##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727078.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1550685
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.060501649271128	31.0	31.0	34.0	28.0	34.0
2	31.31669359025205	31.0	31.0	34.0	28.0	34.0
3	31.407023347746318	31.0	31.0	34.0	28.0	34.0
4	35.22978361175868	37.0	35.0	37.0	33.0	37.0
5	34.644834379645125	35.0	35.0	37.0	30.0	37.0
6	34.662581375327676	35.0	35.0	37.0	31.0	37.0
7	34.623606341713504	35.0	35.0	37.0	31.0	37.0
8	34.55995060247568	35.0	35.0	37.0	30.0	37.0
9	36.31952717669933	38.0	35.0	39.0	32.0	39.0
10	36.00546661636631	37.0	35.0	39.0	31.0	39.0
11	36.14737809419708	37.0	35.0	39.0	32.0	39.0
12	35.99331392255681	37.0	35.0	39.0	31.0	39.0
13	36.16778649435572	37.0	35.0	39.0	32.0	39.0
14	37.072719475586595	38.0	36.0	40.0	32.0	41.0
15	37.06951508526877	38.0	36.0	40.0	32.0	41.0
16	37.003637747189146	38.0	36.0	40.0	31.0	41.0
17	36.8954526547945	38.0	36.0	40.0	31.0	41.0
18	36.90570941229198	38.0	36.0	40.0	31.0	41.0
19	36.92599980008835	39.0	36.0	40.0	31.0	41.0
20	36.79195645795245	39.0	36.0	40.0	31.0	41.0
21	36.78475125509049	38.0	36.0	40.0	31.0	41.0
22	36.657132170621374	38.0	36.0	40.0	30.0	41.0
23	36.58349116680693	38.0	35.0	40.0	30.0	41.0
24	36.45198799240336	38.0	35.0	40.0	30.0	41.0
25	36.41479023786262	38.0	35.0	40.0	30.0	41.0
26	36.227362746141225	38.0	35.0	40.0	30.0	41.0
27	35.995802500185405	38.0	34.0	40.0	29.0	41.0
28	35.860620951386004	38.0	34.0	40.0	28.0	41.0
29	35.754686477266496	38.0	34.0	40.0	27.0	41.0
30	35.68273956348323	38.0	34.0	40.0	27.0	41.0
31	35.65031131403219	38.0	34.0	40.0	27.0	41.0
32	35.5292370790973	38.0	34.0	40.0	27.0	41.0
33	35.49702550808191	38.0	34.0	40.0	27.0	41.0
34	35.49108748714278	38.0	34.0	40.0	27.0	41.0
35	35.36688366754047	38.0	34.0	40.0	27.0	41.0
36	35.28192701934951	38.0	34.0	40.0	27.0	41.0
37	35.11235873178627	38.0	33.0	40.0	26.0	41.0
38	35.049072506666406	38.0	33.0	40.0	26.0	41.0
39	34.9361501529969	38.0	33.0	40.0	26.0	41.0
40	34.856732347317475	38.0	33.0	40.0	25.0	41.0
41	34.7966956538562	38.0	33.0	40.0	25.0	41.0
42	34.73722774128853	38.0	33.0	40.0	25.0	41.0
43	33.37644589326652	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	0.0
12	1.0
13	0.0
14	4.0
15	3.0
16	18.0
17	45.0
18	115.0
19	237.0
20	498.0
21	1049.0
22	1984.0
23	3507.0
24	5755.0
25	9092.0
26	13772.0
27	19858.0
28	27975.0
29	37328.0
30	49524.0
31	63001.0
32	78976.0
33	98990.0
34	124681.0
35	158378.0
36	206762.0
37	267006.0
38	293358.0
39	88763.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.56221282852417	19.485130764791045	13.141224684574881	25.811431722109905
2	19.004375485672462	21.202565317907894	34.83221930953095	24.960839886888696
3	20.42033037012675	22.58892038034804	29.885631188797205	27.105118060728
4	15.758777572492159	16.122745754295682	33.54691636276871	34.57156031044345
5	15.670171569338711	35.48148076495226	33.608631024353755	15.239716641355272
6	35.503728997185114	34.15245520528025	14.962290858556058	15.38152493897858
7	29.293312310366066	30.040465987611924	21.07062362762263	19.59559807439938
8	26.2206057323054	34.869686622363666	19.371697024218328	19.538010621112605
9	26.163276229537267	14.908056762011626	19.78918993864002	39.139477069811086
10	17.40675894846471	26.594762959595275	33.07267433424583	22.92580375769418
11	35.73117686699749	21.640823249080245	21.363913367318315	21.264086516603953
12	21.39267485014687	24.787497138361434	30.162154144781177	23.657673866710518
13	30.768015425441014	19.873475270606217	24.22490705720375	25.133602246749014
14	22.22275961913606	20.790940777785302	25.43172855866923	31.554571044409407
15	25.149595178904804	27.545955497086773	22.491737522449757	24.812711801558667
16	24.560887607734646	26.18668523910401	24.515810754602	24.736616398559345
17	24.021706536143704	26.348936115329675	25.559156114878263	24.070201233648355
18	24.132947697307962	25.372206476492646	26.205451139335196	24.289394686864192
19	24.88493794677836	25.29359605593657	26.54227002905168	23.27919596823339
20	24.54418531165259	25.220209133382987	26.382147244604802	23.853458310359617
21	24.233999812985875	25.392197641687382	26.478685226206483	23.89511731912026
22	24.685413220608957	25.093555428729886	26.05783895504245	24.16319239561871
23	24.229679141798623	25.239619909910783	26.000058038866698	24.5306429094239
24	24.30087348494375	25.29243527860268	26.00695821524036	24.399733021213205
25	24.62769679206286	25.116126099111035	25.873081896065287	24.38309521276081
26	24.25708638440431	25.377365486865482	26.14180184886034	24.223746279869864
27	24.48795209858869	25.566443217029892	25.82058896552169	24.12501571885973
28	24.339759525628995	25.514272724634594	25.926929066831754	24.21903868290465
29	24.257860235960237	25.743526248077465	26.06609337163899	23.932520144323316
30	24.268113769076248	25.66704391930018	26.002637544053115	24.06220476757046
31	24.424754221521457	25.068985641829254	26.21054566207837	24.295714474570914
32	23.939871734104607	25.512596046263425	26.117296549589376	24.430235670042595
33	24.091998052473585	25.19680012381625	26.084730296610854	24.626471527099312
34	24.321702989324073	25.114126982591568	26.3376507801391	24.226519247945262
35	24.159194162579762	25.2335580727227	26.299216152861476	24.30803161183606
36	24.040601411634213	24.916859323460276	26.51518522459429	24.527354040311216
37	23.95115706929518	24.54966676017373	26.840783266749856	24.65839290378123
38	23.582674753415425	24.62756781680354	27.29271257541022	24.497044854370813
39	23.39559613977049	24.502719765780927	27.571557086061965	24.530127008386614
40	23.52605461457356	24.453967117757635	27.732453722064765	24.28752454560404
41	23.21451487568397	24.427462701967194	27.736838880881677	24.621183541467158
42	22.907166832722314	24.716496258105288	27.6202452464556	24.756091662716802
43	22.98094068105386	24.41488761418341	27.57213747472891	25.032034230033823
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	750.0
1	600.5
2	451.0
3	827.0
4	1203.0
5	1203.0
6	1504.0
7	1805.0
8	1780.0
9	1755.0
10	2332.0
11	2909.0
12	2909.0
13	4757.0
14	6605.0
15	8637.0
16	10669.0
17	9922.0
18	9175.0
19	9175.0
20	10873.0
21	12571.0
22	11460.0
23	10349.0
24	12198.5
25	14048.0
26	14048.0
27	16959.0
28	19870.0
29	22626.0
30	25382.0
31	29738.5
32	34095.0
33	34095.0
34	39379.0
35	44663.0
36	51250.0
37	57837.0
38	65040.5
39	72244.0
40	72244.0
41	80301.0
42	88358.0
43	96207.0
44	104056.0
45	111622.5
46	119189.0
47	119189.0
48	125849.0
49	132509.0
50	134748.0
51	136987.0
52	135424.0
53	133861.0
54	133861.0
55	128281.5
56	122702.0
57	114618.5
58	106535.0
59	97249.0
60	87963.0
61	87963.0
62	76954.0
63	65945.0
64	56125.0
65	46305.0
66	38334.0
67	30363.0
68	30363.0
69	24899.5
70	19436.0
71	15743.5
72	12051.0
73	9796.5
74	7542.0
75	7542.0
76	6118.0
77	4694.0
78	3788.5
79	2883.0
80	2188.0
81	1493.0
82	1493.0
83	1156.0
84	819.0
85	600.5
86	382.0
87	279.5
88	177.0
89	177.0
90	108.0
91	39.0
92	25.5
93	12.0
94	7.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1550685.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.6237217310932
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.38894808591778	60.304853085306185
2	8.092380829400193	11.430281036751847
3	2.5848590079983564	5.476570898849565
4	1.2517153209637775	3.5360317805716726
5	0.7120438575138524	2.514359362669624
6	0.4533993802881294	1.9212450999911372
7	0.32801903566930246	1.6216147568327244
8	0.23482882725034365	1.326758860013378
9	0.16874247379913615	1.0725499362425828
>10	0.7661239206944109	8.738575188492623
>50	0.016012697289606664	0.7295647955402461
>100	0.0021035872855078184	0.22754661316049782
>500	4.572088498229955E-4	0.1881203199359312
>1k	3.6576707985839645E-4	0.9119282656420477
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4818	0.3107013997040018	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4396	0.28348761998729594	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2939	0.1895291435720343	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1968	0.12691165517174668	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.448762966044039E-5
2	0.0	0.0	0.0	0.0	6.448762966044039E-5
3	0.0	0.0	0.0	0.0	6.448762966044039E-5
4	0.0	0.0	0.0	0.0	1.2897525932088077E-4
5	0.0	0.0	0.0	0.0	1.2897525932088077E-4
6	0.0	0.0	0.0	0.0	2.5795051864176155E-4
7	0.0	0.0	0.0	0.0	2.5795051864176155E-4
8	0.0	0.0	0.0	0.0	3.2243814830220193E-4
9	0.0	0.0	0.0	0.0	4.514134076230827E-4
10	0.0	0.0	0.0	6.448762966044039E-5	4.514134076230827E-4
11	0.0	0.0	0.0	1.2897525932088077E-4	4.514134076230827E-4
12	0.0	0.0	0.0	1.2897525932088077E-4	4.514134076230827E-4
13	0.0	0.0	0.0	1.2897525932088077E-4	4.514134076230827E-4
14	0.0	0.0	0.0	1.2897525932088077E-4	4.514134076230827E-4
15	0.0	0.0	0.0	1.9346288898132116E-4	4.514134076230827E-4
16	0.0	0.0	0.0	1.9346288898132116E-4	5.159010372835231E-4
17	0.0	0.0	0.0	1.9346288898132116E-4	5.159010372835231E-4
18	0.0	0.0	0.0	2.5795051864176155E-4	5.159010372835231E-4
19	0.0	0.0	0.0	2.5795051864176155E-4	5.159010372835231E-4
20	0.0	0.0	0.0	3.2243814830220193E-4	5.159010372835231E-4
21	0.0	0.0	0.0	4.514134076230827E-4	5.803886669439634E-4
22	0.0	0.0	0.0	5.159010372835231E-4	5.803886669439634E-4
23	0.0	0.0	0.0	9.028268152461654E-4	7.093639262648442E-4
24	0.0	0.0	0.0	0.0014187278525296884	7.093639262648442E-4
25	0.0	0.0	0.0	0.001999116519473652	7.093639262648442E-4
26	0.0	0.0	0.0	0.0038047701499659826	7.093639262648442E-4
27	0.0	0.0	0.0	0.015670494007487015	7.093639262648442E-4
28	0.0	0.0	0.0	0.053266782099523756	7.093639262648442E-4
29	0.0	0.0	0.0	0.1050503487168574	7.093639262648442E-4
30	0.0	0.0	0.0	0.17153709489677144	7.093639262648442E-4
31	0.0	0.0	0.0	0.37899379951440815	7.093639262648442E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2130	0.0	18.413145	1
CTTATAC	815	0.0	15.88957	37
ACGACGG	75	0.0041052653	14.8	6
GTATCAA	2715	0.0	14.445672	2
TCTTATA	1275	0.0	14.364706	37
TACCCGC	80	0.006299929	13.875	5
GTATAAA	280	0.0	13.214285	1
TAGACGC	85	0.009406666	13.058824	5
CTCTTAT	1930	0.0	12.84456	37
GCGTCAG	175	5.307338E-7	12.685714	1
TCTATAC	175	5.307338E-7	12.685714	3
CGTACAC	105	0.0026442856	12.333334	3
TATACTG	260	3.092282E-10	12.096154	5
GCACCGT	200	2.0406151E-7	12.025001	6
AACGCAG	3245	0.0	11.915255	7
GTTAGGC	125	7.221193E-4	11.84	3
TAGATAG	125	7.221193E-4	11.84	5
GACTGTG	570	0.0	11.68421	7
TATCAAC	3460	0.0	11.656069	3
ATCAACG	3555	0.0	11.344584	4
>>END_MODULE