##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727080.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1259188
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.175529785862	31.0	30.0	31.0	27.0	34.0
2	30.430799848791445	31.0	30.0	31.0	27.0	34.0
3	30.507214967105785	31.0	30.0	33.0	27.0	34.0
4	34.338229875125876	35.0	35.0	37.0	30.0	37.0
5	33.112504248769845	35.0	32.0	37.0	26.0	37.0
6	33.179047131961234	35.0	32.0	37.0	27.0	37.0
7	33.20302131214719	35.0	32.0	37.0	27.0	37.0
8	33.085257324561546	35.0	32.0	37.0	26.0	37.0
9	34.692579662449134	37.0	33.0	39.0	28.0	39.0
10	34.20838508626194	37.0	32.0	39.0	27.0	39.0
11	34.49027230246794	37.0	33.0	39.0	27.0	39.0
12	34.24698694714372	37.0	32.0	39.0	27.0	39.0
13	34.52314189779445	37.0	33.0	39.0	27.0	39.0
14	35.16975463552702	37.0	33.0	39.0	27.0	40.0
15	35.1780846069054	37.0	33.0	39.0	27.0	40.0
16	35.089647455344235	37.0	33.0	39.0	27.0	40.0
17	34.952724295339536	37.0	33.0	39.0	27.0	40.0
18	34.92662334774474	37.0	33.0	39.0	27.0	40.0
19	34.96708513740601	37.0	33.0	39.0	27.0	40.0
20	34.78219773377764	37.0	33.0	39.0	27.0	40.0
21	34.743533928214056	37.0	33.0	39.0	27.0	40.0
22	34.60503991461164	37.0	32.0	39.0	26.0	40.0
23	34.48752529407841	37.0	32.0	39.0	26.0	40.0
24	34.30547781586228	37.0	32.0	39.0	25.0	40.0
25	34.24840214487432	37.0	32.0	39.0	25.0	40.0
26	33.968896622267685	37.0	32.0	39.0	24.0	40.0
27	33.67005482898503	36.0	31.0	39.0	24.0	40.0
28	33.482564954558015	36.0	31.0	39.0	24.0	40.0
29	33.34170830725833	36.0	31.0	39.0	23.0	40.0
30	33.22087091045976	36.0	31.0	39.0	23.0	40.0
31	33.13648716474427	36.0	30.0	39.0	23.0	40.0
32	32.994996775699896	36.0	30.0	38.0	22.0	40.0
33	32.941009603014	36.0	30.0	38.0	21.0	40.0
34	32.91779861307446	36.0	30.0	38.0	21.0	40.0
35	32.79803412993135	36.0	30.0	38.0	21.0	40.0
36	32.65830757599342	35.0	30.0	38.0	20.0	40.0
37	32.442496275377465	35.0	30.0	38.0	19.0	40.0
38	32.320127733110546	35.0	30.0	38.0	19.0	40.0
39	32.15050175192267	35.0	30.0	38.0	18.0	40.0
40	32.064054771805324	35.0	30.0	38.0	18.0	40.0
41	31.986898699797013	35.0	30.0	38.0	18.0	40.0
42	31.900938541345692	35.0	29.0	38.0	18.0	40.0
43	30.553713186593264	33.0	27.0	38.0	15.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	4.0
13	13.0
14	24.0
15	47.0
16	107.0
17	252.0
18	520.0
19	1137.0
20	2043.0
21	3595.0
22	6134.0
23	9358.0
24	14200.0
25	20332.0
26	28057.0
27	36910.0
28	47839.0
29	59557.0
30	73354.0
31	88121.0
32	103369.0
33	120034.0
34	136344.0
35	147631.0
36	146967.0
37	120853.0
38	73162.0
39	19219.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.27929109870806	20.7855379816199	13.650940129670866	25.28423079000118
2	19.95889414448041	22.274195751547822	33.63905945736459	24.12785064660718
3	21.272121398869746	23.47822565018091	29.53347712970581	25.71617582124353
4	17.075130957410646	16.853956676842536	33.030889747996326	33.04002261775049
5	15.642858731182319	36.0454515131974	33.21179998538741	15.09988977023288
6	35.181402618195214	34.73301842139537	14.801999383729832	15.283579576679573
7	28.909185919814988	30.550799404060395	21.072707173194154	19.46730750293046
8	26.15447415318443	35.407500706804704	19.331902781792714	19.10612235821815
9	26.521139019749235	14.93780118616124	19.432682014123387	39.10837777996613
10	17.09403202698882	27.087456360765827	33.14858464343688	22.669926968808472
11	35.953249236809754	21.88307067729362	21.150455690492603	21.013224395404023
12	21.19056090115217	24.983957915736173	30.07517543051554	23.75030575259612
13	30.79746630368142	20.06404127104134	24.203454924919868	24.935037500357375
14	22.20677134788451	20.924198769365653	25.394301724603473	31.47472815814636
15	25.218156462736303	27.8870986699365	22.307629996473917	24.58711487085328
16	24.33584182822581	26.473806929545074	24.68964126087606	24.500709981353065
17	24.04144575710696	26.45355578356846	25.38373936219214	24.121259097132437
18	23.983789553267663	25.74635399956162	26.160350956330586	24.109505490840128
19	24.911609704031488	25.480388949068768	26.434734130249016	23.173267216650732
20	24.431379587480187	25.310835236676333	26.30846227886543	23.949322896978053
21	24.165335120728596	25.55138708437501	26.70609948633564	23.577178308560757
22	24.729111141465772	25.17304802777663	25.956568836424744	24.141271994332854
23	24.24578379082393	25.412011550300672	26.13049044304742	24.21171421582798
24	24.15580516968078	25.40176685292427	26.093800131513323	24.34862784588163
25	24.64421516088146	25.119124388097724	25.940526752160913	24.2961336988599
26	24.223229573344092	25.458231812882588	26.09626203553401	24.22227657823931
27	24.558604433968558	25.857219096751237	25.60745496303967	23.97672150624053
28	24.270005749737134	25.893274078215484	25.665587664431367	24.17113250761602
29	24.281124025959585	25.91400172174449	26.07084883273983	23.734025419556097
30	24.733320203178558	25.59141287877584	25.711966759530746	23.96330015851485
31	24.539306283096725	25.311867648039847	26.159795042519463	23.989031026343962
32	24.11951193944034	25.69568642649072	25.954662846215182	24.23013878785376
33	24.196148629116543	25.100779232330677	26.019863594634003	24.68320854391878
34	24.388574224023735	25.211564913261565	26.47158327430058	23.928277588414122
35	24.260872879982973	25.26517088790554	26.16416293674971	24.30979329536177
36	24.204963833835773	25.012944850173284	26.441881593534884	24.34020972245606
37	23.995463743301237	24.748409292337602	26.9595961842076	24.296530780153557
38	24.036363116548124	24.65708059479601	26.924653030365604	24.381903258290265
39	23.485929027277898	24.866024771519424	27.48557006578843	24.162476135414252
40	24.09338399031757	24.51500490792479	27.31744584605317	24.074165255704468
41	23.480608137942866	24.677728822066282	27.427278531879274	24.414384508111578
42	23.275714190414774	24.897950107529613	27.293620968433626	24.532714733621987
43	23.246330174684	24.628887822946215	27.078164658494202	25.046617343875578
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	660.0
1	549.0
2	438.0
3	718.0
4	998.0
5	998.0
6	1244.5
7	1491.0
8	1443.5
9	1396.0
10	1905.0
11	2414.0
12	2414.0
13	3984.5
14	5555.0
15	7001.0
16	8447.0
17	7832.5
18	7218.0
19	7218.0
20	8668.5
21	10119.0
22	9402.5
23	8686.0
24	10105.0
25	11524.0
26	11524.0
27	13936.5
28	16349.0
29	18753.5
30	21158.0
31	24538.5
32	27919.0
33	27919.0
34	32578.5
35	37238.0
36	42508.0
37	47778.0
38	53387.0
39	58996.0
40	58996.0
41	65137.0
42	71278.0
43	77978.0
44	84678.0
45	91058.0
46	97438.0
47	97438.0
48	103521.5
49	109605.0
50	111659.0
51	113713.0
52	111733.5
53	109754.0
54	109754.0
55	104488.5
56	99223.0
57	92191.0
58	85159.0
59	77481.5
60	69804.0
61	69804.0
62	60632.5
63	51461.0
64	43828.5
65	36196.0
66	29952.5
67	23709.0
68	23709.0
69	19600.0
70	15491.0
71	12486.5
72	9482.0
73	7698.5
74	5915.0
75	5915.0
76	4738.0
77	3561.0
78	2835.0
79	2109.0
80	1623.0
81	1137.0
82	1137.0
83	859.5
84	582.0
85	457.5
86	333.0
87	230.5
88	128.0
89	128.0
90	80.5
91	33.0
92	23.5
93	14.0
94	7.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1259188.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.803958983205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.7397144240236	66.51017713396806
2	7.070646718069697	10.719660276025769
3	2.1702808598176517	4.935476438389565
4	1.0118166937621547	3.067988446098739
5	0.599998553536993	2.2741132871150276
6	0.35634078554800375	1.620722537503436
7	0.24254162515530048	1.2869930783494596
8	0.18020790239472428	1.0928377953263275
9	0.12085931628839704	0.8245453189197467
>10	0.49568628735714326	5.925647342903794
>50	0.008650496860447563	0.42889252359580454
>100	0.002731132222862744	0.4079254468894708
>500	1.0504099259790865E-4	0.048571390876126753
>1k	4.201639703916346E-4	0.8564489840387203
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3653	0.29010759314732987	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3282	0.2606441611578255	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2186	0.17360394158775339	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1635	0.12984558302652185	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	7.941625873181764E-5
8	0.0	0.0	0.0	0.0	1.5883251746363528E-4
9	0.0	0.0	0.0	0.0	3.9708129365908823E-4
10	0.0	0.0	0.0	0.0	3.9708129365908823E-4
11	0.0	0.0	0.0	0.0	3.9708129365908823E-4
12	0.0	0.0	0.0	0.0	3.9708129365908823E-4
13	0.0	0.0	0.0	0.0	3.9708129365908823E-4
14	0.0	0.0	0.0	0.0	3.9708129365908823E-4
15	0.0	7.941625873181764E-5	0.0	0.0	3.9708129365908823E-4
16	0.0	7.941625873181764E-5	0.0	1.5883251746363528E-4	3.9708129365908823E-4
17	0.0	7.941625873181764E-5	0.0	1.5883251746363528E-4	3.9708129365908823E-4
18	0.0	7.941625873181764E-5	0.0	1.5883251746363528E-4	3.9708129365908823E-4
19	0.0	7.941625873181764E-5	0.0	1.5883251746363528E-4	3.9708129365908823E-4
20	0.0	7.941625873181764E-5	0.0	2.3824877619545295E-4	3.9708129365908823E-4
21	0.0	7.941625873181764E-5	0.0	3.1766503492727056E-4	3.9708129365908823E-4
22	0.0	7.941625873181764E-5	0.0	7.147463285863588E-4	3.9708129365908823E-4
23	0.0	7.941625873181764E-5	0.0	7.941625873181765E-4	7.941625873181765E-4
24	0.0	7.941625873181764E-5	0.0	0.0013500763984409	7.941625873181765E-4
25	0.0	7.941625873181764E-5	0.0	0.0016677414333681707	7.941625873181765E-4
26	0.0	7.941625873181764E-5	0.0	0.002858985314345435	7.941625873181765E-4
27	0.0	7.941625873181764E-5	0.0	0.012309520103431735	7.941625873181765E-4
28	0.0	7.941625873181764E-5	0.0	0.03796097167380884	7.941625873181765E-4
29	0.0	7.941625873181764E-5	0.0	0.08259290908109035	7.941625873181765E-4
30	0.0	7.941625873181764E-5	0.0	0.13659596501872637	7.941625873181765E-4
31	0.0	7.941625873181764E-5	0.0	0.31623554227009787	7.941625873181765E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1380	0.0	20.242754	1
CTTATAC	615	0.0	16.84553	37
GTATAGG	145	2.9795046E-9	16.586206	1
GTATCAA	1805	0.0	15.476454	2
TCGTTAC	145	5.34601E-8	15.310345	23
CTCGTTA	190	4.5474735E-10	14.605264	22
TTAGACT	115	2.2100654E-5	14.478261	4
ACGGGAT	80	0.0062990636	13.875	17
ATAAGGT	160	1.7834464E-7	13.875	3
TCTTATA	1010	0.0	13.737624	37
ACTAGAC	135	6.567687E-6	13.703703	3
ATAAGGG	95	0.0012451893	13.631579	3
TATACTG	205	1.4224497E-9	13.536586	5
GTATACT	110	2.456652E-4	13.454545	4
ATAGACC	125	4.845099E-5	13.32	3
AGTACCG	155	1.8877236E-6	13.129032	5
TTAGACC	85	0.009405379	13.058824	4
TATAGGG	85	0.009405379	13.058824	2
TAGTCGC	85	0.009405379	13.058824	33
GGGTAAG	565	0.0	12.115045	1
>>END_MODULE