FastQCFastQC Report
Thu 9 Feb 2017
SRR2727070.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727070.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6063138
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT830211.3692744582095937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT646521.0663125266157556No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT592100.9765570237721786No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405960.6695542803083157No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135640.22371253961232618No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125410.20684008841626236No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114010.18803794338839064No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112720.18591033224049988No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT101160.16684429745785104No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT81110.13377561256233983No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG67030.11055331414195092No Hit
GTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA62260.10268610082765724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCCG25600.015.3925795
CTCCATA27650.014.9873411
TCCATAC27250.014.867892
ACCCGTT26600.014.813917
TATACCG2151.2732926E-1114.6279075
TACCCGT27100.014.2675286
CATACCC28950.014.1226254
TATACAC42550.013.56521737
CCATACC29750.013.4941183
CTTATAC91050.013.38989537
CCGTTTT28650.013.3019219
CCCGTTT29000.013.2689668
TCGTTAC4500.013.15555623
TACACCG3950.012.1772155
GTATTAG4850.011.8247421
GTATTAC4000.011.5625011
CGTTTTT32650.011.50229610
TAACGCT1951.9640738E-611.3846164
TCTACAC12200.011.0696723
CTCGTTA6000.010.79166722
Produced by FastQC (version 0.11.5)