##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727075.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2767053
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1624012984211	31.0	31.0	34.0	28.0	34.0
2	31.398347266929836	31.0	31.0	34.0	30.0	34.0
3	31.470804498504364	31.0	31.0	34.0	28.0	34.0
4	35.27065039954059	37.0	35.0	37.0	33.0	37.0
5	34.61572257560661	35.0	35.0	37.0	30.0	37.0
6	34.64079148465895	35.0	35.0	37.0	31.0	37.0
7	34.612288597291055	35.0	35.0	37.0	31.0	37.0
8	34.552401056286236	35.0	35.0	37.0	30.0	37.0
9	36.24485074915442	37.0	35.0	39.0	32.0	39.0
10	35.93406523113218	37.0	35.0	39.0	30.0	39.0
11	36.08924657388203	37.0	35.0	39.0	32.0	39.0
12	35.94097474822492	37.0	35.0	39.0	31.0	39.0
13	36.076235619628534	37.0	35.0	39.0	32.0	39.0
14	36.997554437880304	38.0	36.0	40.0	32.0	41.0
15	36.9207405857423	38.0	36.0	40.0	31.0	41.0
16	36.8416242840307	38.0	36.0	40.0	31.0	41.0
17	36.79914443272319	38.0	36.0	40.0	31.0	41.0
18	36.78995667954318	38.0	36.0	40.0	31.0	41.0
19	36.839892839060184	38.0	36.0	40.0	31.0	41.0
20	36.73720597328638	38.0	36.0	40.0	31.0	41.0
21	36.65482048952441	38.0	36.0	40.0	31.0	41.0
22	36.56578569329897	38.0	35.0	40.0	30.0	41.0
23	36.47902262804507	38.0	35.0	40.0	30.0	41.0
24	36.35901336186911	38.0	35.0	40.0	30.0	41.0
25	36.312210138367426	38.0	35.0	40.0	30.0	41.0
26	36.06779487057169	38.0	35.0	40.0	29.0	41.0
27	35.88224873177348	38.0	34.0	40.0	29.0	41.0
28	35.75938082862887	38.0	34.0	40.0	28.0	41.0
29	35.61330303394984	38.0	34.0	40.0	27.0	41.0
30	35.527430446760505	38.0	34.0	40.0	27.0	41.0
31	35.45357533809435	38.0	34.0	40.0	27.0	41.0
32	35.31380822846545	38.0	34.0	40.0	27.0	41.0
33	35.20333979869558	38.0	34.0	40.0	27.0	41.0
34	35.13363856781926	38.0	34.0	40.0	26.0	41.0
35	35.0206147117529	38.0	33.0	40.0	26.0	41.0
36	34.85280585518239	38.0	33.0	40.0	25.0	41.0
37	34.744607349407474	38.0	33.0	40.0	25.0	41.0
38	34.66337218694402	38.0	33.0	40.0	25.0	41.0
39	34.518029831737955	37.0	33.0	40.0	24.0	40.0
40	34.39130331077865	37.0	33.0	40.0	24.0	40.0
41	34.31131749193094	37.0	33.0	40.0	24.0	40.0
42	34.10498750837082	37.0	33.0	40.0	24.0	40.0
43	32.793188637875744	36.0	30.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	4.0
13	9.0
14	9.0
15	32.0
16	71.0
17	163.0
18	356.0
19	713.0
20	1441.0
21	2850.0
22	4910.0
23	8315.0
24	13252.0
25	19739.0
26	28612.0
27	39752.0
28	53550.0
29	69983.0
30	90025.0
31	112343.0
32	140722.0
33	176283.0
34	225040.0
35	291967.0
36	387593.0
37	490693.0
38	475776.0
39	132845.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.84173559378877	19.71509038677611	13.49815128224866	24.94502273718646
2	19.065482301929165	21.770959934630817	34.89665720172328	24.266900561716742
3	20.74951220666897	23.1377570288679	30.106217698034698	26.006513066428433
4	15.803564297467378	16.45902698647261	33.60969233332358	34.12771638273643
5	15.450806327164676	35.82204605405101	33.650204748517645	15.076942870266672
6	34.848808461565426	34.54888648681467	14.891908467239334	15.710396584380568
7	28.591790616226	30.414451765108947	21.520151583652357	19.4736060350127
8	26.29064206576455	34.95357696437329	19.531465425490584	19.224315544371574
9	25.970301255523477	15.117925099374677	20.124515143005933	38.78725850209591
10	17.553837964072248	26.98784591404646	32.79362556481571	22.664690557065587
11	34.923436594817666	22.295344541647736	21.349067039915752	21.43215182361885
12	21.136964127539297	25.668500025116973	30.070692538234724	23.123843309109006
13	30.95137678967479	20.078654077099355	24.414096874906264	24.555872258319592
14	22.003626240624953	21.38043615355398	26.07286524688902	30.54307235893205
15	25.03482947381203	27.89230997743809	22.916799931190333	24.15606061755955
16	23.892639569968484	26.28890736823617	25.372408840741397	24.44604422105395
17	23.493839836099994	26.847082437524687	26.07452766535372	23.5845500610216
18	23.644035730432343	25.51996654924933	26.868187924119997	23.96780979619834
19	24.44550212807633	25.59022179914877	27.312668026235855	22.651608046539042
20	24.050460905519337	25.594377845310518	27.08719348707813	23.267967762092017
21	23.66019010116539	25.86152126468123	27.257193844859493	23.22109478929388
22	24.0935030879423	25.754548250431053	26.694320636431613	23.457628025195035
23	23.712520143271558	25.75382545979423	26.71448649519904	23.819167901735167
24	23.62900168518637	25.871134380151013	26.76959928125699	23.730264653405627
25	24.006226118545616	25.595787287052325	26.635124083275603	23.76286251112646
26	23.56304703957604	25.892673541128413	27.026515213116625	23.51776420617892
27	23.87836445489118	26.088983478090228	26.721063889994156	23.311588177024436
28	23.706520980985907	26.028811157574506	26.77523704822423	23.48943081321536
29	23.461567234165734	26.280559136380838	27.000783866445637	23.257089763007794
30	23.56496243476363	26.288509833385916	26.819507974729795	23.327019757120663
31	23.695968237688255	25.656429421481985	27.073713441701337	23.573888899128423
32	23.23551446249855	26.21305049090133	26.827205695011987	23.724229351588132
33	23.399334960335057	25.86065391591704	26.801474348341	23.938536775406906
34	23.65213098556479	25.815913175497542	27.00273540116507	23.529220437772604
35	23.420368167866677	25.92444018961689	26.98784591404646	23.667345728469964
36	23.38863765891004	25.618229936325758	27.161677062202997	23.8314553425612
37	23.209927673954926	25.275591034938614	27.486463034860552	24.028018256245907
38	22.992476110865965	25.353146470269994	27.83596844729754	23.8184089715665
39	22.756412688878747	25.24548680491483	28.18511246441611	23.81298804179031
40	22.85991630807216	25.19727666943857	28.2674021784187	23.67540484407057
41	22.593459539806428	25.146319929542372	28.309613151609312	23.95060737904189
42	22.28179221720726	25.45618027554947	28.134553259370165	24.1274742478731
43	22.352625699616162	25.108373421109025	28.033976942255894	24.505023937018915
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1572.0
1	1263.0
2	954.0
3	1704.0
4	2454.0
5	2454.0
6	3354.5
7	4255.0
8	4370.5
9	4486.0
10	6557.5
11	8629.0
12	8629.0
13	14183.0
14	19737.0
15	26094.5
16	32452.0
17	28788.0
18	25124.0
19	25124.0
20	28380.5
21	31637.0
22	26714.5
23	21792.0
24	25432.5
25	29073.0
26	29073.0
27	34428.0
28	39783.0
29	45087.5
30	50392.0
31	58412.5
32	66433.0
33	66433.0
34	76043.0
35	85653.0
36	97249.0
37	108845.0
38	121461.0
39	134077.0
40	134077.0
41	146471.5
42	158866.0
43	171809.5
44	184753.0
45	196125.0
46	207497.0
47	207497.0
48	218553.0
49	229609.0
50	233419.5
51	237230.0
52	233834.5
53	230439.0
54	230439.0
55	218203.0
56	205967.0
57	191399.5
58	176832.0
59	161047.5
60	145263.0
61	145263.0
62	127293.0
63	109323.0
64	92979.5
65	76636.0
66	63886.5
67	51137.0
68	51137.0
69	42025.5
70	32914.0
71	26899.0
72	20884.0
73	16981.5
74	13079.0
75	13079.0
76	10707.5
77	8336.0
78	6695.5
79	5055.0
80	3999.0
81	2943.0
82	2943.0
83	2252.0
84	1561.0
85	1180.0
86	799.0
87	603.0
88	407.0
89	407.0
90	267.0
91	127.0
92	79.5
93	32.0
94	22.0
95	12.0
96	12.0
97	6.0
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2767053.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.34440779232726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.00870707829152	53.21481799273467
2	8.617278593447375	10.917128185668455
3	2.742359066733289	5.211393331084184
4	1.3399455077850084	3.395122186585224
5	0.7700863614869959	2.43903322586709
6	0.5104694175887975	1.9401229771953952
7	0.3475433737896955	1.5410450436398988
8	0.2563287968095995	1.2989596667219094
9	0.2071185255182697	1.1807820307597332
>10	1.1267884566996382	12.684577347461975
>50	0.05519617053169236	2.3543856686457896
>100	0.016923105114662062	1.696818810391083
>500	6.848433837132584E-4	0.296400672738584
>1k	3.424216918380402E-4	0.3333829740062607
>5k	1.141405639460134E-4	0.5275490934367125
>10k+	1.141405639460134E-4	0.96848079306308
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13460	0.4864380985835833	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13330	0.4817399594442173	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8423	0.30440327669907297	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6170	0.22298091146067672	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.613953184127662E-5	0.0
5	0.0	0.0	0.0	3.613953184127662E-5	0.0
6	0.0	0.0	0.0	3.613953184127662E-5	0.0
7	3.613953184127662E-5	0.0	0.0	3.613953184127662E-5	0.0
8	3.613953184127662E-5	0.0	0.0	3.613953184127662E-5	3.613953184127662E-5
9	3.613953184127662E-5	0.0	0.0	1.0841859552382987E-4	3.613953184127662E-5
10	3.613953184127662E-5	0.0	0.0	1.0841859552382987E-4	3.613953184127662E-5
11	3.613953184127662E-5	0.0	0.0	1.0841859552382987E-4	3.613953184127662E-5
12	3.613953184127662E-5	0.0	0.0	1.4455812736510648E-4	7.227906368255324E-5
13	3.613953184127662E-5	0.0	0.0	1.8069765920638312E-4	7.227906368255324E-5
14	3.613953184127662E-5	0.0	0.0	1.8069765920638312E-4	1.4455812736510648E-4
15	7.227906368255324E-5	0.0	0.0	2.1683719104765973E-4	2.1683719104765973E-4
16	1.0841859552382987E-4	0.0	0.0	2.5297672288893634E-4	2.8911625473021296E-4
17	1.0841859552382987E-4	0.0	0.0	3.2525578657148957E-4	2.8911625473021296E-4
18	1.0841859552382987E-4	0.0	0.0	3.6139531841276624E-4	2.8911625473021296E-4
19	1.0841859552382987E-4	0.0	0.0	6.143720413017026E-4	2.8911625473021296E-4
20	1.0841859552382987E-4	0.0	0.0	7.227906368255325E-4	3.9753485025404285E-4
21	1.0841859552382987E-4	0.0	0.0	0.0011926045507621285	4.3367438209531946E-4
22	1.0841859552382987E-4	0.0	0.0	0.0018792556557463843	4.3367438209531946E-4
23	1.0841859552382987E-4	0.0	0.0	0.002385209101524257	5.782325094604259E-4
24	1.0841859552382987E-4	0.0	0.0	0.003650092715968939	5.782325094604259E-4
25	1.0841859552382987E-4	0.0	0.0	0.0045174414801595775	5.782325094604259E-4
26	1.4455812736510648E-4	0.0	0.0	0.006468976199588515	5.782325094604259E-4
27	1.4455812736510648E-4	0.0	0.0	0.019551486726130652	5.782325094604259E-4
28	1.4455812736510648E-4	0.0	0.0	0.058473762519185575	5.782325094604259E-4
29	1.4455812736510648E-4	0.0	0.0	0.12999389603307202	5.782325094604259E-4
30	1.4455812736510648E-4	0.0	0.0	0.21709016777054865	5.782325094604259E-4
31	1.4455812736510648E-4	0.0	0.0	0.4424201488009084	5.782325094604259E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1655	0.0	14.755287	37
ATACCGT	235	5.638867E-11	13.382979	6
CTCTTAT	3685	0.0	13.354138	37
GGTATCA	8300	0.0	13.172892	1
TATACTG	370	0.0	12.5	5
GTATTAT	315	0.0	12.333334	1
TCTCTTA	5355	0.0	12.264238	37
TCTTATA	2360	0.0	11.836864	37
TATACCG	160	3.767138E-5	11.562501	5
GTATTAG	260	3.979949E-9	11.384615	1
ACCCGAC	525	0.0	11.276191	11
TACCGTG	280	1.0604708E-9	11.232143	7
GTACTAA	165	5.1561026E-5	11.212121	1
CAATACT	485	0.0	11.061855	4
GGGTAAG	670	0.0	11.044776	1
TATAACG	135	0.0013760333	10.962963	2
GTAAGAG	660	0.0	10.931818	3
AATACCG	120	0.0071666646	10.791666	5
TACCGAC	120	0.0071666646	10.791666	7
AGTACTA	190	1.8286002E-5	10.710526	5
>>END_MODULE