FastQCFastQC Report
Thu 9 Feb 2017
SRR2727079.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727079.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919182
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT68880.35890290759292237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT62400.32513852255804815No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42190.2198332414539111No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29750.15501395907214635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31150.016.5698241
CTTATAC10050.016.38308537
TAGTACT2150.016.3488374
CGTATAC800.006300649613.8753
TCTTATA15800.013.58227837
CTAGTAG2051.4242687E-913.5365853
CTCTTAT25100.013.34063737
TATACTA2651.8189894E-1213.2641525
CGCTACG1551.8891933E-613.12903210
GCCTAGT2001.4639227E-812.9499991
GTAAGAG9550.012.785343
GGGTAAG8400.012.773811
GTTACGG3400.012.51470625
GTATCAA41250.012.4678792
TTAGACT2102.8936483E-812.3333344
TAAGGTG2704.3655746E-1112.3333345
ATAACAC1807.468989E-712.3333333
CTATACT2756.184564E-1112.1090914
TGTTACG1705.3131625E-611.97058924
GTCTTAC1705.3131625E-611.9705891
Produced by FastQC (version 0.11.5)