##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727069.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7773509
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89727213282959	31.0	31.0	34.0	30.0	34.0
2	32.111845113963334	33.0	31.0	34.0	30.0	34.0
3	32.217313699643235	34.0	31.0	34.0	30.0	34.0
4	35.8504137578023	37.0	35.0	37.0	35.0	37.0
5	35.54926867647545	37.0	35.0	37.0	33.0	37.0
6	35.58120444705216	37.0	35.0	37.0	33.0	37.0
7	35.533986131617006	37.0	35.0	37.0	33.0	37.0
8	35.509086437026056	37.0	35.0	37.0	33.0	37.0
9	37.31460785598885	39.0	37.0	39.0	34.0	39.0
10	37.138117804970705	39.0	37.0	39.0	33.0	39.0
11	37.2298610575996	39.0	37.0	39.0	34.0	39.0
12	37.137231461364486	39.0	37.0	39.0	33.0	39.0
13	37.214583401138405	39.0	37.0	39.0	34.0	39.0
14	38.34892787800207	40.0	38.0	41.0	34.0	41.0
15	38.30550154376871	40.0	38.0	41.0	34.0	41.0
16	38.25071843359286	40.0	38.0	41.0	33.0	41.0
17	38.23374913439992	40.0	38.0	41.0	33.0	41.0
18	38.24746636300286	40.0	38.0	41.0	33.0	41.0
19	38.29716566868321	40.0	38.0	41.0	34.0	41.0
20	38.213259417336495	40.0	38.0	41.0	34.0	41.0
21	38.156349211147756	40.0	38.0	41.0	33.0	41.0
22	38.082445778347974	40.0	37.0	41.0	33.0	41.0
23	38.017884715898575	40.0	37.0	41.0	33.0	41.0
24	37.928031729300116	40.0	37.0	41.0	33.0	41.0
25	37.894464520463025	40.0	37.0	41.0	33.0	41.0
26	37.71712633252242	40.0	37.0	41.0	33.0	41.0
27	37.5617333176047	39.0	37.0	41.0	32.0	41.0
28	37.45805401395946	39.0	36.0	41.0	32.0	41.0
29	37.33531459216166	39.0	36.0	41.0	32.0	41.0
30	37.26375849053497	39.0	36.0	41.0	32.0	41.0
31	37.18278013185551	39.0	36.0	40.0	31.0	41.0
32	37.04891343150178	39.0	36.0	40.0	31.0	41.0
33	36.94978586890425	39.0	35.0	40.0	31.0	41.0
34	36.884401754728785	39.0	35.0	40.0	31.0	41.0
35	36.782100593181276	39.0	35.0	40.0	30.0	41.0
36	36.64158528664468	39.0	35.0	40.0	30.0	41.0
37	36.53465699981823	39.0	35.0	40.0	30.0	41.0
38	36.45953931487054	39.0	35.0	40.0	30.0	41.0
39	36.328795657147886	39.0	35.0	40.0	30.0	41.0
40	36.203948821568225	39.0	35.0	40.0	30.0	41.0
41	36.11505974972178	39.0	35.0	40.0	29.0	41.0
42	35.940388439763815	39.0	35.0	40.0	28.0	41.0
43	34.66135692388084	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	14.0
10	27.0
11	43.0
12	43.0
13	29.0
14	23.0
15	35.0
16	49.0
17	125.0
18	274.0
19	619.0
20	1326.0
21	2713.0
22	5019.0
23	9068.0
24	15123.0
25	24428.0
26	37463.0
27	55403.0
28	78690.0
29	109001.0
30	144727.0
31	188702.0
32	242587.0
33	315897.0
34	414770.0
35	557255.0
36	788365.0
37	1181666.0
38	1921829.0
39	1678194.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.4335253229912	18.43440330486528	13.147408718507947	24.984662653635574
2	18.274758542120427	21.438078993669397	35.792175708550666	24.49498675565951
3	20.630374262125383	23.152234081159488	29.799759670954263	26.41763198576087
4	15.214480358870105	15.69949941525764	33.438399569615214	35.64762065625704
5	15.821735074854871	35.47871366714826	33.197748918795874	15.501802339200996
6	35.10520152481975	33.63693281888527	14.87842877650235	16.37943687979264
7	28.649352563945058	30.254920911521427	21.65303983053213	19.44268669400138
8	26.3971264457274	34.73087893768438	19.721775584231008	19.150219032357203
9	26.30037477283425	15.272883841775961	20.07155327150197	38.35518811388782
10	17.898840793777946	26.608832639159484	32.372433092957124	23.119893474105453
11	34.49763806795618	22.272566996449093	21.246222265903338	21.983572669691384
12	21.41807515756398	25.632967042297118	30.138345501368814	22.810612298770092
13	31.06943080660227	19.720167558820606	24.797527088474457	24.412874546102667
14	22.07232280814237	21.21478215307913	26.68913099605339	30.023764042725105
15	25.277477648768397	27.33028288768946	23.56060821438555	23.83163124915659
16	23.644431362979063	25.97776628289747	25.889916638676304	24.48788571544717
17	23.430152328890337	26.562946026048213	26.49601357636558	23.510888068695877
18	23.548978974617512	25.0406348021209	27.500759309598795	23.9096269136628
19	24.473580721396218	25.344371505841185	27.800765394366945	22.38128237839565
20	23.873735786502596	25.336086958926785	27.547366318093925	23.242810936476694
21	23.282111077506954	26.023074006861	27.692706086787833	23.00210882884422
22	23.735831527306395	25.776531550937936	26.985162042007026	23.502474879748643
23	23.293495897412612	25.660818042405303	27.21682061473139	23.8288654454507
24	23.255816645996035	25.815883148781328	27.306162506533404	23.622137698689226
25	23.647068524652124	25.484436951188965	27.09126599068709	23.77722853347182
26	23.214098034748528	25.894136097353204	27.604702072127274	23.287063795770997
27	23.681441675824907	26.104311450594576	27.342246596742857	22.872000276837653
28	23.51396261328057	26.145824234589554	27.237622031440374	23.10259112068951
29	23.193348074852683	26.275752687750153	27.636721074099224	22.89417816329794
30	23.180689698821986	26.302214353903754	27.52929211248099	22.98780383479327
31	23.075177503492952	25.642718108385804	27.711076169076282	23.571028219044965
32	22.640483210349405	26.12652792966471	27.593111424969084	23.6398774350168
33	23.06321379443955	25.811908109966815	27.379411280028105	23.745466815565532
34	23.23031979508868	25.77411308072069	27.662693900528062	23.33287322366257
35	22.87808504499062	25.855453438080538	27.746993024643054	23.51946849228579
36	22.702951781492757	25.454141752456966	28.01162255038233	23.831283915667946
37	22.377899092932164	24.982372825451158	28.414465076196606	24.225263005420075
38	22.336630728799566	24.937283792943443	28.839395439048182	23.886690039208805
39	21.96901039157477	24.817402282547047	29.274848720185442	23.938738605692745
40	22.137145528486556	24.881723298963184	29.45467741788168	23.52645375466858
41	21.747218662768642	24.872332430566427	29.459089839607827	23.921359067057104
42	21.256848097815283	25.241547928998347	29.431894913867083	24.069709059319287
43	21.159105881269323	24.887177721155275	29.32119844461491	24.632517952960498
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7778.0
1	6510.5
2	5243.0
3	10574.0
4	15905.0
5	15905.0
6	19675.5
7	23446.0
8	21760.0
9	20074.0
10	28346.5
11	36619.0
12	36619.0
13	58489.0
14	80359.0
15	101060.0
16	121761.0
17	107677.0
18	93593.0
19	93593.0
20	98789.5
21	103986.0
22	83050.5
23	62115.0
24	70645.5
25	79176.0
26	79176.0
27	92316.5
28	105457.0
29	118733.5
30	132010.0
31	149254.0
32	166498.0
33	166498.0
34	189235.0
35	211972.0
36	240269.5
37	268567.0
38	302643.0
39	336719.0
40	336719.0
41	373626.0
42	410533.0
43	450244.5
44	489956.0
45	529963.0
46	569970.0
47	569970.0
48	609632.0
49	649294.0
50	664904.0
51	680514.0
52	683690.0
53	686866.0
54	686866.0
55	639909.0
56	592952.0
57	544627.5
58	496303.0
59	452479.0
60	408655.0
61	408655.0
62	358821.0
63	308987.0
64	262048.5
65	215110.0
66	179888.0
67	144666.0
68	144666.0
69	118901.5
70	93137.0
71	77232.5
72	61328.0
73	50029.0
74	38730.0
75	38730.0
76	31200.5
77	23671.0
78	19042.0
79	14413.0
80	11235.0
81	8057.0
82	8057.0
83	6457.5
84	4858.0
85	3696.0
86	2534.0
87	1835.5
88	1137.0
89	1137.0
90	758.0
91	379.0
92	248.0
93	117.0
94	81.0
95	45.0
96	45.0
97	29.5
98	14.0
99	9.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7773509.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.024736685058414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.57317932435122	41.91798438227365
2	9.99920371268099	10.40411880424974
3	3.4059546402954157	5.315816799678655
4	1.629327605617732	3.3906135862383677
5	0.9446067475741625	2.4571458656737626
6	0.6188642248167957	1.931774900393795
7	0.4198538679391025	1.5289950848014568
8	0.3289856335621985	1.3692312767392394
9	0.25956934030008816	1.2153623922563768
>10	1.6337174261690217	15.967538769763895
>50	0.12887623550266206	4.588963935816527
>100	0.0536397423640962	4.882811351998695
>500	0.0025429116347762365	0.9155282978274295
>1k	0.0014070510363322843	1.3366201068988222
>5k	1.72795735275266E-4	0.7039154854939417
>10k+	9.874042015729487E-5	2.07357895989553
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	52198	0.671485682977919	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	45192	0.5813590747756258	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	36664	0.47165314917626006	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27410	0.35260781199327096	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9470	0.12182400509216623	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9080	0.11680696581170744	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8744	0.11248459350854292	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8501	0.10935859211071859	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.859260984968307E-5
2	0.0	0.0	0.0	0.0	3.859260984968307E-5
3	0.0	0.0	0.0	0.0	3.859260984968307E-5
4	0.0	0.0	0.0	0.0	3.859260984968307E-5
5	0.0	0.0	0.0	0.0	3.859260984968307E-5
6	1.286420328322769E-5	0.0	0.0	0.0	6.432101641613845E-5
7	1.286420328322769E-5	0.0	0.0	0.0	6.432101641613845E-5
8	1.286420328322769E-5	0.0	0.0	1.286420328322769E-5	7.718521969936614E-5
9	3.859260984968307E-5	0.0	0.0	1.286420328322769E-5	1.0291362626582152E-4
10	6.432101641613845E-5	0.0	0.0	5.145681313291076E-5	1.0291362626582152E-4
11	6.432101641613845E-5	0.0	0.0	1.1577782954904922E-4	1.1577782954904922E-4
12	6.432101641613845E-5	0.0	0.0	1.415062361155046E-4	1.286420328322769E-4
13	7.718521969936614E-5	0.0	0.0	2.4441986238132613E-4	1.286420328322769E-4
14	1.0291362626582152E-4	0.0	0.0	3.6019769193037534E-4	1.8009884596518767E-4
15	1.0291362626582152E-4	0.0	0.0	4.7597552147942454E-4	2.4441986238132613E-4
16	1.1577782954904922E-4	0.0	0.0	9.13358433109166E-4	2.830124722310092E-4
17	1.1577782954904922E-4	0.0	0.0	0.0013893339545885907	2.830124722310092E-4
18	1.1577782954904922E-4	0.0	0.0	0.0018910378826344704	3.2160508208069227E-4
19	1.1577782954904922E-4	0.0	0.0	0.0028043963157436363	3.2160508208069227E-4
20	1.5437043939873227E-4	1.286420328322769E-5	0.0	0.004103680847349633	3.3446928536391994E-4
21	1.6723464268195997E-4	1.286420328322769E-5	0.0	0.007062447602492002	3.4733348864714766E-4
22	1.8009884596518767E-4	1.286420328322769E-5	0.0	0.010278498423298924	3.4733348864714766E-4
23	1.9296304924841537E-4	1.286420328322769E-5	0.0	0.013031437925909651	4.3738291162974147E-4
24	1.9296304924841537E-4	1.286420328322769E-5	0.0	0.018511588524564648	4.5024711491296914E-4
25	1.9296304924841537E-4	1.286420328322769E-5	0.0	0.023309936349208575	4.5024711491296914E-4
26	1.9296304924841537E-4	1.286420328322769E-5	0.0	0.0323791996638841	4.5024711491296914E-4
27	2.0582725253164304E-4	1.286420328322769E-5	0.0	0.06789726492887575	4.5024711491296914E-4
28	2.0582725253164304E-4	1.286420328322769E-5	0.0	0.16800649487895364	4.5024711491296914E-4
29	2.1869145581487074E-4	1.286420328322769E-5	0.0	0.32601750380683936	4.7597552147942454E-4
30	2.1869145581487074E-4	1.286420328322769E-5	0.0	0.5137962791321139	4.7597552147942454E-4
31	2.1869145581487074E-4	1.286420328322769E-5	0.0	0.9598110711649012	4.7597552147942454E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	8000	0.0	15.401251	37
CGTATAC	240	0.0	14.645834	3
ATACCCG	625	0.0	13.024	5
TAGATCG	260	2.3646862E-11	12.807693	5
TCTTATA	12215	0.0	12.616045	37
TATACAC	3380	0.0	12.096154	37
GTATTAC	670	0.0	11.320895	1
CTCTTAT	17820	0.0	11.253648	37
GTACCGT	405	0.0	10.962963	6
GTACTAG	540	0.0	10.962963	1
GTATTAG	760	0.0	10.953947	1
TAGACTG	1020	0.0	10.882353	5
ACCCGTT	845	0.0	10.727811	7
TATACTG	1135	0.0	10.594713	5
TTATACA	5080	0.0	10.561024	37
TACCCGT	760	0.0	10.467106	6
GGGTAAG	2240	0.0	10.075893	1
GGTATCA	37380	0.0	10.012172	1
TAGACAG	1250	0.0	9.916	5
TCTCTTA	24250	0.0	9.902267	37
>>END_MODULE