FastQCFastQC Report
Thu 9 Feb 2017
SRR2727068.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727068.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7447406
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT663380.8907531024896455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT544890.7316507250981079No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT467360.6275473634712543No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352920.47388312118340264No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129430.17379205591853056No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT118350.15891439247437297No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116150.1559603437760745No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115450.15502041919025228No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112760.15140842328187828No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT84660.11367716490815727No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG81270.10912524441396106No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG76070.10214294749071018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC45000.014.63555637
CTTATAC97300.013.82271437
TATACCG3350.013.2537325
ATACCCG10100.012.8217825
ACCCGTT11250.012.0044457
TCTTATA141200.011.59525537
TCGTTAC4000.011.123
ATACCGA3207.4578566E-1110.9843766
TAGTACT6600.010.9318184
TAGACAG13950.010.8745525
TACCCGT11750.010.7063836
CTCTTAT198700.010.62330137
GTATAGT8050.010.5714291
CTAGACA9400.010.4308514
TATACGA1604.8275915E-410.406255
TACACAG17700.010.3474595
CTATACT11000.010.259094
TTATACA65250.010.15019136
GTCTAGA8300.010.0301211
TACTTAC13200.09.95075831