FastQCFastQC Report
Thu 9 Feb 2017
SRR2727072.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727072.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3564002
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT240390.6744945709907009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT206020.5780580370044686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT179680.5041523545721915No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125510.3521602962063433No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43590.1223063286720939No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42610.11955661079875939No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40680.11414135008902912No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40250.11293484122623948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC35400.015.103106537
TCGTTAC3300.014.57575723
GTACTAA1651.6483682E-814.5757571
CGTACGA800.006302044313.87500110
CGTATAC950.001245972113.6315793
GTCCTAC2900.013.3965511
GTCTTAA4300.012.9069781
CGTTACA2451.1459633E-1012.83673424
CGTTACG2052.0696461E-812.63414624
TATACTG5000.012.5800015
TCTTATA52450.012.34509137
ATACCCG5000.012.2100015
GTCTTAG4700.012.20212651
ACCCGTT5350.012.1028037
ATACCGT2205.5342753E-811.7727276
TACCCGT5350.011.75700956
ACCGTGC4250.011.752948
CTCTTAT77350.011.62378837
GTCTAGA3850.011.5324671
GGCTAGT3053.092282E-1111.524591
Produced by FastQC (version 0.11.5)