##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727074.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7338045
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.751131806904972	31.0	31.0	34.0	30.0	34.0
2	31.9866311803757	33.0	31.0	34.0	30.0	34.0
3	32.08645395333498	33.0	31.0	34.0	30.0	34.0
4	35.75079983837657	37.0	35.0	37.0	35.0	37.0
5	35.40371693005426	37.0	35.0	37.0	33.0	37.0
6	35.41463850385218	37.0	35.0	37.0	33.0	37.0
7	35.38193292627668	37.0	35.0	37.0	33.0	37.0
8	35.34458169716866	37.0	35.0	37.0	33.0	37.0
9	37.123536037187016	39.0	37.0	39.0	33.0	39.0
10	36.91898741422273	39.0	37.0	39.0	33.0	39.0
11	37.01212530040358	39.0	37.0	39.0	33.0	39.0
12	36.907808823739835	39.0	37.0	39.0	33.0	39.0
13	36.9893084602234	39.0	37.0	39.0	33.0	39.0
14	38.122726012173544	40.0	37.0	41.0	33.0	41.0
15	38.061781032959054	40.0	37.0	41.0	33.0	41.0
16	38.01696787087024	40.0	37.0	41.0	33.0	41.0
17	37.96458511769824	40.0	37.0	41.0	33.0	41.0
18	37.96077388459733	40.0	37.0	41.0	33.0	41.0
19	38.00642923285425	40.0	37.0	41.0	33.0	41.0
20	37.91836054425941	40.0	37.0	41.0	33.0	41.0
21	37.85566714295156	40.0	37.0	41.0	33.0	41.0
22	37.78597978071816	39.0	37.0	41.0	33.0	41.0
23	37.72507772846855	39.0	37.0	41.0	32.0	41.0
24	37.6342993263192	39.0	37.0	41.0	32.0	41.0
25	37.59814773553447	39.0	37.0	41.0	32.0	41.0
26	37.41591909016638	39.0	37.0	41.0	32.0	41.0
27	37.2734597566518	39.0	36.0	40.0	31.0	41.0
28	37.188685951094605	39.0	36.0	40.0	31.0	41.0
29	37.086828440000026	39.0	36.0	40.0	31.0	41.0
30	37.0232200810979	39.0	36.0	40.0	31.0	41.0
31	36.98234189078971	39.0	36.0	40.0	31.0	41.0
32	36.867780042231956	39.0	36.0	40.0	30.0	41.0
33	36.7792166714704	39.0	36.0	40.0	30.0	41.0
34	36.73784393527159	39.0	35.0	40.0	30.0	41.0
35	36.65679877951143	39.0	35.0	40.0	30.0	41.0
36	36.53206828249213	39.0	35.0	40.0	30.0	41.0
37	36.44601211903171	39.0	35.0	40.0	30.0	41.0
38	36.404176725544744	39.0	35.0	40.0	30.0	41.0
39	36.30114369699287	39.0	35.0	40.0	30.0	41.0
40	36.20969508908708	38.0	35.0	40.0	30.0	41.0
41	36.15583701108402	38.0	35.0	40.0	29.0	41.0
42	36.007728625267355	38.0	35.0	40.0	29.0	41.0
43	34.70420364007035	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	15.0
11	23.0
12	10.0
13	9.0
14	13.0
15	16.0
16	53.0
17	136.0
18	270.0
19	588.0
20	1390.0
21	2767.0
22	5432.0
23	9594.0
24	16237.0
25	25828.0
26	39555.0
27	57064.0
28	81448.0
29	110434.0
30	148225.0
31	192311.0
32	246476.0
33	317083.0
34	414368.0
35	554090.0
36	778524.0
37	1169511.0
38	1910695.0
39	1255872.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.33029369539162	18.755554101944046	12.976998642008871	25.937153560655464
2	18.646683142444616	20.642323670677953	35.70181158605596	25.009181600821474
3	20.22908008877024	22.273670984574228	29.833763625052722	27.663485301602815
4	15.3693797189851	15.743989032501164	33.70377259883252	35.182858649681215
5	15.673806851824976	35.351513925030446	33.64727253648622	15.327406686658367
6	35.5365768402892	33.973122268942205	15.011614128831315	15.478686761937274
7	29.32426824855939	29.954994824915897	21.140835195205263	19.579901731319445
8	26.27022592529754	34.94557474095621	19.421521672325532	19.36267766142072
9	26.312689551508612	15.014435043666264	19.72092021785094	38.95195518697419
10	17.551159743501163	26.486809497625046	32.82243431322648	23.13959644564731
11	35.63742113873654	21.728621179074263	21.306506024424763	21.32745165776443
12	21.46239223117329	24.79447863838393	30.131758526964607	23.611370603478175
13	30.953966621899976	19.754226091554358	24.18033686083964	25.111470425706027
14	22.285826810819504	20.642310043070054	25.557270362882754	31.514592783227684
15	25.167847839581253	27.384405519453754	22.565015613831747	24.882731027133246
16	24.514240509563514	26.02548771505217	24.638646942066995	24.821624833317323
17	23.989931923284743	26.26070022737664	25.579156301167412	24.17021154817121
18	24.192560825124403	25.29240417577161	26.19100864058479	24.324026358519195
19	24.980958824864118	25.193794805019593	26.511679882039424	23.313566488076866
20	24.532637780226203	25.1093036360502	26.383117029126968	23.974941554596626
21	24.23900371284177	25.280520901684305	26.58146686208656	23.899008523387362
22	24.744874145634157	25.069756317929365	26.016057410386555	24.16931212604992
23	24.24420945905892	25.222603568116575	26.03395045955701	24.499236513267498
24	24.22262332814803	25.280139328663154	26.090082576490058	24.40715476669876
25	24.604141838868525	24.97032929070345	25.988338855921434	24.437190014506587
26	24.21585040702258	25.347664125799174	26.20587636080182	24.230609106376424
27	24.409839405454722	25.55121970497592	25.966793607834237	24.07214728173512
28	24.26162554195293	25.481964201636814	26.019736864519093	24.236673391891163
29	24.12002106828181	25.74553576599762	26.22507766033051	23.90936550539006
30	24.294795139577367	25.602037054828635	26.0650759159967	24.038091889597297
31	24.310739440818367	25.050282466242713	26.278606904154987	24.360371188783933
32	23.867269824592245	25.511658759247187	26.211245638313745	24.409825777846823
33	23.988228472297457	25.135155208233257	26.20588998840972	24.670726331059566
34	24.258845509941683	24.995472227275794	26.545612625706166	24.20006963707636
35	24.00246932255117	25.075071085009697	26.530663139841742	24.39179645259739
36	23.892140209006623	24.75832459462977	26.800353500148884	24.549181696214728
37	23.698614549243022	24.338703292225656	27.198579458152683	24.764102700378643
38	23.49050734902825	24.33499658287732	27.559847343536326	24.61464872455811
39	23.21239512703997	24.22863310323117	28.012447457054297	24.54652431267456
40	23.3587147530439	24.188881370991865	28.078745769479475	24.373658106484765
41	22.913814237988454	24.24163384116614	28.20204836574319	24.64250355510221
42	22.54303428229181	24.59225856478122	28.074507583423102	24.790199569503866
43	22.48856473352235	24.27668404868054	28.07191833792243	25.16283287987468
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3989.0
1	3403.5
2	2818.0
3	4951.5
4	7085.0
5	7085.0
6	8733.5
7	10382.0
8	9693.5
9	9005.0
10	12988.0
11	16971.0
12	16971.0
13	26923.0
14	36875.0
15	46440.5
16	56006.0
17	51264.5
18	46523.0
19	46523.0
20	53435.5
21	60348.0
22	53829.0
23	47310.0
24	56082.5
25	64855.0
26	64855.0
27	77889.0
28	90923.0
29	104912.5
30	118902.0
31	138072.5
32	157243.0
33	157243.0
34	182137.5
35	207032.0
36	237248.0
37	267464.0
38	301795.5
39	336127.0
40	336127.0
41	372564.5
42	409002.0
43	448177.0
44	487352.0
45	521847.0
46	556342.0
47	556342.0
48	590665.5
49	624989.0
50	638555.5
51	652122.0
52	644914.0
53	637706.0
54	637706.0
55	610848.0
56	583990.0
57	545781.5
58	507573.0
59	463007.0
60	418441.0
61	418441.0
62	366972.0
63	315503.0
64	268376.0
65	221249.0
66	183612.5
67	145976.0
68	145976.0
69	119445.5
70	92915.0
71	75288.5
72	57662.0
73	47242.0
74	36822.0
75	36822.0
76	29707.5
77	22593.0
78	18080.5
79	13568.0
80	10454.0
81	7340.0
82	7340.0
83	5610.5
84	3881.0
85	2885.5
86	1890.0
87	1366.5
88	843.0
89	843.0
90	571.5
91	300.0
92	191.5
93	83.0
94	61.0
95	39.0
96	39.0
97	21.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7338045.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.68071510051292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.17165098875087	42.499908407643765
2	10.613624645071901	11.394939215117745
3	3.6683653767675932	5.907614300245407
4	1.8024786476489645	3.870333710368074
5	1.0387695912015218	2.788094724018258
6	0.7201056274879716	2.3193471018874727
7	0.4956692658832876	1.8625516452172803
8	0.3631655581549103	1.5595989489305995
9	0.27220122609025177	1.3150760820984997
>10	1.6928200230456887	16.97033956176127
>50	0.11782582634352164	4.2905026878089965
>100	0.04228209737947975	3.611436954914297
>500	7.110536628564638E-4	0.2409278959017447
>1k	2.285117387087105E-4	0.32757470162804625
>5k	0.0	0.0
>10k+	1.0156077246912477E-4	1.0417540624585075
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	24182	0.3295428141964242	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23617	0.321843215733891	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16519	0.22511445487183576	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12115	0.16509846968777106	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.3627607898288985E-5	0.0	0.0	0.0	0.0
3	1.3627607898288985E-5	0.0	0.0	0.0	0.0
4	1.3627607898288985E-5	0.0	0.0	0.0	2.725521579657797E-5
5	1.3627607898288985E-5	0.0	0.0	0.0	2.725521579657797E-5
6	1.3627607898288985E-5	0.0	0.0	0.0	9.53932552880229E-5
7	1.3627607898288985E-5	0.0	0.0	0.0	9.53932552880229E-5
8	1.3627607898288985E-5	0.0	0.0	0.0	1.0902086318631188E-4
9	4.088282369486696E-5	0.0	0.0	0.0	2.3166933427091276E-4
10	4.088282369486696E-5	0.0	0.0	0.0	2.3166933427091276E-4
11	4.088282369486696E-5	0.0	0.0	2.725521579657797E-5	2.3166933427091276E-4
12	4.088282369486696E-5	0.0	0.0	4.088282369486696E-5	2.3166933427091276E-4
13	4.088282369486696E-5	0.0	0.0	6.813803949144492E-5	2.725521579657797E-4
14	4.088282369486696E-5	0.0	0.0	6.813803949144492E-5	3.952006290503806E-4
15	4.088282369486696E-5	0.0	0.0	9.53932552880229E-5	5.042214922366925E-4
16	4.088282369486696E-5	0.0	0.0	1.4990368688117884E-4	5.587319238298484E-4
17	4.088282369486696E-5	0.0	0.0	2.044141184743348E-4	5.587319238298484E-4
18	4.088282369486696E-5	0.0	0.0	2.1804172637262376E-4	6.404975712195823E-4
19	6.813803949144492E-5	0.0	0.0	2.4529694216920175E-4	6.404975712195823E-4
20	6.813803949144492E-5	0.0	0.0	3.2706258955893567E-4	6.404975712195823E-4
21	6.813803949144492E-5	0.0	0.0	6.950080028127383E-4	6.813803949144493E-4
22	6.813803949144492E-5	0.0	0.0	0.0011992294950494307	6.813803949144493E-4
23	6.813803949144492E-5	0.0	0.0	0.0016353129477946783	8.85794513388784E-4
24	6.813803949144492E-5	0.0	0.0	0.0025347350690817514	8.99422121287073E-4
25	6.813803949144492E-5	0.0	0.0	0.003515922837758558	8.99422121287073E-4
26	6.813803949144492E-5	0.0	0.0	0.0059961474752471535	8.99422121287073E-4
27	6.813803949144492E-5	0.0	0.0	0.02378017578251428	8.99422121287073E-4
28	6.813803949144492E-5	0.0	0.0	0.07488370540109798	9.13049729185362E-4
29	6.813803949144492E-5	0.0	0.0	0.15163439308426155	9.13049729185362E-4
30	6.813803949144492E-5	0.0	0.0	0.25295565780804014	9.13049729185362E-4
31	6.813803949144492E-5	0.0	0.0	0.5510186977594168	9.4030494498194E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4945	0.0	16.199192	37
GGTATCA	11655	0.0	16.047619	1
TCTTATA	8120	0.0	14.854679	37
CTCTTAT	12295	0.0	12.609191	37
GTTCGAC	345	0.0	12.333334	11
GTATCAA	15545	0.0	11.9961405	2
CGTATAC	250	2.1518645E-9	11.84	3
GGACCGT	505	0.0	11.722772	6
TAGTACT	760	0.0	10.953947	4
TCTCTTA	17645	0.0	10.851517	37
CGCGAAA	240	1.8466017E-7	10.791667	10
GTCTTAG	1055	0.0	10.696683	1
TCTATAC	850	0.0	10.664706	3
TATACCG	350	3.45608E-11	10.571428	5
GTATTAG	690	0.0	10.456522	1
AACGCAG	17740	0.0	10.428411	7
TAGACAG	1495	0.0	10.270903	5
GGGTAAG	2035	0.0	10.090909	1
GTACTAC	625	0.0	10.064	1
TAAGACT	1130	0.0	9.986726	4
>>END_MODULE