##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727077.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3145010
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.127623759542896	31.0	31.0	34.0	28.0	34.0
2	31.383651562316178	31.0	31.0	34.0	28.0	34.0
3	31.47986174924722	31.0	31.0	34.0	28.0	34.0
4	35.28566077691327	37.0	35.0	37.0	33.0	37.0
5	34.730950299045155	35.0	35.0	37.0	32.0	37.0
6	34.74756582649976	35.0	35.0	37.0	32.0	37.0
7	34.70635419283246	35.0	35.0	37.0	32.0	37.0
8	34.637141058375015	35.0	35.0	37.0	31.0	37.0
9	36.41585813717604	38.0	35.0	39.0	32.0	39.0
10	36.11219614564023	37.0	35.0	39.0	32.0	39.0
11	36.25308154823037	38.0	35.0	39.0	32.0	39.0
12	36.100457232250456	37.0	35.0	39.0	32.0	39.0
13	36.26942171884986	38.0	35.0	39.0	32.0	39.0
14	37.19953895218139	39.0	36.0	40.0	32.0	41.0
15	37.193730067630945	39.0	36.0	40.0	32.0	41.0
16	37.130611031443465	39.0	36.0	40.0	32.0	41.0
17	37.014879761908546	38.0	36.0	40.0	31.0	41.0
18	37.02355159443054	38.0	36.0	40.0	31.0	41.0
19	37.059234787806716	39.0	36.0	40.0	31.0	41.0
20	36.91812585651556	39.0	36.0	40.0	31.0	41.0
21	36.91176530440285	39.0	36.0	40.0	31.0	41.0
22	36.794156457372154	39.0	36.0	40.0	31.0	41.0
23	36.721492459483436	38.0	36.0	40.0	31.0	41.0
24	36.595077281153316	38.0	35.0	40.0	30.0	41.0
25	36.56474128858096	38.0	35.0	40.0	30.0	41.0
26	36.38664487553299	38.0	35.0	40.0	30.0	41.0
27	36.16692029596091	38.0	35.0	40.0	29.0	41.0
28	36.04252895857247	38.0	35.0	40.0	29.0	41.0
29	35.94721447626558	38.0	34.0	40.0	29.0	41.0
30	35.88499623212645	38.0	34.0	40.0	29.0	41.0
31	35.84918617110915	38.0	34.0	40.0	29.0	41.0
32	35.743716236196384	38.0	34.0	40.0	28.0	41.0
33	35.71926893714169	38.0	34.0	40.0	28.0	41.0
34	35.7151735606564	38.0	34.0	40.0	28.0	41.0
35	35.59846359788999	38.0	34.0	40.0	27.0	41.0
36	35.51084066505353	38.0	34.0	40.0	27.0	41.0
37	35.35069395645801	38.0	34.0	40.0	27.0	41.0
38	35.297144365200744	38.0	34.0	40.0	27.0	41.0
39	35.19018635870792	38.0	33.0	40.0	26.0	41.0
40	35.115157980419774	38.0	33.0	40.0	26.0	41.0
41	35.06154288857587	38.0	33.0	40.0	26.0	41.0
42	35.005433687015305	38.0	33.0	40.0	26.0	41.0
43	33.632019929984324	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	3.0
12	2.0
13	2.0
14	4.0
15	12.0
16	26.0
17	77.0
18	175.0
19	370.0
20	847.0
21	1794.0
22	3474.0
23	6150.0
24	10553.0
25	16788.0
26	25474.0
27	36889.0
28	52156.0
29	71544.0
30	93790.0
31	121609.0
32	152930.0
33	193781.0
34	244842.0
35	314849.0
36	412505.0
37	543520.0
38	630009.0
39	210832.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.472046193811785	19.37453299035615	12.952836397976478	26.200584417855588
2	18.872149850079968	20.94915437470787	35.0448488240101	25.133846951202067
3	20.191032778910085	22.67550818598351	29.68111389152976	27.45234514357665
4	15.562398847698416	15.974257633521038	33.79388300832112	34.66946051045943
5	15.63883739638348	35.37979211512841	33.74685613082311	15.234514357664999
6	35.484465868153045	34.371719008842575	15.05419696598739	15.08961815701699
7	29.387505922079736	29.879936788754247	21.267213776744747	19.465343512421267
8	26.252348959144804	34.870540952175034	19.476663031278118	19.400447057402044
9	26.246339439302258	14.864722210740188	19.61866575940935	39.2702725905482
10	17.18789447410342	26.43737221821234	33.26024400558345	23.11448930210079
11	35.85203226698802	21.63182311026038	21.421489915771332	21.094654706980265
12	21.32085430570968	24.705772000724956	30.280825816134133	23.692547877431235
13	30.72588004489652	19.791479200384103	24.239096219089923	25.243544535629457
14	22.275477661438277	20.515101700789504	25.41022127115653	31.79919936661569
15	25.05642271407722	27.609387569514883	22.30736945192543	25.026820264482463
16	24.67756859278667	26.072985459505695	24.425391334208793	24.824054613498845
17	24.06663253852929	26.304177093236586	25.422494682051884	24.20669568618224
18	24.263356873269085	25.439346774732037	25.965481826766844	24.331814525232033
19	24.954801415575787	25.218584360622064	26.270727279086554	23.5558869447156
20	24.48618605346247	25.201954842750894	26.168819812973567	24.143039290813064
21	24.305264530160475	25.250126390695097	26.313938588430563	24.13067049071386
22	24.72656684716424	25.052130199903978	25.88688112279452	24.334421830137266
23	24.388730083529143	25.08675648090149	25.887453458017625	24.63705997755174
24	24.32377003570736	25.28160482796557	25.822016464176585	24.572608672150487
25	24.689524039669188	25.010222543012585	25.79530748709861	24.50494593021962
26	24.40580475101828	25.217566875781	25.94732608163408	24.42930229156664
27	24.505772636652985	25.56993459480256	25.64853529877488	24.27575746976957
28	24.464882464602656	25.349935294323387	25.844083166667197	24.341099074406756
29	24.310542732773506	25.621095004467392	25.942938178256984	24.125424084502118
30	24.449524802782822	25.490634370002002	25.75727899116378	24.302561836051396
31	24.472640786515782	24.94001608897905	26.111204733848226	24.476138390656946
32	24.00297614316012	25.433464440494625	26.017309960858633	24.546249455486628
33	24.143325458424616	25.1166450981078	26.010664512990424	24.729364930477168
34	24.4882210231446	24.874070352717478	26.296641346132443	24.341067278005475
35	24.200972333951242	25.129045694608283	26.218835552192203	24.45114641924827
36	24.124915342081582	24.859030654910477	26.371076721536657	24.644977281471284
37	24.020209792655667	24.482593060117456	26.726433302278846	24.77076384494803
38	23.75521858436062	24.557378195935783	26.999818760512685	24.68758445919091
39	23.567397241980153	24.45597947224333	27.313681037580167	24.662942248196348
40	23.635409744325138	24.34930254593785	27.56703476300552	24.44825294673149
41	23.40157900928773	24.352704760875163	27.59571511696306	24.650001112874044
42	23.096587928178288	24.647425604370095	27.435238679686236	24.82074778776538
43	23.069878951100314	24.33175093242947	27.47854537823409	25.119824738236122
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1071.0
1	937.0
2	803.0
3	1478.5
4	2154.0
5	2154.0
6	2658.0
7	3162.0
8	2983.0
9	2804.0
10	3793.0
11	4782.0
12	4782.0
13	8032.0
14	11282.0
15	13993.0
16	16704.0
17	16194.5
18	15685.0
19	15685.0
20	18911.5
21	22138.0
22	21100.5
23	20063.0
24	24115.0
25	28167.0
26	28167.0
27	34404.5
28	40642.0
29	47196.0
30	53750.0
31	63130.0
32	72510.0
33	72510.0
34	83743.5
35	94977.0
36	107756.0
37	120535.0
38	135038.5
39	149542.0
40	149542.0
41	165134.0
42	180726.0
43	195569.5
44	210413.0
45	224499.5
46	238586.0
47	238586.0
48	251448.5
49	264311.0
50	270144.5
51	275978.0
52	273237.0
53	270496.0
54	270496.0
55	259751.0
56	249006.0
57	233382.5
58	217759.0
59	198564.5
60	179370.0
61	179370.0
62	157354.0
63	135338.0
64	115490.0
65	95642.0
66	79300.5
67	62959.0
68	62959.0
69	51597.5
70	40236.0
71	32719.5
72	25203.0
73	20544.0
74	15885.0
75	15885.0
76	12902.0
77	9919.0
78	7926.0
79	5933.0
80	4643.0
81	3353.0
82	3353.0
83	2557.5
84	1762.0
85	1333.0
86	904.0
87	617.5
88	331.0
89	331.0
90	210.0
91	89.0
92	59.5
93	30.0
94	20.0
95	10.0
96	10.0
97	5.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3145010.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.71129850309614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.30316706159488	53.07352942914556
2	8.925700282478553	11.373359100923212
3	2.9198542475917253	5.580831166615487
4	1.3539667200833223	3.4505191146594623
5	0.8163922653017117	2.60067056551281
6	0.5393190175764377	2.0616428950325383
7	0.40144051531010494	1.7903407551511181
8	0.27391461838131403	1.396116481284285
9	0.22187578580079603	1.2722394977787181
>10	1.1800839602757454	13.233712436484272
>50	0.05241472247965771	2.211681922622341
>100	0.01137164413449062	1.0280083464854393
>500	1.4974769741432008E-4	0.07956877288586194
>1k	1.996635965509394E-4	0.2263353143985436
>5k	1.4974769741320454E-4	0.6214442010203655
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7765	0.2468990559648459	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6766	0.21513445108282647	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5010	0.1592999704293468	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3611	0.11481680503400626	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.1796401283302755E-5
2	0.0	0.0	0.0	0.0	3.1796401283302755E-5
3	0.0	0.0	0.0	0.0	3.1796401283302755E-5
4	0.0	0.0	0.0	0.0	6.359280256660551E-5
5	0.0	0.0	0.0	0.0	6.359280256660551E-5
6	0.0	0.0	0.0	0.0	6.359280256660551E-5
7	0.0	0.0	0.0	0.0	6.359280256660551E-5
8	0.0	0.0	0.0	0.0	9.538920384990827E-5
9	0.0	0.0	0.0	0.0	1.9077840769981654E-4
10	0.0	0.0	0.0	0.0	1.9077840769981654E-4
11	0.0	0.0	0.0	0.0	1.9077840769981654E-4
12	0.0	0.0	0.0	0.0	1.9077840769981654E-4
13	0.0	0.0	0.0	0.0	2.2257480898311928E-4
14	0.0	0.0	0.0	3.1796401283302755E-5	2.861676115497248E-4
15	0.0	0.0	0.0	3.1796401283302755E-5	3.1796401283302756E-4
16	0.0	0.0	0.0	6.359280256660551E-5	3.497604141163303E-4
17	0.0	0.0	0.0	9.538920384990827E-5	3.497604141163303E-4
18	0.0	0.0	0.0	1.2718560513321102E-4	4.1335321668293585E-4
19	0.0	0.0	0.0	1.5898200641651378E-4	4.1335321668293585E-4
20	0.0	0.0	0.0	1.9077840769981654E-4	4.1335321668293585E-4
21	0.0	0.0	0.0	3.1796401283302756E-4	4.1335321668293585E-4
22	0.0	0.0	0.0	5.087424205328441E-4	4.1335321668293585E-4
23	0.0	3.1796401283302755E-5	0.0	6.677244269493579E-4	6.677244269493579E-4
24	0.0	3.1796401283302755E-5	0.0	8.585028346491745E-4	6.677244269493579E-4
25	0.0	3.1796401283302755E-5	0.0	0.001558023662881835	6.677244269493579E-4
26	0.0	3.1796401283302755E-5	0.0	0.0032432329308968813	6.995208282326606E-4
27	0.0	3.1796401283302755E-5	0.0	0.012019039685088441	6.995208282326606E-4
28	0.0	3.1796401283302755E-5	0.0	0.041939453292676336	6.995208282326606E-4
29	3.1796401283302755E-5	3.1796401283302755E-5	0.0	0.0841968705981857	6.995208282326606E-4
30	3.1796401283302755E-5	3.1796401283302755E-5	0.0	0.14006314765294864	6.995208282326606E-4
31	3.1796401283302755E-5	3.1796401283302755E-5	0.0	0.3344027522964951	7.313172295159634E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2900	0.0	19.010344	1
CTTATAC	1545	0.0	15.805826	37
TCTTATA	2370	0.0	13.894514	37
GTATCAA	4110	0.0	13.278588	2
CTCTTAT	3640	0.0	12.350274	37
TTATACT	560	0.0	12.223215	4
TAGACTA	200	2.043198E-7	12.025001	5
AAGCGTA	125	7.224826E-4	11.84	5
GTACTAG	205	2.8154318E-7	11.731707	1
TAGACAG	680	0.0	11.426471	5
TCTCTTA	5310	0.0	11.322976	37
GTAGCAC	360	0.0	11.305554	3
TAGCACA	480	0.0	11.177083	4
TTAGACT	315	5.638867E-11	11.1587305	4
GTACTAC	370	0.0	11.0	1
TATACTT	560	0.0	10.901786	5
GGTATAT	310	4.984031E-10	10.741935	1
ACCGACC	295	2.5138434E-9	10.661016	8
AACGCAG	5070	0.0	10.654833	7
CGTGCGA	140	0.0018634528	10.571429	10
>>END_MODULE