FastQCFastQC Report
Thu 9 Feb 2017
SRR2727081.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727081.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2027844
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82570.4071812230132101No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT78320.3862230033473975No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53710.2648625831178335No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35870.17688737397945797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG500.00703669218.49999816
CTTATAC10300.015.44660337
TACATCG1252.9612602E-614.8000015
CGCGTAT750.004105844514.826
TATACCG1001.0940186E-414.7999995
CGGTTAG1651.6470949E-814.57575720
ATACCGA1152.211381E-514.4782616
TCTTATA15250.013.9508237
GGTATCA53850.013.3639741
GTAATAC1857.3910996E-813.03
CTAGTAC1451.373086E-512.758623
TAGCACT3750.012.3333344
GATATAC1050.0026447712.3333333
GTACTAG1359.96732E-512.3333331
CTCTTAT24100.012.12863137
TATACTG2901.2732926E-1112.1206895
TCTCTTA35050.012.03423737
TTACACC3850.012.0129864
TTATACT3600.011.8194454
TTCGCGG2351.0888471E-811.80851116
Produced by FastQC (version 0.11.5)