FastQCFastQC Report
Thu 9 Feb 2017
SRR2727078.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727078.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550685
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48180.3107013997040018No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43960.28348761998729594No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29390.1895291435720343No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19680.12691165517174668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21300.018.4131451
CTTATAC8150.015.8895737
ACGACGG750.004105265314.86
GTATCAA27150.014.4456722
TCTTATA12750.014.36470637
TACCCGC800.00629992913.8755
GTATAAA2800.013.2142851
TAGACGC850.00940666613.0588245
CTCTTAT19300.012.8445637
GCGTCAG1755.307338E-712.6857141
TCTATAC1755.307338E-712.6857143
CGTACAC1050.002644285612.3333343
TATACTG2603.092282E-1012.0961545
GCACCGT2002.0406151E-712.0250016
AACGCAG32450.011.9152557
GTTAGGC1257.221193E-411.843
TAGATAG1257.221193E-411.845
GACTGTG5700.011.684217
TATCAAC34600.011.6560693
ATCAACG35550.011.3445844
Produced by FastQC (version 0.11.5)