##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727070.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6063138
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.958748753533236	33.0	31.0	34.0	30.0	34.0
2	32.1347882894963	33.0	31.0	34.0	30.0	34.0
3	32.22061199992479	34.0	31.0	34.0	30.0	34.0
4	35.85219056534751	37.0	35.0	37.0	35.0	37.0
5	35.57851116039252	37.0	35.0	37.0	33.0	37.0
6	35.638492641928984	37.0	35.0	37.0	33.0	37.0
7	35.58437940881438	37.0	35.0	37.0	33.0	37.0
8	35.577769960043796	37.0	35.0	37.0	33.0	37.0
9	37.378424505594296	39.0	37.0	39.0	34.0	39.0
10	37.2194241661661	39.0	37.0	39.0	34.0	39.0
11	37.307803154076325	39.0	37.0	39.0	34.0	39.0
12	37.22456886186658	39.0	37.0	39.0	34.0	39.0
13	37.30830718350795	39.0	37.0	39.0	34.0	39.0
14	38.38157419474866	40.0	38.0	41.0	34.0	41.0
15	38.34291104705187	40.0	38.0	41.0	34.0	41.0
16	38.28465573437385	40.0	38.0	41.0	34.0	41.0
17	38.30607269700937	40.0	38.0	41.0	34.0	41.0
18	38.36348867533611	40.0	38.0	41.0	34.0	41.0
19	38.42299367093409	40.0	38.0	41.0	34.0	41.0
20	38.34955133793755	40.0	38.0	41.0	34.0	41.0
21	38.262655740311374	40.0	38.0	41.0	34.0	41.0
22	38.17592226995328	40.0	38.0	41.0	34.0	41.0
23	38.091225368777685	40.0	37.0	41.0	33.0	41.0
24	37.99715691775447	40.0	37.0	41.0	33.0	41.0
25	37.95400352094905	40.0	37.0	41.0	33.0	41.0
26	37.733557936500866	39.0	37.0	41.0	33.0	41.0
27	37.55559019108587	39.0	36.0	41.0	33.0	41.0
28	37.425169112100036	39.0	36.0	41.0	32.0	41.0
29	37.27116882380048	39.0	36.0	41.0	32.0	41.0
30	37.12702333346198	39.0	36.0	40.0	32.0	41.0
31	36.97441984662068	39.0	35.0	40.0	31.0	41.0
32	36.79610046810744	39.0	35.0	40.0	31.0	41.0
33	36.62489093931228	39.0	35.0	40.0	31.0	41.0
34	36.49876911922506	39.0	35.0	40.0	30.0	41.0
35	36.33349298663497	39.0	35.0	40.0	30.0	41.0
36	36.11117840299858	38.0	35.0	40.0	30.0	41.0
37	35.94850240914853	38.0	35.0	40.0	29.0	41.0
38	35.80460398559294	38.0	35.0	40.0	28.0	41.0
39	35.596176435370595	38.0	35.0	40.0	27.0	41.0
40	35.385594720093785	38.0	35.0	40.0	25.0	41.0
41	35.20347664856053	38.0	35.0	40.0	24.0	41.0
42	34.90279868279429	38.0	34.0	40.0	23.0	41.0
43	33.67544561908372	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	25.0
10	48.0
11	35.0
12	21.0
13	15.0
14	16.0
15	22.0
16	44.0
17	108.0
18	260.0
19	555.0
20	1158.0
21	2517.0
22	4578.0
23	8062.0
24	13638.0
25	22287.0
26	33886.0
27	50524.0
28	71562.0
29	97105.0
30	124295.0
31	157056.0
32	197843.0
33	253977.0
34	331929.0
35	443967.0
36	632163.0
37	962119.0
38	1419354.0
39	1233965.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.72770733570636	17.964707384196103	13.569128725092519	22.738456555005016
2	17.91583830023331	23.092745043903008	35.73190978005118	23.25950687581249
3	21.57391106717347	24.813223779501637	30.009246037283006	23.60361911604189
4	14.97514983165483	15.975720823111727	33.000584845669025	36.048544499564414
5	15.774686309300565	36.05115041089284	32.57817981381918	15.595983465987414
6	34.25003686210012	33.251576988681435	14.67198998274491	17.826396166473533
7	27.42319241290566	30.910413056737283	22.50860857859412	19.157785951762932
8	26.982727425963255	34.05114645254652	20.129609453058794	18.83651666843143
9	26.156966903936542	15.470652325577944	20.77168621265094	37.60069455783457
10	18.652321619596982	27.160836517328157	31.164736807903765	23.022105055171103
11	32.49352068186474	23.280832466620417	21.204102562072645	23.0215442894422
12	21.487586131141992	27.214208220231832	30.404701327926233	20.89350432069994
13	31.384408535646063	19.734302600402632	25.89545215695239	22.985836706998917
14	21.451532193395565	22.32088400428953	29.045471173507842	27.182112628807065
15	25.107675266503914	27.407886807128584	25.615679537559593	21.86875838880791
16	21.964814259546788	26.035412685642317	28.081349954429534	23.918423100381354
17	22.252487078473226	27.192651725888478	28.292296827154516	22.26256436848378
18	22.546493251514317	24.598895819293574	29.612207408111114	23.242403521081
19	23.781431331432668	25.381691790620632	30.43328388699053	20.403592990956167
20	23.046300447062233	25.830172428864394	29.555438124614682	21.568088999458695
21	21.649713399233203	27.228210870344693	29.991680875480654	21.13039485494145
22	21.901480718400272	27.191761097966104	29.213008181571986	21.693750002061638
23	21.525041983210674	26.724131959391322	29.479371902800168	22.271454154597834
24	21.590668066601815	27.025444580017805	29.669191102033306	21.714696251347075
25	21.868923319904642	26.449554669545705	29.392090366407626	22.289431644142027
26	21.240832717315687	27.14424444899654	30.37920298037089	21.23571985331688
27	22.06151336156294	27.132435382470266	30.167266521065493	20.6387847349013
28	21.950630185227517	27.54098620219431	29.68774585041607	20.8206377621621
29	21.6739912566727	27.58657315733206	30.00964187191517	20.72979371408007
30	20.984166944575563	27.761152723226818	30.317601215740098	20.937079116457518
31	20.736456930388194	27.30978579079018	30.324495335583652	21.629261943237974
32	20.53192587732623	27.514019967878017	30.017970892300323	21.936083262495426
33	20.9875150458393	27.55046974025661	29.660334302138597	21.80168091176549
34	21.22506530446775	27.7760789874814	29.48714015745642	21.511715550594428
35	20.583912159017327	27.82221021523838	29.924471453560848	21.669406172183447
36	20.383999176663963	27.644117616983156	30.018795547784	21.95308765856888
37	20.030172494836833	26.923847024428603	30.089122167432116	22.956858313302455
38	20.112654536314363	27.0289411192686	30.467589555111562	22.390814789305473
39	19.597871597182845	26.830430051237496	30.85234081757664	22.71935753400302
40	19.949075874571882	27.0890420109191	30.85461686671159	22.10726524779743
41	19.794865299123984	26.913901679295442	30.988244041286872	22.302988980293705
42	19.116503698249982	27.365631460144897	30.817721780371816	22.70014306123331
43	19.225638604960004	26.80963223993912	30.567587279062426	23.397141876038447
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5735.0
1	5259.0
2	4783.0
3	12402.5
4	20022.0
5	20022.0
6	26615.5
7	33209.0
8	31898.0
9	30587.0
10	43724.0
11	56861.0
12	56861.0
13	88785.0
14	120709.0
15	157186.0
16	193663.0
17	169988.5
18	146314.0
19	146314.0
20	145830.5
21	145347.0
22	107587.5
23	69828.0
24	74204.0
25	78580.0
26	78580.0
27	86219.0
28	93858.0
29	102441.0
30	111024.0
31	119117.0
32	127210.0
33	127210.0
34	138098.0
35	148986.0
36	167354.0
37	185722.0
38	210403.0
39	235084.0
40	235084.0
41	265362.5
42	295641.0
43	331682.5
44	367724.0
45	409123.5
46	450523.0
47	450523.0
48	474279.5
49	498036.0
50	499714.5
51	501393.0
52	503408.0
53	505423.0
54	505423.0
55	465925.0
56	426427.0
57	380310.0
58	334193.0
59	303038.5
60	271884.0
61	271884.0
62	238373.5
63	204863.0
64	173870.0
65	142877.0
66	118540.0
67	94203.0
68	94203.0
69	77283.0
70	60363.0
71	49901.5
72	39440.0
73	32317.0
74	25194.0
75	25194.0
76	20359.5
77	15525.0
78	12729.0
79	9933.0
80	7814.5
81	5696.0
82	5696.0
83	4470.5
84	3245.0
85	2516.0
86	1787.0
87	1309.0
88	831.0
89	831.0
90	549.5
91	268.0
92	175.0
93	82.0
94	62.5
95	43.0
96	43.0
97	30.0
98	17.0
99	11.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6063138.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.43350704821073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.8432630452983	38.346794874595105
2	9.675968872647601	9.179302754379954
3	3.2853252448142993	4.675034944666912
4	1.5752270384926215	2.988741713314875
5	0.9669700443616298	2.2933390207318003
6	0.6339621080286555	1.8042627671685456
7	0.48776270713884307	1.6195407064847258
8	0.3498331337197022	1.3275049931192913
9	0.2669176121407112	1.1394754593091203
>10	1.7110891332097447	15.208717687606882
>50	0.13007945292365816	4.209807012283993
>100	0.06258429092688697	5.495040900872252
>500	0.006394921364255385	2.091795927048519
>1k	0.0041010721961577965	3.948840782240358
>5k	2.0852909471437994E-4	0.621105580853163
>10k+	3.127936420715699E-4	5.050694875324506
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	83021	1.3692744582095937	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	64652	1.0663125266157556	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	59210	0.9765570237721786	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40596	0.6695542803083157	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13564	0.22371253961232618	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12541	0.20684008841626236	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11401	0.18803794338839064	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11272	0.18591033224049988	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10116	0.16684429745785104	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	8111	0.13377561256233983	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	6703	0.11055331414195092	No Hit
GTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA	6226	0.10268610082765724	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2986219347143346E-5	0.0	0.0	0.0	3.2986219347143346E-5
2	9.895865804143003E-5	0.0	0.0	0.0	3.2986219347143346E-5
3	9.895865804143003E-5	0.0	0.0	0.0	3.2986219347143346E-5
4	9.895865804143003E-5	0.0	0.0	0.0	4.9479329020715016E-5
5	1.3194487738857338E-4	0.0	0.0	0.0	4.9479329020715016E-5
6	1.9791731608286006E-4	0.0	0.0	0.0	8.246554836785836E-5
7	2.3090353543000342E-4	0.0	0.0	0.0	8.246554836785836E-5
8	2.8038286445071845E-4	0.0	0.0	3.2986219347143346E-5	1.3194487738857338E-4
9	3.298621934714334E-4	0.0	0.0	6.597243869428669E-5	1.4843798706214506E-4
10	4.947932902071502E-4	0.0	0.0	2.1441042575643174E-4	1.4843798706214506E-4
11	5.772588385750086E-4	0.0	0.0	5.442726192278652E-4	1.4843798706214506E-4
12	6.597243869428668E-4	0.0	0.0	0.001203997006170732	1.4843798706214506E-4
13	6.597243869428668E-4	0.0	0.0	0.0015008729802950221	1.649310967357167E-4
14	7.421899353107252E-4	1.6493109673571673E-5	0.0	0.0023420215736471776	1.814242064092884E-4
15	7.421899353107252E-4	1.6493109673571673E-5	0.0	0.0035625116894914812	2.3090353543000342E-4
16	7.421899353107252E-4	3.2986219347143346E-5	0.0	0.006877626733879387	2.6388975477714677E-4
17	7.421899353107252E-4	3.2986219347143346E-5	0.0	0.009796907146101572	2.6388975477714677E-4
18	8.57641703025727E-4	3.2986219347143346E-5	0.0	0.011924518293992319	3.298621934714334E-4
19	9.236141417200137E-4	3.2986219347143346E-5	0.0	0.016822971867043107	3.298621934714334E-4
20	9.56600361067157E-4	3.2986219347143346E-5	0.0	0.023205805310715342	3.463553031450051E-4
21	0.0010225727997614437	3.2986219347143346E-5	0.0	0.040391625590577025	3.628484128185768E-4
22	0.0011215314578028738	3.2986219347143346E-5	0.0	0.06165124395981091	3.628484128185768E-4
23	0.0011215314578028738	3.2986219347143346E-5	0.0	0.07817733985272972	4.947932902071502E-4
24	0.0011875038964971605	3.2986219347143346E-5	0.0	0.1054404501431437	5.112863998807219E-4
25	0.001203997006170732	3.2986219347143346E-5	0.0	0.12374780188080825	5.442726192278652E-4
26	0.0012369832255178753	3.2986219347143346E-5	0.0	0.15122532259697866	5.772588385750086E-4
27	0.0012699694448650187	3.2986219347143346E-5	0.0	0.19945117528250222	5.772588385750086E-4
28	0.001302955664212162	3.2986219347143346E-5	0.0	0.33358963625766064	5.772588385750086E-4
29	0.001302955664212162	3.2986219347143346E-5	0.0	0.5801946120969043	5.937519482485802E-4
30	0.001302955664212162	3.2986219347143346E-5	0.0	0.862721580805187	5.937519482485802E-4
31	0.001302955664212162	3.2986219347143346E-5	0.0	1.434339775871834	5.937519482485802E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCCG	2560	0.0	15.392579	5
CTCCATA	2765	0.0	14.987341	1
TCCATAC	2725	0.0	14.86789	2
ACCCGTT	2660	0.0	14.81391	7
TATACCG	215	1.2732926E-11	14.627907	5
TACCCGT	2710	0.0	14.267528	6
CATACCC	2895	0.0	14.122625	4
TATACAC	4255	0.0	13.565217	37
CCATACC	2975	0.0	13.494118	3
CTTATAC	9105	0.0	13.389895	37
CCGTTTT	2865	0.0	13.301921	9
CCCGTTT	2900	0.0	13.268966	8
TCGTTAC	450	0.0	13.155556	23
TACACCG	395	0.0	12.177215	5
GTATTAG	485	0.0	11.824742	1
GTATTAC	400	0.0	11.562501	1
CGTTTTT	3265	0.0	11.502296	10
TAACGCT	195	1.9640738E-6	11.384616	4
TCTACAC	1220	0.0	11.069672	3
CTCGTTA	600	0.0	10.791667	22
>>END_MODULE