994.0
24	33157.5
25	37321.0
26	37321.0
27	44125.5
28	50930.0
29	57203.0
30	63476.0
31	73207.0
32	82938.0
33	82938.0
34	95130.0
35	107322.0
36	122405.0
37	137488.0
38	154985.0
39	172482.0
40	172482.0
41	191012.0
42	209542.0
43	229339.5
44	249137.0
45	270310.0
46	291483.0
47	291483.0
48	310889.5
49	330296.0
50	338549.0
51	346802.0
52	341816.0
53	336830.0
54	336830.0
55	318972.5
56	301115.0
57	278442.5
58	255770.0
59	233423.5
60	211077.0
61	211077.0
62	184928.5
63	158780.0
64	134025.5
65	109271.0
66	91052.0
67	72833.0
68	72833.0
69	59263.5
70	45694.0
71	37471.0
72	29248.0
73	23964.5
74	18681.0
75	18681.0
76	15161.5
77	11642.0
78	9360.0
79	7078.0
80	5482.0
81	3886.0
82	3886.0
83	2907.5
84	1929.0
85	1407.5
86	886.0
87	651.0
88	416.0
89	416.0
90	280.5
91	145.0
92	90.5
93	36.0
94	23.5
95	11.0
96	11.0
97	6.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3883525.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.2943059468653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.9999164977005	46.40833997489795
2	9.950121081080038	11.401705628555076
3	3.307636449534686	5.685262041019308
4	1.6217632819077554	3.716712065880612
5	0.9359460156087829	2.6812188684019587
6	0.6261831341546381	2.1526036840213703
7	0.42598254230051197	1.7084461874612318
8	0.31353106022494975	1.4370835590698656
9	0.24971801530770002	1.2876678332535025
>10	1.4593225730821828	15.087331601768355
>50	0.08124665419138288	3.120662055133902
>100	0.027238104498326515	2.672073633268686
>500	7.647754041629725E-4	0.3009989073834591
>1k	4.498678602801018E-4	0.567536792565724
>5k	0.0	0.0
>10k+	1.7994714411204062E-4	1.772357167318991
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21589	0.5559124764228376	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21297	0.5483935342247057	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	15135	0.38972325400248486	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10742	0.2766043736038779	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5749802048396753E-5	0.0	0.0	0.0	2.5749802048396753E-5
2	5.1499604096793506E-5	0.0	0.0	2.5749802048396753E-5	2.5749802048396753E-5
3	5.1499604096793506E-5	0.0	0.0	2.5749802048396753E-5	2.5749802048396753E-5
4	5.1499604096793506E-5	0.0	0.0	2.5749802048396753E-5	7.724940614519027E-5
5	5.1499604096793506E-5	0.0	0.0	2.5749802048396753E-5	7.724940614519027E-5
6	5.1499604096793506E-5	0.0	0.0	2.5749802048396753E-5	1.2874901024198377E-4
7	5.1499604096793506E-5	0.0	0.0	2.5749802048396753E-5	1.2874901024198377E-4
8	5.1499604096793506E-5	0.0	0.0	5.1499604096793506E-5	1.5449881229038053E-4
9	7.724940614519027E-5	0.0	0.0	1.0299920819358701E-4	1.5449881229038053E-4
10	1.0299920819358701E-4	0.0	0.0	1.2874901024198377E-4	1.5449881229038053E-4
11	1.5449881229038053E-4	0.0	0.0	1.2874901024198377E-4	1.5449881229038053E-4
12	2.3174821843557078E-4	0.0	0.0	1.2874901024198377E-4	1.5449881229038053E-4
13	2.3174821843557078E-4	2.5749802048396753E-5	0.0	1.5449881229038053E-4	1.5449881229038053E-4
14	3.3474742662915777E-4	5.1499604096793506E-5	0.0	1.5449881229038053E-4	2.3174821843557078E-4
15	3.3474742662915777E-4	5.1499604096793506E-5	0.0	1.8024861433877726E-4	3.0899762458076106E-4
16	3.3474742662915777E-4	5.1499604096793506E-5	0.0	4.1199683277434805E-4	3.862470307259513E-4
17	3.3474742662915777E-4	5.1499604096793506E-5	0.0	5.149960409679351E-4	3.862470307259513E-4
18	3.6049722867755453E-4	5.1499604096793506E-5	0.0	6.179952491615221E-4	4.1199683277434805E-4
19	3.6049722867755453E-4	5.1499604096793506E-5	0.0	7.724940614519026E-4	4.1199683277434805E-4
20	4.1199683277434805E-4	5.1499604096793506E-5	0.0	0.0010557418839842669	4.1199683277434805E-4
21	4.377466348227448E-4	5.1499604096793506E-5	0.0	0.00198273475772655	4.377466348227448E-4
22	4.377466348227448E-4	5.1499604096793506E-5	0.0	0.0030642264437592136	4.377466348227448E-4
23	4.377466348227448E-4	5.1499604096793506E-5	0.0	0.003913969911356306	7.467442594035059E-4
24	4.377466348227448E-4	5.1499604096793506E-5	0.0	0.0057679556588408724	7.467442594035059E-4
25	4.377466348227448E-4	5.1499604096793506E-5	0.0	0.007621941406325439	7.467442594035059E-4
26	4.377466348227448E-4	5.1499604096793506E-5	0.0	0.012256905775036854	7.724940614519026E-4
27	4.377466348227448E-4	5.1499604096793506E-5	0.0	0.03689946633535255	7.982438635002994E-4
28	4.377466348227448E-4	5.1499604096793506E-5	0.0	0.10482744413902319	7.982438635002994E-4
29	4.377466348227448E-4	5.1499604096793506E-5	0.0	0.21925956444209835	7.982438635002994E-4
30	4.892462389195383E-4	5.1499604096793506E-5	0.0	0.3552442690596816	7.982438635002994E-4
31	4.892462389195383E-4	5.1499604096793506E-5	0.0	0.7066003180100553	8.239936655486961E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	350	0.0	15.328571	5
TATACAC	1375	0.0	15.069091	37
CTTATAC	3255	0.0	14.777266	37
GGTATCA	11455	0.0	13.937582	1
TCTTATA	4925	0.0	13.109645	37
GTACTTA	1160	0.0	13.077586	30
TACTTAC	1140	0.0	12.982456	31
TAAGGCG	230	5.7661964E-10	12.869564	5
CTAGTAC	365	0.0	12.671233	3
CTCGTTA	650	0.0	12.523077	22
GGGTAAG	1820	0.0	12.502747	1
TCGTTAC	635	0.0	12.23622	23
TAGTACT	455	0.0	12.197802	4
CTCTTAT	7540	0.0	11.998011	37
GTAATAC	275	7.876224E-10	11.436363	3
ATACCCG	330	1.0913936E-11	11.212121	5
GGAGTGT	1470	0.0	11.20068	6
TATACTG	500	0.0	11.1	5
ACCCGAC	1740	0.0	10.951149	11
CTATAGT	305	3.765308E-10	10.918033	4
>>END_MODULE