FastQCFastQC Report
Thu 9 Feb 2017
SRR2727069.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727069.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7773509
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT521980.671485682977919No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT451920.5813590747756258No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT366640.47165314917626006No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274100.35260781199327096No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94700.12182400509216623No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90800.11680696581170744No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87440.11248459350854292No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85010.10935859211071859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC80000.015.40125137
CGTATAC2400.014.6458343
ATACCCG6250.013.0245
TAGATCG2602.3646862E-1112.8076935
TCTTATA122150.012.61604537
TATACAC33800.012.09615437
GTATTAC6700.011.3208951
CTCTTAT178200.011.25364837
GTACCGT4050.010.9629636
GTACTAG5400.010.9629631
GTATTAG7600.010.9539471
TAGACTG10200.010.8823535
ACCCGTT8450.010.7278117
TATACTG11350.010.5947135
TTATACA50800.010.56102437
TACCCGT7600.010.4671066
GGGTAAG22400.010.0758931
GGTATCA373800.010.0121721
TAGACAG12500.09.9165
TCTCTTA242500.09.90226737
Produced by FastQC (version 0.11.5)