FastQCFastQC Report
Thu 9 Feb 2017
SRR2727077.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727077.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3145010
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT77650.2468990559648459No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67660.21513445108282647No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50100.1592999704293468No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36110.11481680503400626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29000.019.0103441
CTTATAC15450.015.80582637
TCTTATA23700.013.89451437
GTATCAA41100.013.2785882
CTCTTAT36400.012.35027437
TTATACT5600.012.2232154
TAGACTA2002.043198E-712.0250015
AAGCGTA1257.224826E-411.845
GTACTAG2052.8154318E-711.7317071
TAGACAG6800.011.4264715
TCTCTTA53100.011.32297637
GTAGCAC3600.011.3055543
TAGCACA4800.011.1770834
TTAGACT3155.638867E-1111.15873054
GTACTAC3700.011.01
TATACTT5600.010.9017865
GGTATAT3104.984031E-1010.7419351
ACCGACC2952.5138434E-910.6610168
AACGCAG50700.010.6548337
CGTGCGA1400.001863452810.57142910
Produced by FastQC (version 0.11.5)