##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727072.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3564002
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6129873103326	31.0	31.0	34.0	30.0	34.0
2	31.847887010164417	31.0	31.0	34.0	30.0	34.0
3	31.954782573073754	33.0	31.0	34.0	30.0	34.0
4	35.68820107283891	37.0	35.0	37.0	33.0	37.0
5	35.30077845074161	37.0	35.0	37.0	33.0	37.0
6	35.32397063750245	37.0	35.0	37.0	33.0	37.0
7	35.270502092872	37.0	35.0	37.0	32.0	37.0
8	35.227285225990336	37.0	35.0	37.0	32.0	37.0
9	37.04647864956305	39.0	37.0	39.0	33.0	39.0
10	36.820779842435556	39.0	37.0	39.0	33.0	39.0
11	36.93782354779823	39.0	37.0	39.0	33.0	39.0
12	36.82397709092195	39.0	37.0	39.0	33.0	39.0
13	36.95912628556325	39.0	37.0	39.0	33.0	39.0
14	37.967752543348745	40.0	37.0	41.0	33.0	41.0
15	37.97732577029979	40.0	37.0	41.0	33.0	41.0
16	37.9216366881949	39.0	37.0	41.0	33.0	41.0
17	37.85401607518739	39.0	37.0	41.0	33.0	41.0
18	37.892341811255996	39.0	37.0	41.0	33.0	41.0
19	37.93267259670449	40.0	37.0	41.0	33.0	41.0
20	37.81284971220555	39.0	37.0	41.0	33.0	41.0
21	37.790396301685576	39.0	37.0	41.0	33.0	41.0
22	37.68857481000291	39.0	37.0	41.0	32.0	41.0
23	37.61241127249648	39.0	37.0	40.0	32.0	41.0
24	37.51547445820737	39.0	36.0	41.0	32.0	41.0
25	37.48225253521182	39.0	36.0	40.0	32.0	41.0
26	37.31648691555168	39.0	36.0	40.0	32.0	41.0
27	37.10990173406188	39.0	36.0	40.0	31.0	41.0
28	36.982297428564856	39.0	36.0	40.0	31.0	41.0
29	36.875153829880006	39.0	36.0	40.0	31.0	41.0
30	36.78916257622751	39.0	35.0	40.0	30.0	41.0
31	36.71587417739945	39.0	35.0	40.0	30.0	41.0
32	36.58318205208639	38.0	35.0	40.0	30.0	41.0
33	36.51858051707042	38.0	35.0	40.0	30.0	41.0
34	36.48029406268571	38.0	35.0	40.0	30.0	41.0
35	36.3457938575792	38.0	35.0	40.0	30.0	41.0
36	36.239060471907706	38.0	35.0	40.0	30.0	41.0
37	36.07613828499535	38.0	35.0	40.0	30.0	41.0
38	35.987665831837354	38.0	35.0	40.0	29.0	41.0
39	35.847681903657744	38.0	35.0	40.0	28.0	41.0
40	35.73749818322212	38.0	35.0	40.0	28.0	41.0
41	35.643311367389806	38.0	35.0	40.0	27.0	41.0
42	35.532695829014685	38.0	34.0	40.0	27.0	41.0
43	34.21594263976283	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	14.0
11	20.0
12	37.0
13	18.0
14	12.0
15	11.0
16	17.0
17	43.0
18	108.0
19	267.0
20	629.0
21	1289.0
22	2512.0
23	4421.0
24	7649.0
25	12514.0
26	19216.0
27	29155.0
28	41978.0
29	58393.0
30	78090.0
31	102478.0
32	132386.0
33	171861.0
34	225923.0
35	299789.0
36	412828.0
37	580770.0
38	834964.0
39	546604.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.361002603253304	18.46472027793475	13.146316977375433	25.027960141436505
2	18.149484764598895	21.473781440077754	35.84967124036406	24.527062554959283
3	20.564578807756	23.26056494917792	29.77725601725252	26.397600225813566
4	15.101787260500977	15.670052934874896	33.509773563538964	35.718386241085156
5	15.74143336619901	35.49097334962214	33.29537974445581	15.472213539723043
6	34.96925647067538	33.7017768227964	15.032735671865504	16.29623103466272
7	28.629389096863584	30.295241136228316	21.784836259912314	19.29053350699579
8	26.424816821090445	34.725766147157046	19.805319974567915	19.04409705718459
9	26.472824650491216	15.143369728748748	20.094208701341916	38.289596919418116
10	17.863373814043875	26.54347556482853	32.324869626896955	23.268280994230643
11	34.545799918181864	22.20422435228712	21.40745151096997	21.842524218561042
12	21.487950904629123	25.428464967191378	30.40837238587408	22.67521174230542
13	30.93519588372846	19.680123636294255	25.016961269943174	24.367719210034114
14	22.01396071045976	21.075493223628943	26.917324962219435	29.99322110369186
15	25.152146379266902	27.24036069564495	23.755065232847794	23.85242769224035
16	23.61294971214943	26.025714912617893	25.898330023383824	24.46300535184885
17	23.493056401202917	26.382925711040567	26.51572586098436	23.60829202677215
18	23.64690031038142	24.877567408772496	27.570130432025568	23.905401848820514
19	24.620019854085378	25.103689616335796	27.979838395152417	22.29645213442641
20	24.02338719226308	25.096310271430823	27.6192044785609	23.261098057745198
21	23.30456043515127	25.87725820580348	27.936095434289882	22.882085924755373
22	23.809582598438496	25.525995776657812	27.18634838027588	23.478073244627808
23	23.239184489795463	25.51227524563679	27.50966469715786	23.738875567409895
24	23.22933601047362	25.65980602704488	27.52206648593351	23.588791476547993
25	23.686602869470892	25.3154459509282	27.313789386201243	23.684161793399667
26	23.20436408284844	25.745524272994235	27.761432232641848	23.28867941151548
27	23.661687058537005	25.94600115263684	27.494036198632887	22.898275590193272
28	23.486771331778154	26.008487088391085	27.34358734927758	23.161154230553183
29	23.257450472811183	26.164996540405983	27.708373901024746	22.86917908575809
30	23.266260793344113	26.068672239802332	27.686460333075008	22.978606633778544
31	23.08960544915519	25.584862185823688	27.85068583014263	23.474846534878488
32	22.660733635951942	26.050714898588723	27.66841881682446	23.62013264863488
33	22.96598037823772	25.706270647435105	27.572739858170674	23.7550091161565
34	23.222293365716407	25.738341336508793	27.787358144019	23.252007153755805
35	22.924145384879132	25.763874431046897	27.843923768841883	23.46805641523209
36	22.67697941808113	25.47838076409609	28.073946086450007	23.770693731372766
37	22.45876966399009	25.007786190916843	28.345943689144953	24.187500455948115
38	22.422798864871567	24.964969155460633	28.68253160351762	23.92970037615018
39	21.99325365137281	24.92930699814422	29.144119447744416	23.93331990273855
40	22.290335415075525	24.901445060917474	29.196139620572602	23.612079903434395
41	21.807675753268377	24.903128561656253	29.395522224735004	23.89367346034037
42	21.38918552795425	25.250266413991913	29.26622936799699	24.094318690056852
43	21.24923049987065	24.92930699814422	29.13197018407958	24.689492317905547
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3232.0
1	2805.5
2	2379.0
3	5137.5
4	7896.0
5	7896.0
6	9630.0
7	11364.0
8	10689.0
9	10014.0
10	14107.5
11	18201.0
12	18201.0
13	28000.5
14	37800.0
15	47411.0
16	57022.0
17	50965.5
18	44909.0
19	44909.0
20	46372.0
21	47835.0
22	38218.5
23	28602.0
24	31860.0
25	35118.0
26	35118.0
27	40525.0
28	45932.0
29	52111.5
30	58291.0
31	66215.0
32	74139.0
33	74139.0
34	83921.5
35	93704.0
36	107125.0
37	120546.0
38	136319.0
39	152092.0
40	152092.0
41	170384.0
42	188676.0
43	207869.0
44	227062.0
45	246157.0
46	265252.0
47	265252.0
48	282541.0
49	299830.0
50	306667.0
51	313504.0
52	311750.0
53	309996.0
54	309996.0
55	291236.0
56	272476.0
57	250694.5
58	228913.0
59	209310.5
60	189708.0
61	189708.0
62	166363.5
63	143019.0
64	121102.5
65	99186.0
66	82399.0
67	65612.0
68	65612.0
69	53710.0
70	41808.0
71	34524.0
72	27240.0
73	22155.0
74	17070.0
75	17070.0
76	13902.0
77	10734.0
78	8737.0
79	6740.0
80	5373.0
81	4006.0
82	4006.0
83	3148.0
84	2290.0
85	1747.0
86	1204.0
87	823.0
88	442.0
89	442.0
90	279.0
91	116.0
92	72.0
93	28.0
94	18.0
95	8.0
96	8.0
97	6.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3564002.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.03168372151366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.68904861384921	47.98584716391765
2	9.057504402222392	10.512444615519751
3	2.9203093535414952	5.084114063210943
4	1.4553138891519375	3.3781726132316496
5	0.8606234128335855	2.497171284844416
6	0.5881444457083981	2.0478607473548833
7	0.41590011615734435	1.689476880040866
8	0.2958320623833053	1.3734106143128857
9	0.23197335717014136	1.2115624045603832
>10	1.3818492634302066	14.503687868981356
>50	0.07138089223208235	2.80905863649099
>100	0.029462788910267792	3.1246435118719553
>500	0.0016427578598009961	0.6294435781481705
>1k	8.213789213013711E-4	1.0457079625022312
>5k	0.0	0.0
>10k+	1.932656285414991E-4	2.10739805501187
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	24039	0.6744945709907009	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20602	0.5780580370044686	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	17968	0.5041523545721915	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12551	0.3521602962063433	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4359	0.1223063286720939	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4261	0.11955661079875939	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4068	0.11414135008902912	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4025	0.11293484122623948	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.805834564627068E-5	0.0
5	2.805834564627068E-5	0.0	0.0	5.611669129254136E-5	0.0
6	2.805834564627068E-5	0.0	0.0	5.611669129254136E-5	2.805834564627068E-5
7	2.805834564627068E-5	0.0	0.0	5.611669129254136E-5	5.611669129254136E-5
8	8.417503693881205E-5	0.0	0.0	5.611669129254136E-5	8.417503693881205E-5
9	1.1223338258508272E-4	0.0	0.0	8.417503693881205E-5	1.9640841952389476E-4
10	1.402917282313534E-4	0.0	0.0	8.417503693881205E-5	1.9640841952389476E-4
11	1.9640841952389476E-4	0.0	0.0	1.683500738776241E-4	1.9640841952389476E-4
12	1.9640841952389476E-4	0.0	0.0	3.928168390477895E-4	1.9640841952389476E-4
13	1.9640841952389476E-4	0.0	2.805834564627068E-5	4.489335303403309E-4	1.9640841952389476E-4
14	1.9640841952389476E-4	0.0	2.805834564627068E-5	6.17283604217955E-4	2.2446676517016544E-4
15	2.2446676517016544E-4	0.0	2.805834564627068E-5	7.014586411567671E-4	3.367001477552482E-4
16	2.2446676517016544E-4	0.0	2.805834564627068E-5	0.001571267356191158	4.208751846940602E-4
17	2.2446676517016544E-4	0.0	2.805834564627068E-5	0.00246913441687182	4.208751846940602E-4
18	2.2446676517016544E-4	0.0	2.805834564627068E-5	0.0028619512559196095	5.050502216328723E-4
19	2.2446676517016544E-4	0.0	5.611669129254136E-5	0.00370370162530773	5.050502216328723E-4
20	2.5252511081643613E-4	0.0	5.611669129254136E-5	0.005050502216328722	5.33108567279143E-4
21	3.086418021089775E-4	0.0	5.611669129254136E-5	0.008810320532928994	5.33108567279143E-4
22	3.086418021089775E-4	0.0	5.611669129254136E-5	0.013720531021026362	5.33108567279143E-4
23	3.086418021089775E-4	0.0	5.611669129254136E-5	0.01801345790490578	7.295169868030378E-4
24	3.086418021089775E-4	0.0	5.611669129254136E-5	0.024747460860010742	7.295169868030378E-4
25	3.086418021089775E-4	0.0	5.611669129254136E-5	0.02962961300246184	7.295169868030378E-4
26	3.086418021089775E-4	0.0	5.611669129254136E-5	0.039730617435119284	7.575753324493083E-4
27	3.086418021089775E-4	0.0	5.611669129254136E-5	0.07146460636105142	7.575753324493083E-4
28	3.086418021089775E-4	0.0	5.611669129254136E-5	0.16554423931299703	7.575753324493083E-4
29	3.086418021089775E-4	0.0	5.611669129254136E-5	0.31921979841762155	7.575753324493083E-4
30	3.086418021089775E-4	0.0	5.611669129254136E-5	0.5008134114402853	7.575753324493083E-4
31	3.086418021089775E-4	0.0	5.611669129254136E-5	0.918826644878426	7.85633678095579E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3540	0.0	15.1031065	37
TCGTTAC	330	0.0	14.575757	23
GTACTAA	165	1.6483682E-8	14.575757	1
CGTACGA	80	0.0063020443	13.875001	10
CGTATAC	95	0.0012459721	13.631579	3
GTCCTAC	290	0.0	13.396551	1
GTCTTAA	430	0.0	12.906978	1
CGTTACA	245	1.1459633E-10	12.836734	24
CGTTACG	205	2.0696461E-8	12.634146	24
TATACTG	500	0.0	12.580001	5
TCTTATA	5245	0.0	12.345091	37
ATACCCG	500	0.0	12.210001	5
GTCTTAG	470	0.0	12.2021265	1
ACCCGTT	535	0.0	12.102803	7
ATACCGT	220	5.5342753E-8	11.772727	6
TACCCGT	535	0.0	11.7570095	6
ACCGTGC	425	0.0	11.75294	8
CTCTTAT	7735	0.0	11.623788	37
GTCTAGA	385	0.0	11.532467	1
GGCTAGT	305	3.092282E-11	11.52459	1
>>END_MODULE