##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727076.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8111401
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07445939363619	33.0	31.0	34.0	30.0	34.0
2	32.29760777453858	34.0	31.0	34.0	30.0	34.0
3	32.40627938379572	34.0	31.0	34.0	30.0	34.0
4	35.99998532929145	37.0	35.0	37.0	35.0	37.0
5	35.717965244228466	37.0	35.0	37.0	33.0	37.0
6	35.73060239531987	37.0	35.0	37.0	33.0	37.0
7	35.69800851912019	37.0	35.0	37.0	33.0	37.0
8	35.66605238725098	37.0	35.0	37.0	33.0	37.0
9	37.487175026854175	39.0	37.0	39.0	35.0	39.0
10	37.31913648456044	39.0	37.0	39.0	34.0	39.0
11	37.40452456979997	39.0	37.0	39.0	34.0	39.0
12	37.31208677761092	39.0	37.0	39.0	34.0	39.0
13	37.37823268754683	39.0	37.0	39.0	34.0	39.0
14	38.598976305079724	40.0	38.0	41.0	34.0	41.0
15	38.54671246064644	40.0	38.0	41.0	34.0	41.0
16	38.50698430517737	40.0	38.0	41.0	34.0	41.0
17	38.46197925611124	40.0	38.0	41.0	34.0	41.0
18	38.45749112884445	40.0	38.0	41.0	34.0	41.0
19	38.50352016871068	40.0	38.0	41.0	34.0	41.0
20	38.42525834932831	40.0	38.0	41.0	34.0	41.0
21	38.367272435427616	40.0	38.0	41.0	34.0	41.0
22	38.31025897992221	40.0	38.0	41.0	34.0	41.0
23	38.25432462283643	40.0	38.0	41.0	34.0	41.0
24	38.1809940107757	40.0	38.0	41.0	33.0	41.0
25	38.14887280261449	40.0	38.0	41.0	33.0	41.0
26	37.99611854968087	40.0	37.0	41.0	33.0	41.0
27	37.87811673963598	40.0	37.0	41.0	33.0	41.0
28	37.80671538739116	40.0	37.0	41.0	33.0	41.0
29	37.71889233438219	40.0	37.0	41.0	33.0	41.0
30	37.668946461899736	40.0	37.0	41.0	33.0	41.0
31	37.62991584314473	40.0	37.0	41.0	33.0	41.0
32	37.53022739228402	40.0	37.0	41.0	32.0	41.0
33	37.44998243829888	39.0	37.0	41.0	32.0	41.0
34	37.41369215996102	39.0	37.0	41.0	32.0	41.0
35	37.34781155561166	39.0	36.0	41.0	32.0	41.0
36	37.23700566646872	39.0	36.0	41.0	31.0	41.0
37	37.16144929833946	39.0	36.0	41.0	31.0	41.0
38	37.12482405937026	39.0	36.0	41.0	31.0	41.0
39	37.031155653628765	39.0	36.0	41.0	31.0	41.0
40	36.951044708552814	39.0	36.0	40.0	31.0	41.0
41	36.903026246637296	39.0	36.0	40.0	31.0	41.0
42	36.76826925459609	39.0	36.0	40.0	30.0	41.0
43	35.47760208131739	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	8.0
11	10.0
12	3.0
13	3.0
14	12.0
15	16.0
16	41.0
17	75.0
18	161.0
19	335.0
20	837.0
21	1846.0
22	3520.0
23	6414.0
24	11102.0
25	17893.0
26	28055.0
27	42383.0
28	62004.0
29	87052.0
30	120380.0
31	160919.0
32	213354.0
33	282077.0
34	382125.0
35	525906.0
36	761368.0
37	1188656.0
38	2147161.0
39	2067681.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.94279126380264	18.240671371073873	12.561146958460073	26.25539040666341
2	18.63495590958948	20.273365846417903	35.6940311544208	25.39764708957183
3	20.161954759726463	21.901765182118353	29.75559955672269	28.18068050143249
4	15.233804863056333	15.388044556051414	33.70596522105121	35.67218535984104
5	15.771862345358095	35.10429332737957	33.694573847353865	15.429270479908464
6	35.81360359326336	33.627063931372646	15.028303988423207	15.53102848694079
7	29.5154807412431	29.76977713221181	21.068851114622493	19.6458910119226
8	26.343303703022446	34.67934824082794	19.48033391518925	19.49701414096036
9	26.37244786689747	14.909495905824407	19.650526462691218	39.06752976458691
10	17.562342189715437	26.23298983739061	32.913364781250486	23.29130319164347
11	35.74290064071546	21.573548145382038	21.308106947246227	21.375444266656277
12	21.529351094835526	24.536291572812143	30.090708127979372	23.843649204372955
13	30.921255156784877	19.6561358512543	24.053132128469546	25.369476863491276
14	22.392136697470637	20.540890531734284	25.322160746337165	31.744812024457918
15	25.289539994385677	27.285693309947316	22.392383264000877	25.032383431666123
16	24.736158402229158	25.915954592800922	24.476092354452703	24.871794650517216
17	24.14003450205458	26.096996067633693	25.40149352744366	24.361475902868072
18	24.305850493644687	25.209788050177767	26.026613651575108	24.457747804602437
19	25.08284327208086	25.086196576892206	26.2662270056677	23.564733145359227
20	24.659069376547897	24.980542818682	26.162138944924557	24.198248859845542
21	24.399397835219837	25.164049958817223	26.3329109237726	24.10364128219034
22	24.89802932933534	24.853499413972997	25.781119685736165	24.467351570955497
23	24.381250538593765	25.060689269338305	25.843217466378498	24.714842725689433
24	24.400260818075694	25.133796245556102	25.846792681067054	24.61915025530115
25	24.7690380490374	24.828103061357712	25.7534425927161	24.649416296888788
26	24.384135366997636	25.2174562692684	25.96789383239714	24.43051453133682
27	24.58258443886574	25.41019732596132	25.69283653958176	24.31438169559118
28	24.413057620995435	25.354793826615158	25.712833085184666	24.51931546720474
29	24.301535579365392	25.55753315610953	26.01700988522205	24.12392137930303
30	24.499331249928343	25.377490275724256	25.84590504155817	24.277273432789233
31	24.491810970755854	24.858800594373278	26.090671636132896	24.558716798737972
32	24.081672204345463	25.34196003871588	25.98458638649476	24.591781370443897
33	24.14152622956256	24.94894531783104	25.998381783862	24.9111466687444
34	24.451965819468178	24.7737597980916	26.296751942112095	24.477522440328126
35	24.181248097585115	24.879869704382756	26.323085247542316	24.61579695048981
36	24.08488989756517	24.563697442648934	26.54282533929712	24.80858732048878
37	23.911233090313253	24.16854992127747	26.96558831205608	24.954628676353195
38	23.67796882437448	24.19236824809919	27.312482763458494	24.817180164067835
39	23.39577343050849	24.06866581987501	27.82307273429091	24.71248801532559
40	23.550715838114773	23.981936042861154	27.859700192358876	24.607647926665198
41	23.0936924459782	24.077012096923823	27.974526225494216	24.85476923160376
42	22.77396962620884	24.38165737336867	27.89632765042685	24.948045349995642
43	22.719120901555723	24.117128471394768	27.813000491530378	25.350750135519128
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3391.0
1	2995.0
2	2599.0
3	4973.5
4	7348.0
5	7348.0
6	8974.5
7	10601.0
8	9678.5
9	8756.0
10	12477.5
11	16199.0
12	16199.0
13	25785.5
14	35372.0
15	42875.0
16	50378.0
17	47050.0
18	43722.0
19	43722.0
20	51590.0
21	59458.0
22	54199.5
23	48941.0
24	58504.5
25	68068.0
26	68068.0
27	82456.5
28	96845.0
29	112190.0
30	127535.0
31	148989.5
32	170444.0
33	170444.0
34	197223.0
35	224002.0
36	256735.0
37	289468.0
38	326683.0
39	363898.0
40	363898.0
41	404624.0
42	445350.0
43	489121.0
44	532892.0
45	574188.5
46	615485.0
47	615485.0
48	654593.5
49	693702.0
50	711074.0
51	728446.0
52	721425.0
53	714404.0
54	714404.0
55	686190.5
56	657977.0
57	614654.0
58	571331.0
59	521990.5
60	472650.0
61	472650.0
62	415509.5
63	358369.0
64	305930.0
65	253491.0
66	210547.5
67	167604.0
68	167604.0
69	136615.5
70	105627.0
71	85922.5
72	66218.0
73	54134.0
74	42050.0
75	42050.0
76	33897.5
77	25745.0
78	20869.5
79	15994.0
80	12312.5
81	8631.0
82	8631.0
83	6600.0
84	4569.0
85	3431.0
86	2293.0
87	1684.0
88	1075.0
89	1075.0
90	690.0
91	305.0
92	202.5
93	100.0
94	75.5
95	51.0
96	51.0
97	32.5
98	14.0
99	8.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8111401.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.17886675113247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.81739969998722	41.126025966163816
2	10.57231447157086	11.033027761263305
3	3.7860808450142747	5.926602237630445
4	1.8493297113909684	3.8598371435831833
5	1.113745827648125	2.905699756774063
6	0.7065143424485285	2.211907063943144
7	0.4868312566991432	1.7781612291513673
8	0.3649756327334257	1.5235211926246144
9	0.2982263282073158	1.4005000657088127
>10	1.8157156580720937	17.873383050946373
>50	0.14097921569749608	5.02561815188256
>100	0.046823055364085084	3.92228218017153
>500	7.803010772518046E-4	0.26699286249250115
>1k	1.8910272671559747E-4	0.27296281144748596
>5k	0.0	0.0
>10k+	9.455136247373657E-5	0.8734785262167812
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23631	0.29133068381158816	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20351	0.25089377285132375	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	15177	0.187107011476809	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11660	0.1437482871331352	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4656653024551493E-5
2	0.0	0.0	0.0	0.0	3.698497953682724E-5
3	0.0	0.0	0.0	0.0	3.698497953682724E-5
4	0.0	0.0	0.0	0.0	6.164163256137873E-5
5	0.0	1.2328326512275746E-5	0.0	0.0	6.164163256137873E-5
6	0.0	1.2328326512275746E-5	0.0	0.0	1.2328326512275745E-4
7	1.2328326512275746E-5	1.2328326512275746E-5	0.0	0.0	1.2328326512275745E-4
8	1.2328326512275746E-5	1.2328326512275746E-5	0.0	0.0	1.356115916350332E-4
9	1.2328326512275746E-5	1.2328326512275746E-5	0.0	4.9313306049102985E-5	2.095815507086877E-4
10	2.4656653024551493E-5	1.2328326512275746E-5	0.0	4.9313306049102985E-5	2.095815507086877E-4
11	3.698497953682724E-5	1.2328326512275746E-5	0.0	4.9313306049102985E-5	2.095815507086877E-4
12	3.698497953682724E-5	1.2328326512275746E-5	0.0	4.9313306049102985E-5	2.095815507086877E-4
13	3.698497953682724E-5	1.2328326512275746E-5	0.0	6.164163256137873E-5	2.2190987722096344E-4
14	4.9313306049102985E-5	2.4656653024551493E-5	0.0	8.629828558593022E-5	3.205364893191694E-4
15	4.9313306049102985E-5	2.4656653024551493E-5	0.0	1.1095493861048172E-4	4.438197544419269E-4
16	4.9313306049102985E-5	2.4656653024551493E-5	0.0	2.3423820373323918E-4	5.177897135155813E-4
17	4.9313306049102985E-5	2.4656653024551493E-5	0.0	3.3286481583144514E-4	5.177897135155813E-4
18	4.9313306049102985E-5	2.4656653024551493E-5	0.0	3.5752146885599664E-4	5.424463665401328E-4
19	7.396995907365447E-5	2.4656653024551493E-5	0.0	4.0683477490509963E-4	5.424463665401328E-4
20	7.396995907365447E-5	2.4656653024551493E-5	0.0	4.931330604910298E-4	5.424463665401328E-4
21	7.396995907365447E-5	2.4656653024551493E-5	0.0	7.520279172488205E-4	5.547746930524086E-4
22	7.396995907365447E-5	2.4656653024551493E-5	1.2328326512275746E-5	0.0011711910186661958	5.671030195646843E-4
23	7.396995907365447E-5	2.4656653024551493E-5	1.2328326512275746E-5	0.0016643240791572257	6.903862846874418E-4
24	7.396995907365447E-5	2.4656653024551493E-5	1.2328326512275746E-5	0.0028355150978234215	6.903862846874418E-4
25	7.396995907365447E-5	2.4656653024551493E-5	1.2328326512275746E-5	0.0041423177081246505	7.027146111997176E-4
26	7.396995907365447E-5	2.4656653024551493E-5	1.2328326512275746E-5	0.0073600109278286205	7.150429377119933E-4
27	7.396995907365447E-5	2.4656653024551493E-5	1.2328326512275746E-5	0.02787434624425546	7.273712642242691E-4
28	7.396995907365447E-5	2.4656653024551493E-5	1.2328326512275746E-5	0.08187241636802323	7.273712642242691E-4
29	7.396995907365447E-5	2.4656653024551493E-5	1.2328326512275746E-5	0.15697658148080706	7.396995907365448E-4
30	7.396995907365447E-5	2.4656653024551493E-5	1.2328326512275746E-5	0.2517444273806707	7.396995907365448E-4
31	7.396995907365447E-5	2.4656653024551493E-5	1.2328326512275746E-5	0.5233991020786668	7.520279172488205E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10520	0.0	17.374525	1
CTTATAC	5210	0.0	14.84261	37
TCTTATA	8145	0.0	13.718846	37
CTCTTAT	12720	0.0	13.133254	37
GTATCAA	14260	0.0	12.778751	2
TCGTTAC	660	0.0	12.613637	23
ATACCGA	295	1.6370905E-11	11.915254	6
TCTCTTA	18470	0.0	11.498647	37
TAGACGG	265	5.3714757E-9	11.169811	5
TATACCG	300	2.8194336E-10	11.1	5
GGGTAAG	2145	0.0	10.694638	1
AACGCAG	16875	0.0	10.677926	7
CTACACT	1595	0.0	10.670847	4
GTATTAT	890	0.0	10.601123	1
CTATACT	1030	0.0	10.597087	4
TATACTG	1205	0.0	10.593362	5
TAGACAG	1695	0.0	10.587021	5
TAGGACT	1215	0.0	10.506173	4
GTCCTAC	850	0.0	10.447059	1
GTATTAC	695	0.0	10.381295	1
>>END_MODULE