FastQCFastQC Report
Thu 9 Feb 2017
SRR2727075.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727075.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2767053
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT134600.4864380985835833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT133300.4817399594442173No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT84230.30440327669907297No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61700.22298091146067672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC16550.014.75528737
ATACCGT2355.638867E-1113.3829796
CTCTTAT36850.013.35413837
GGTATCA83000.013.1728921
TATACTG3700.012.55
GTATTAT3150.012.3333341
TCTCTTA53550.012.26423837
TCTTATA23600.011.83686437
TATACCG1603.767138E-511.5625015
GTATTAG2603.979949E-911.3846151
ACCCGAC5250.011.27619111
TACCGTG2801.0604708E-911.2321437
GTACTAA1655.1561026E-511.2121211
CAATACT4850.011.0618554
GGGTAAG6700.011.0447761
TATAACG1350.001376033310.9629632
GTAAGAG6600.010.9318183
AATACCG1200.007166664610.7916665
TACCGAC1200.007166664610.7916667
AGTACTA1901.8286002E-510.7105265
Produced by FastQC (version 0.11.5)