FastQCFastQC Report
Thu 9 Feb 2017
SRR2727080.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727080.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259188
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36530.29010759314732987No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32820.2606441611578255No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21860.17360394158775339No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16350.12984558302652185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13800.020.2427541
CTTATAC6150.016.8455337
GTATAGG1452.9795046E-916.5862061
GTATCAA18050.015.4764542
TCGTTAC1455.34601E-815.31034523
CTCGTTA1904.5474735E-1014.60526422
TTAGACT1152.2100654E-514.4782614
ACGGGAT800.006299063613.87517
ATAAGGT1601.7834464E-713.8753
TCTTATA10100.013.73762437
ACTAGAC1356.567687E-613.7037033
ATAAGGG950.001245189313.6315793
TATACTG2051.4224497E-913.5365865
GTATACT1102.456652E-413.4545454
ATAGACC1254.845099E-513.323
AGTACCG1551.8877236E-613.1290325
TTAGACC850.00940537913.0588244
TATAGGG850.00940537913.0588242
TAGTCGC850.00940537913.05882433
GGGTAAG5650.012.1150451
Produced by FastQC (version 0.11.5)