##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727073.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3420507
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.560723892686084	31.0	31.0	34.0	30.0	34.0
2	31.775780607962503	31.0	31.0	34.0	30.0	34.0
3	31.86665660967804	31.0	31.0	34.0	30.0	34.0
4	35.61574789936112	37.0	35.0	37.0	33.0	37.0
5	35.21961831974032	37.0	35.0	37.0	33.0	37.0
6	35.270011726331795	37.0	35.0	37.0	32.0	37.0
7	35.193970367550776	37.0	35.0	37.0	32.0	37.0
8	35.16488111265377	37.0	35.0	37.0	32.0	37.0
9	36.9833779027495	39.0	37.0	39.0	33.0	39.0
10	36.75428818008558	39.0	37.0	39.0	32.0	39.0
11	36.87277003087554	39.0	37.0	39.0	33.0	39.0
12	36.755532732428264	39.0	37.0	39.0	32.0	39.0
13	36.91243227977607	39.0	37.0	39.0	33.0	39.0
14	37.85484695689849	39.0	37.0	41.0	33.0	41.0
15	37.865888594877894	39.0	37.0	41.0	33.0	41.0
16	37.794093390248875	39.0	37.0	41.0	33.0	41.0
17	37.771354948257674	39.0	37.0	41.0	33.0	41.0
18	37.82869586292325	39.0	37.0	41.0	33.0	41.0
19	37.85981522622231	39.0	37.0	41.0	33.0	41.0
20	37.7425191645566	39.0	37.0	41.0	32.0	41.0
21	37.72385117177073	39.0	37.0	41.0	32.0	41.0
22	37.60804728655722	39.0	37.0	40.0	32.0	41.0
23	37.516651771214036	39.0	36.0	40.0	32.0	41.0
24	37.394785626809124	39.0	36.0	40.0	32.0	41.0
25	37.35867226700603	39.0	36.0	40.0	32.0	41.0
26	37.16265366508532	39.0	36.0	40.0	31.0	41.0
27	36.92150929672122	39.0	35.0	40.0	31.0	41.0
28	36.77146575054517	39.0	35.0	40.0	31.0	41.0
29	36.60799173923632	39.0	35.0	40.0	30.0	41.0
30	36.51579926601524	38.0	35.0	40.0	30.0	41.0
31	36.35937567150133	38.0	35.0	40.0	30.0	41.0
32	36.17457645898693	38.0	35.0	40.0	30.0	41.0
33	36.064560604612126	38.0	35.0	40.0	30.0	41.0
34	35.98428186230872	38.0	35.0	40.0	30.0	41.0
35	35.800463790894156	38.0	35.0	40.0	29.0	41.0
36	35.65397059558715	38.0	35.0	40.0	28.0	41.0
37	35.4451179313476	38.0	34.0	40.0	27.0	41.0
38	35.30078874272147	38.0	34.0	40.0	26.0	41.0
39	35.11328115978128	38.0	34.0	40.0	25.0	41.0
40	34.95042430844316	38.0	34.0	40.0	24.0	41.0
41	34.79464360108019	38.0	33.0	40.0	24.0	41.0
42	34.62368268797579	38.0	33.0	40.0	23.0	41.0
43	33.35269303644167	37.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	20.0
11	32.0
12	25.0
13	16.0
14	14.0
15	19.0
16	31.0
17	77.0
18	164.0
19	373.0
20	935.0
21	1854.0
22	3272.0
23	5696.0
24	9503.0
25	15188.0
26	23343.0
27	34499.0
28	48510.0
29	65566.0
30	85462.0
31	108681.0
32	138561.0
33	177569.0
34	229849.0
35	301682.0
36	410559.0
37	560390.0
38	742797.0
39	455815.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.86653586734364	18.268315194209514	13.526883587725447	23.3382653507214
2	18.10737414073411	22.71511211642017	35.5190911756649	23.658422567180832
3	21.28433007153618	24.418163740053743	29.986519542278383	24.3109866461317
4	15.162547540466953	16.019876585547113	33.017883021435125	35.799692852550805
5	15.666624859998823	35.96072161232238	32.84913610760042	15.52351742007837
6	34.437526366705285	33.34496903529214	14.851804133129972	17.365700464872607
7	27.621723914027953	30.84612895105901	22.34510848830305	19.18703864660999
8	26.810908441350946	34.20563091962683	20.06152889030778	18.921931748714446
9	26.239443450927013	15.21552214335477	20.687371784358284	37.857662621359935
10	18.32245921437962	27.024151682776854	31.77911929430345	22.874269808540078
11	33.087112524546804	22.950808169666075	21.38036846584439	22.581710839942733
12	21.34990514564069	26.7143438092657	30.51638251288479	21.41936853220882
13	31.183622778728413	19.727309431028793	25.752468859148657	23.336598931094134
14	21.525551621440915	21.96519404871851	28.546119040247543	27.963135289593033
15	24.926421726369803	27.487416339156738	25.264675675272702	22.321486259200757
16	22.266845236685672	26.07864857461189	27.62336109822316	24.031145090479278
17	22.538033104449134	26.859965496343086	27.92641558692907	22.675585812278705
18	22.63696580653102	24.76176777302312	29.205787329188333	23.395479091257524
19	23.86105334676994	25.224506191625977	30.074225838450264	20.84021462315382
20	23.15273729888581	25.5179714586171	29.401255427923406	21.928035814573686
21	22.027290106408202	26.6471900218301	29.875512606756832	21.450007265004867
22	22.37589339825938	26.47209901923896	29.05996099408655	22.09204658841511
23	21.900905333624518	26.245904481411674	29.364535725259444	22.488654459704367
24	22.005860534710205	26.45376840333904	29.433940640963456	22.106430420987298
25	22.243515361903953	25.979481989073548	29.26732791366894	22.50967473535356
26	21.740402811629973	26.57661568884379	29.962604958855515	21.720376540670724
27	22.342126474233208	26.70194798607341	29.695013049235097	21.260912490458285
28	22.196797141476395	27.039237165718415	29.322582880257226	21.441382812547964
29	21.925696980009103	27.122002673872615	29.660135178790746	21.292165167327536
30	21.640622282018427	27.167259122697306	29.768101629378336	21.42401696590593
31	21.39419682520749	26.708906018903043	29.883230760819963	22.013666395069503
32	21.085207543793945	27.01140503439987	29.622509177733008	22.280878244073175
33	21.482224711132005	26.842014941059904	29.42505306961804	22.250707278190045
34	21.699210087861243	27.030641948693575	29.328926969013658	21.941220994431525
35	21.227847216801486	27.07928970763691	29.580965628779595	22.11189744678201
36	21.103801278582385	26.86186579942681	29.689429081712156	22.344903840278647
37	20.744439347734122	26.310310138233895	29.896182057221342	23.04906845681064
38	20.768178518564646	26.273882789890507	30.273816133105413	22.684122558439437
39	20.28974067294702	26.216376695033805	30.644579882456025	22.84930274956315
40	20.674186604500445	26.3446033000371	30.523837548059397	22.45737254740306
41	20.354555625818044	26.24417959092029	30.650982442076568	22.750282341185095
42	19.84088908457138	26.682857248939996	30.445194235825273	23.03105943066335
43	19.827002254344166	26.273356552113476	30.207744056655926	23.691897136886432
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3496.0
1	3151.5
2	2807.0
3	6408.5
4	10010.0
5	10010.0
6	13138.0
7	16266.0
8	15962.5
9	15659.0
10	21898.0
11	28137.0
12	28137.0
13	44013.5
14	59890.0
15	78328.5
16	96767.0
17	86087.0
18	75407.0
19	75407.0
20	75690.5
21	75974.0
22	56623.5
23	37273.0
24	39553.0
25	41833.0
26	41833.0
27	46106.5
28	50380.0
29	55336.5
30	60293.0
31	65902.5
32	71512.0
33	71512.0
34	79157.5
35	86803.0
36	97844.0
37	108885.0
38	122555.0
39	136225.0
40	136225.0
41	153367.0
42	170509.0
43	189685.5
44	208862.0
45	229989.0
46	251116.0
47	251116.0
48	265187.0
49	279258.0
50	283249.5
51	287241.0
52	285885.5
53	284530.0
54	284530.0
55	263870.0
56	243210.0
57	220709.0
58	198208.0
59	180164.5
60	162121.0
61	162121.0
62	141650.0
63	121179.0
64	102750.0
65	84321.0
66	70312.0
67	56303.0
68	56303.0
69	46193.5
70	36084.0
71	29916.5
72	23749.0
73	19256.5
74	14764.0
75	14764.0
76	11974.0
77	9184.0
78	7466.0
79	5748.0
80	4472.0
81	3196.0
82	3196.0
83	2518.5
84	1841.0
85	1409.5
86	978.0
87	671.5
88	365.0
89	365.0
90	226.0
91	87.0
92	55.0
93	23.0
94	17.0
95	11.0
96	11.0
97	6.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3420507.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.31188000431343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.34773978829304	46.93467921589098
2	8.708138491819334	9.807432996245465
3	2.8597058257738452	4.831062339258386
4	1.3942212868354797	3.1404488721495594
5	0.834788230997933	2.350424734648434
6	0.5580207655601448	1.8853919034082796
7	0.38503355780521664	1.5177374453332866
8	0.2963784268118129	1.33517011251952
9	0.23074101441130324	1.1694114284042578
>10	1.2751546092172568	12.786551707086597
>50	0.06844803300327908	2.6102574747885345
>100	0.03568019143890033	3.9284975701528424
>500	0.003155969218245539	1.2134972879603136
>1k	0.002379911212133029	2.171225519447246
>5k	2.0694880105504598E-4	0.6912960634075042
>10k+	2.0694880105504598E-4	3.6269153292987273
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	40589	1.1866369517735236	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34076	0.996226582784365	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30273	0.8850442346704743	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19552	0.5716111675842207	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6205	0.18140585591551195	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6131	0.17924243394327213	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5856	0.17120269012751618	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5536	0.16184735186918195	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4952	0.144773859547722	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	2.923543205729443E-5	0.0	0.0	2.923543205729443E-5	0.0
5	2.923543205729443E-5	0.0	0.0	2.923543205729443E-5	0.0
6	2.923543205729443E-5	0.0	0.0	2.923543205729443E-5	0.0
7	5.847086411458886E-5	0.0	0.0	2.923543205729443E-5	0.0
8	1.1694172822917772E-4	0.0	0.0	2.923543205729443E-5	0.0
9	1.4617716028647216E-4	0.0	0.0	5.847086411458886E-5	8.770629617188329E-5
10	2.6311888851564985E-4	2.923543205729443E-5	0.0	2.0464802440106102E-4	8.770629617188329E-5
11	3.2158975263023873E-4	2.923543205729443E-5	0.0	4.970023449740053E-4	8.770629617188329E-5
12	3.5082518468753315E-4	2.923543205729443E-5	0.0	9.062983937761273E-4	1.1694172822917772E-4
13	3.800606167448276E-4	2.923543205729443E-5	0.0	0.0011109464181771884	1.1694172822917772E-4
14	4.970023449740053E-4	2.923543205729443E-5	0.0	0.0016371841952084881	2.923543205729443E-4
15	5.847086411458886E-4	2.923543205729443E-5	0.0	0.0023388345645835545	3.2158975263023873E-4
16	6.431795052604775E-4	2.923543205729443E-5	0.0	0.004034489623906632	3.800606167448276E-4
17	6.431795052604775E-4	2.923543205729443E-5	0.0	0.0057301446832297084	3.800606167448276E-4
18	6.431795052604775E-4	2.923543205729443E-5	0.0	0.007016503693750663	4.6776691291671086E-4
19	6.724149373177719E-4	5.847086411458886E-5	0.0	0.00929686739421963	4.6776691291671086E-4
20	7.016503693750663E-4	5.847086411458886E-5	0.0	0.0126004712166939	4.6776691291671086E-4
21	7.016503693750663E-4	5.847086411458886E-5	0.0	0.02040633157599151	4.6776691291671086E-4
22	7.016503693750663E-4	5.847086411458886E-5	0.0	0.030638732796044562	4.6776691291671086E-4
23	7.016503693750663E-4	5.847086411458886E-5	0.0	0.039350891549118305	5.554732090885942E-4
24	7.016503693750663E-4	5.847086411458886E-5	0.0	0.052740719431359155	5.554732090885942E-4
25	7.016503693750663E-4	5.847086411458886E-5	0.0	0.06206682225763607	5.554732090885942E-4
26	7.016503693750663E-4	5.847086411458886E-5	0.0	0.07715230519920001	5.554732090885942E-4
27	7.016503693750663E-4	5.847086411458886E-5	0.0	0.11568460465071406	5.554732090885942E-4
28	7.016503693750663E-4	5.847086411458886E-5	0.0	0.22976126053827692	5.554732090885942E-4
29	7.016503693750663E-4	5.847086411458886E-5	0.0	0.44119190517663026	5.554732090885942E-4
30	7.308858014323607E-4	5.847086411458886E-5	0.0	0.6742275341053241	5.554732090885942E-4
31	7.308858014323607E-4	5.847086411458886E-5	0.0	1.1710252310549285	5.554732090885942E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCCG	985	0.0	15.213197	5
ATACCGA	100	1.09436754E-4	14.799999	6
CTTATAC	4265	0.0	14.227432	37
TACCGAC	105	1.6571417E-4	14.095238	7
ACCCGTT	1020	0.0	13.784313	7
CTCCATA	1250	0.0	13.616	1
CATACCC	1160	0.0	13.556034	4
CCATACC	1190	0.0	13.525209	3
TACCCGT	1030	0.0	13.470873	6
TCCATAC	1195	0.0	13.3138075	2
TATACAC	1900	0.0	13.144738	37
GTAGGAC	310	0.0	13.129032	3
CCGTTAA	100	0.0018337677	12.949999	14
TTATCGT	125	7.225107E-4	11.840001	4
TAATACC	255	2.935849E-9	11.607843	4
GGGTAAG	1180	0.0	11.601695	1
CCCGTTT	1165	0.0	11.592275	8
TCTTATA	6205	0.0	11.538276	37
GTATATA	435	0.0	11.482759	1
ATACCGT	145	1.940345E-4	11.4827585	6
>>END_MODULE