FastQCFastQC Report
Thu 9 Feb 2017
SRR2727074.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727074.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7338045
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT241820.3295428141964242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT236170.321843215733891No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT165190.22511445487183576No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121150.16509846968777106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC49450.016.19919237
GGTATCA116550.016.0476191
TCTTATA81200.014.85467937
CTCTTAT122950.012.60919137
GTTCGAC3450.012.33333411
GTATCAA155450.011.99614052
CGTATAC2502.1518645E-911.843
GGACCGT5050.011.7227726
TAGTACT7600.010.9539474
TCTCTTA176450.010.85151737
CGCGAAA2401.8466017E-710.79166710
GTCTTAG10550.010.6966831
TCTATAC8500.010.6647063
TATACCG3503.45608E-1110.5714285
GTATTAG6900.010.4565221
AACGCAG177400.010.4284117
TAGACAG14950.010.2709035
GGGTAAG20350.010.0909091
GTACTAC6250.010.0641
TAAGACT11300.09.9867264
Produced by FastQC (version 0.11.5)