##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727071.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4405855
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61878818072769	31.0	31.0	34.0	30.0	34.0
2	31.844746592886057	31.0	31.0	34.0	30.0	34.0
3	31.95148115405523	33.0	31.0	34.0	30.0	34.0
4	35.6851097006143	37.0	35.0	37.0	33.0	37.0
5	35.29546069945561	37.0	35.0	37.0	33.0	37.0
6	35.328487206228985	37.0	35.0	37.0	33.0	37.0
7	35.266486527586586	37.0	35.0	37.0	32.0	37.0
8	35.227073746185475	37.0	35.0	37.0	32.0	37.0
9	37.05292230452432	39.0	37.0	39.0	33.0	39.0
10	36.829899758389686	39.0	37.0	39.0	33.0	39.0
11	36.94880743919171	39.0	37.0	39.0	33.0	39.0
12	36.83409077239265	39.0	37.0	39.0	33.0	39.0
13	36.967934033235316	39.0	37.0	39.0	33.0	39.0
14	37.96765871777442	40.0	37.0	41.0	33.0	41.0
15	37.97914547800597	40.0	37.0	41.0	33.0	41.0
16	37.91901639976803	39.0	37.0	41.0	33.0	41.0
17	37.85173388593133	39.0	37.0	41.0	33.0	41.0
18	37.896763511282145	39.0	37.0	41.0	33.0	41.0
19	37.9417227757155	40.0	37.0	41.0	33.0	41.0
20	37.82261467978406	39.0	37.0	41.0	33.0	41.0
21	37.80048458244767	39.0	37.0	41.0	33.0	41.0
22	37.69721563691951	39.0	37.0	41.0	32.0	41.0
23	37.62320457663723	39.0	37.0	40.0	32.0	41.0
24	37.52133740216144	39.0	36.0	41.0	32.0	41.0
25	37.491491435828	39.0	36.0	40.0	32.0	41.0
26	37.32672500570264	39.0	36.0	40.0	32.0	41.0
27	37.120101092750446	39.0	36.0	40.0	31.0	41.0
28	36.99068534938168	39.0	36.0	40.0	31.0	41.0
29	36.87482679298343	39.0	36.0	40.0	31.0	41.0
30	36.779978006539025	39.0	35.0	40.0	31.0	41.0
31	36.705425167192296	39.0	35.0	40.0	30.0	41.0
32	36.564854948698944	38.0	35.0	40.0	30.0	41.0
33	36.49570128839919	38.0	35.0	40.0	30.0	41.0
34	36.445117009070884	38.0	35.0	40.0	30.0	41.0
35	36.31477976465408	38.0	35.0	40.0	30.0	41.0
36	36.1917264639894	38.0	35.0	40.0	30.0	41.0
37	36.0221936491328	38.0	35.0	40.0	29.0	41.0
38	35.92007703385608	38.0	35.0	40.0	29.0	41.0
39	35.78087658354621	38.0	35.0	40.0	28.0	41.0
40	35.66166930141823	38.0	35.0	40.0	27.0	41.0
41	35.55817520095418	38.0	34.0	40.0	27.0	41.0
42	35.42930736485881	38.0	34.0	40.0	27.0	41.0
43	34.10965817077503	37.0	33.0	40.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	18.0
11	22.0
12	20.0
13	24.0
14	12.0
15	12.0
16	26.0
17	52.0
18	131.0
19	345.0
20	787.0
21	1634.0
22	2988.0
23	5558.0
24	9438.0
25	15397.0
26	23809.0
27	35994.0
28	51965.0
29	72746.0
30	97298.0
31	126801.0
32	164270.0
33	214431.0
34	280389.0
35	374591.0
36	513957.0
37	721684.0
38	1025830.0
39	665621.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.76462684314395	18.358389007354987	13.091193423296954	24.78579072620411
2	18.070976008061997	21.656069026329735	35.79557202858469	24.477382937023574
3	20.672378006085086	23.565528143799558	29.740583836735436	26.021510013379924
4	15.056351150911684	15.628498895220112	33.423274256642586	35.89187569722562
5	15.758099165769188	35.55321271353687	33.21160138043581	15.477086740258134
6	35.0010837850996	33.58369714845359	14.929837681902832	16.485381384543977
7	28.46353318481884	30.346345941934082	21.95403616324187	19.23608471000521
8	26.56882716294567	34.55388341195977	19.938831395949254	18.93845802914531
9	26.40266191238704	15.188743161089052	20.180123948700082	38.228470977823825
10	17.91280012619571	26.50495760754723	32.30623794927432	23.276004316982743
11	34.227976181694586	22.29233599380824	21.40758150234177	22.072106322155406
12	21.455131864303294	25.65141158753522	30.433525388375244	22.45993115978624
13	31.038447701978388	19.602755878257454	25.125520472189848	24.233275947574306
14	21.972080334010087	21.10804826758938	27.203550729654065	29.716320668746476
15	25.239368976055726	27.142177851971976	24.048567190704187	23.569885981268108
16	23.45989597932751	25.874977728499914	26.2602151001338	24.404911192038774
17	23.358508166973266	26.448714267718753	26.804717813001112	23.388059752306873
18	23.4504313010755	24.79900042103065	27.89326929733275	23.857298980561094
19	24.377152675246915	25.1865528938197	28.277303724248753	22.158990706684627
20	23.733668039461126	25.200398106610404	27.947901145180676	23.11803270874779
21	23.021070825072545	26.03049805315881	28.215159146181616	22.733271975587034
22	23.481889440301597	25.733870043385448	27.447181080630205	23.337059435682743
23	23.044267230764518	25.599616873455886	27.73341383227546	23.622702063504132
24	22.971023785394664	25.806432576650845	27.831283598756656	23.39126003919784
25	23.412254829085388	25.411163100011237	27.559576972006568	23.617005098896808
26	22.921544172470494	25.83446345828449	28.14112130335656	23.102871065888458
27	23.509035136199444	26.035627591012418	27.864217047542418	22.591120225245724
28	23.30941440424163	26.15226783450658	27.677942192832038	22.860375568419748
29	23.02447538559485	26.272698488715584	28.085626966842987	22.617199158846578
30	22.912737709252802	26.27555831955432	28.019306127868482	22.792397843324395
31	22.790854442554284	25.703251695754854	28.179819807960087	23.326074053730775
32	22.343404401642815	26.16699823303309	28.0086385048986	23.480958860425503
33	22.775170767081534	25.87981220444159	27.841474583253422	23.50354244522346
34	23.025110903559014	25.902214212678352	28.02656919031607	23.046105693446563
35	22.673624075236248	25.99543107977907	28.14089433265507	23.190050512329616
36	22.451238181919287	25.695784359675933	28.281956623629238	23.571020834775545
37	22.12875820924656	25.20645822434011	28.55554710720167	24.109236459211665
38	22.147505989189387	25.201691839608888	28.932000712688005	23.718801458513727
39	21.768828070828476	25.051391841084193	29.372505450133968	23.80727463795336
40	22.02902728301317	25.202803996046168	29.42577547377297	23.34239324716769
41	21.6199806847933	25.096082372206986	29.57462285980814	23.709314083191572
42	21.173211555986295	25.448862933528225	29.492119009817618	23.88580650066786
43	21.05005725335945	25.117009070884084	29.34651730481371	24.486416370942756
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4106.0
1	3489.5
2	2873.0
3	6567.5
4	10262.0
5	10262.0
6	12703.0
7	15144.0
8	14237.5
9	13331.0
10	18526.0
11	23721.0
12	23721.0
13	37027.0
14	50333.0
15	64380.0
16	78427.0
17	69995.5
18	61564.0
19	61564.0
20	63320.5
21	65077.0
22	51336.5
23	37596.0
24	41915.5
25	46235.0
26	46235.0
27	52705.5
28	59176.0
29	66454.5
30	73733.0
31	83266.5
32	92800.0
33	92800.0
34	104641.0
35	116482.0
36	132723.5
37	148965.0
38	167716.5
39	186468.0
40	186468.0
41	207707.0
42	228946.0
43	252247.5
44	275549.0
45	300088.5
46	324628.0
47	324628.0
48	346413.5
49	368199.0
50	375539.5
51	382880.0
52	384170.0
53	385460.0
54	385460.0
55	360736.5
56	336013.0
57	306998.5
58	277984.0
59	254039.0
60	230094.0
61	230094.0
62	201586.5
63	173079.0
64	146910.5
65	120742.0
66	100365.0
67	79988.0
68	79988.0
69	65740.0
70	51492.0
71	42321.5
72	33151.0
73	27025.5
74	20900.0
75	20900.0
76	16891.5
77	12883.0
78	10478.0
79	8073.0
80	6317.5
81	4562.0
82	4562.0
83	3668.5
84	2775.0
85	2096.5
86	1418.0
87	987.5
88	557.0
89	557.0
90	348.5
91	140.0
92	84.5
93	29.0
94	20.5
95	12.0
96	12.0
97	9.5
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4405855.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.15547855662053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.17622556085306	45.32469046785643
2	9.091148682103668	10.028533123816356
3	3.0907873055047452	5.114215588555257
4	1.4948973443647857	3.298071136858437
5	0.90560682488622	2.4974588905370556
6	0.5897146149867565	1.9515595080856663
7	0.4390410065359114	1.6950861775027988
8	0.31227517498757784	1.3778949374233818
9	0.24857582819845292	1.2339286885704561
>10	1.5191794085329853	15.43006587183953
>50	0.09262792647995521	3.4502576037682084
>100	0.035560067072035795	3.4886462961463867
>500	0.0029205487494245457	1.1026557488553845
>1k	0.0011106309171589914	1.1615306714622016
>5k	1.6453791365317674E-4	0.5052599432272558
>10k+	1.6453791365317674E-4	2.340145345495074
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	33163	0.7527029373413332	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28332	0.6430533914529643	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	24876	0.5646123170190577	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16774	0.38072065467429134	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6046	0.13722648611903934	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5861	0.13302752814152985	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5203	0.11809285598368535	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5160	0.11711688196729124	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	4436	0.10068420317963256	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2697070148699854E-5
2	0.0	0.0	0.0	0.0	2.2697070148699854E-5
3	0.0	0.0	0.0	0.0	2.2697070148699854E-5
4	0.0	0.0	0.0	2.2697070148699854E-5	2.2697070148699854E-5
5	0.0	0.0	0.0	4.539414029739971E-5	2.2697070148699854E-5
6	0.0	0.0	0.0	4.539414029739971E-5	4.539414029739971E-5
7	0.0	0.0	0.0	4.539414029739971E-5	4.539414029739971E-5
8	0.0	0.0	0.0	4.539414029739971E-5	4.539414029739971E-5
9	0.0	0.0	0.0	4.539414029739971E-5	1.5887949104089898E-4
10	2.2697070148699854E-5	0.0	0.0	1.1348535074349927E-4	1.5887949104089898E-4
11	4.539414029739971E-5	0.0	0.0	2.0427363133829868E-4	1.5887949104089898E-4
12	4.539414029739971E-5	0.0	0.0	2.950619119330981E-4	1.5887949104089898E-4
13	4.539414029739971E-5	0.0	0.0	3.858501925278975E-4	1.5887949104089898E-4
14	1.1348535074349927E-4	0.0	0.0	6.128208940148961E-4	2.2697070148699854E-4
15	1.5887949104089898E-4	0.0	0.0	7.943974552044949E-4	2.496677716356984E-4
16	1.5887949104089898E-4	0.0	0.0	0.0016114919805576898	3.1775898208179795E-4
17	1.8157656118959883E-4	0.0	0.0	0.002610163067100483	3.1775898208179795E-4
18	2.0427363133829868E-4	0.0	0.0	0.002996013259628381	3.1775898208179795E-4
19	2.0427363133829868E-4	0.0	0.0	0.004584808170037371	3.1775898208179795E-4
20	2.2697070148699854E-4	0.0	0.0	0.006377876711784659	3.1775898208179795E-4
21	2.7236484178439825E-4	0.0	0.0	0.011620899916134325	3.1775898208179795E-4
22	2.7236484178439825E-4	0.0	0.0	0.01740865280405279	3.1775898208179795E-4
23	2.7236484178439825E-4	0.0	0.0	0.022515493587510257	4.312443328252972E-4
24	2.7236484178439825E-4	2.2697070148699854E-5	0.0	0.030822621261934403	4.312443328252972E-4
25	2.7236484178439825E-4	2.2697070148699854E-5	0.0	0.03735937746475996	4.312443328252972E-4
26	2.7236484178439825E-4	2.2697070148699854E-5	0.0	0.04839015355702809	4.539414029739971E-4
27	2.7236484178439825E-4	2.2697070148699854E-5	0.0	0.08227687928903697	4.7663847312269693E-4
28	2.7236484178439825E-4	2.2697070148699854E-5	0.0	0.17490362256588107	4.7663847312269693E-4
29	2.7236484178439825E-4	2.2697070148699854E-5	0.0	0.3272236603338058	4.7663847312269693E-4
30	2.7236484178439825E-4	2.2697070148699854E-5	0.0	0.5084597654711741	4.7663847312269693E-4
31	2.7236484178439825E-4	2.2697070148699854E-5	0.0	0.9365265084756534	4.7663847312269693E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4600	0.0	15.363043	37
AAACCGT	105	1.6573122E-4	14.095238	6
CGGACTA	80	0.006302357	13.875	29
ATACCCG	585	0.0	13.282051	5
TAAGGCG	330	0.0	12.893938	5
TATACAC	1910	0.0	12.688481	37
TCGTTAC	500	0.0	12.58	23
TCTTATA	6825	0.0	12.550183	37
CGCGTAT	120	5.1265553E-4	12.333333	7
TATACTG	585	0.0	12.333333	5
TACCCGT	620	0.0	11.935483	6
TAGAGTG	460	0.0	11.663044	5
ACCGTGC	445	0.0	11.6404505	8
CGGTTAA	175	7.3452557E-6	11.6285715	21
GTAAGAG	1385	0.0	11.620938	3
ACCCGTT	680	0.0	11.426471	7
ACTAGAC	390	0.0	11.384616	3
AATACCG	185	1.361654E-5	11.000001	5
TAGGACT	650	0.0	10.815385	4
AACCGTC	240	1.845874E-7	10.791666	7
>>END_MODULE